Journal of Molecular Medicine

, Volume 81, Issue 7, pp 428–434

Allele-specific differential expression of a common adiponectin gene polymorphism related to obesity

  • Wei-Shiung Yang
  • Pei-Ling Tsou
  • Wei-Jei Lee
  • Da-Lun Tseng
  • Chi-Ling Chen
  • Chi-Chung Peng
  • Kuan-Ching Lee
  • Mei-Ju Chen
  • Chang-Jen Huang
  • Tong-Yuan Tai
  • Lee-Ming Chuang
Original Article

Abstract

Adiponectin gene polymorphisms have recently been reported to be associated with obesity, insulin sensitivity, and the risk of type 2 diabetes. We examined a T94G polymorphism of the adiponectin gene in 245 ostensibly normal nondiabetic subjects. The G allele frequency was lower among subjects with higher BMI (≥27) than in those with lower BMI. BMI was inversely correlated with the dose of G allele. Multivariate linear regression analyses showed that the adiponectin genotypes were significantly related to BMI after adjusting for age and gender. The dose of the G allele was associated with a reduction of approximately 1.12 kg/m2 in BMI. We further found that the relative mRNA levels of G allele were consistently higher than those of T allele in the omental adipose tissue from 21 heterozygous subjects. Finally, we observed that the expression levels of adiponectin affected insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes. In conclusion, the allele-specific differential expression of this common polymorphism could be responsible for its biological effects observed in this and the other studies.

Keywords

Obesity Insulin resistance Adiponectin Polymorphism mRNA 

Abbreviations

BMI

Body mass index

SNP

Single nucleotide polymorphism

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Wei-Shiung Yang
    • 1
    • 3
  • Pei-Ling Tsou
    • 2
  • Wei-Jei Lee
    • 5
  • Da-Lun Tseng
    • 3
  • Chi-Ling Chen
    • 4
  • Chi-Chung Peng
    • 1
  • Kuan-Ching Lee
    • 1
  • Mei-Ju Chen
    • 1
  • Chang-Jen Huang
    • 6
  • Tong-Yuan Tai
    • 1
  • Lee-Ming Chuang
    • 1
    • 3
  1. 1.Division of Endocrinology and Metabolism, Departments of Internal MedicineNational Taiwan University HospitalTaipeiTaiwan
  2. 2.Department of Clinical PathologyNational Taiwan University HospitalTaipeiTaiwan
  3. 3.Graduate Institute of Clinical MedicineNational Taiwan University Medical CollegeTaipeiTaiwan
  4. 4.Institute of EpidemiologyNational Taiwan UniversityTaipeiTaiwan
  5. 5.Department of SurgeryEn-Chu-Kong HospitalTaipei HsienTaiwan
  6. 6.Institute of Biological ChemistryAcademia SinicaTaipeiTaiwan

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