Journal of Molecular Medicine

, Volume 81, Issue 6, pp 380–387 | Cite as

A novel gene, RSD-3/HSD-3.1, encodes a meiotic-related protein expressed in rat and human testis

  • Xiaodong Zhang
  • Huixian Liu
  • Yan Zhang
  • Yuan Qiao
  • Shiying Miao
  • Linfang Wang
  • Jianchao Zhang
  • Shudong Zong
  • S. S. Koide
Original Article

Abstract

The expression of stage-specific genes during spermatogenesis was determined by isolating two segments of rat seminiferous tubule at different stages of the germinal epithelium cycle delineated by transillumination-delineated microdissection, combined with differential display polymerase chain reaction to identify the differential transcripts formed. A total of 22 cDNAs were identified and accepted by GenBank as new expressed sequence tags. One of the expressed sequence tags was radiolabeled and used as a probe to screen a rat testis cDNA library. A novel full-length cDNA composed of 2228 bp, designated as RSD-3 (rat sperm DNA no.3, GenBank accession no. AF094609) was isolated and characterized. The reading frame encodes a polypeptide consisting of 526 amino acid residues, containing a number of DNA binding motifs and phosphorylation sites for PKC, CK-II, and p34cdc2. Northern blot of mRNA prepared from various tissues of adult rats showed that RSD-3 is expressed only in the testis. The initial expression of the RSD-3 gene was detected in the testis on the 30th postnatal day and attained adult level on the 60th postnatal day. Immunolocalization of RSD-3 in germ cells of rat testis showed that its expression is restricted to primary spermatocytes, undergoing meiosis division I. A human testis homologue of RSD-3 cDNA, designated as HSD-3.1 (GenBank accession no. AF144487) was isolated by screening the Human Testis Rapid-Screen arrayed cDNA library panels by RT-PCR. The exon-intron boundaries of HSD-3.1 gene were determined by aligning the cDNA sequence with the corresponding genome sequence. The cDNA consisted of 12 exons that span approximately 52.8 kb of the genome sequence and was mapped to chromosome 14q31.3.

Keywords

Spermatogenesis Spermatocyte-specific gene expression Meiosis 

Abbreviations

EST

Expressed sequence tag

IPTG

Isopropyl-β-D-thiogalactoside

PCR

Polymerase chain reaction

SC

Synaptonemal complex

Notes

Acknowledgements

These investigations were supported by grants from National Natural Science Foundation of China (30240019), National High Technology Research and Development Plan of China (2001AA221131), Special Fund for Major State Basic Research Project (G1999055901), State Ministry of Science and Technology Program (2002BA711A01).

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Xiaodong Zhang
    • 1
  • Huixian Liu
    • 1
  • Yan Zhang
    • 1
  • Yuan Qiao
    • 1
  • Shiying Miao
    • 1
  • Linfang Wang
    • 1
  • Jianchao Zhang
    • 2
  • Shudong Zong
    • 2
  • S. S. Koide
    • 3
  1. 1.National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical SciencesPeking Union Medical CollegeBeijingChina
  2. 2.National Research Institute for Family PlanningBeijingChina
  3. 3.Center for Biomedical ResearchPopulation CouncilNew YorkUSA

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