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European Journal of Wood and Wood Products

, Volume 77, Issue 6, pp 1137–1150 | Cite as

Surface mould growth on wood: a comparison of laboratory screening tests and outdoor performance

  • Solrun Karlsen LieEmail author
  • Geir I. Vestøl
  • Olav Høibø
  • Lone Ross Gobakken
Original
  • 90 Downloads

Abstract

Laboratory screening tests are commonly used to indicate wood materials’ resistance or susceptibility to surface mould growth, but the results can deviate from what happens during outdoor exposure. In this study, the aim was to investigate how well agar plate screening tests and water uptake tests can predict mould growth on exterior wooden claddings. The tested wood materials included Norway spruce heartwood (Picea abies), sapwood and heartwood of Scots pine (Pinus sylvestris), aspen (Populus tremula), acetylated Radiata pine (Pinus radiata) and DMDHEU-modified Scots pine sapwood. The agar plate test included four inoculation methods (two monoculture spore suspensions of Aureobasidium species, one mixed-culture spore suspension, and inoculation from outdoor air) and three incubation temperatures (5, 16 and 27 °C). Inoculation method and incubation temperature had significant effects on the mould rating in the agar plate screening test, but none of the agar plate test combinations gave good indications of outdoor performance. Results from the agar plate test gave significantly negative correlations or no significant correlation with results from the outdoor test. However, the water uptake test gave significantly positive correlations with outdoor mould rating, and could be a useful indicator of susceptibility of uncoated wooden claddings to surface mould growth.

Notes

Acknowledgements

The authors gratefully acknowledge Holger Militz and Kim Christian Krause (Georg-August-Universität Göttingen, Germany) for providing the DMDHEU material, Thor-Erik Vatne Alstad (Norwegian Institute of Bioeconomy Research) for assistance in the preparation of the specimens, Sigrun Kolstad (Norwegian Institute of Bioeconomy Research) for assistance during the water uptake test and the laboratory test, Ingrid Grønli (Mycoteam AS) for analysing the air samples and Cathrine Marie Whist (Mycoteam AS) for helping with fungal identification.

Funding

This study was funded by the Research Council of Norway through the WoodBeBetter project (Project no. 225345).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

107_2019_1444_MOESM1_ESM.pdf (1 mb)
Supplementary material 1 (PDF 1030 kb)

References

  1. Adan OCG (1994) On the fungal defacement of interior finishes. PhD Dissertation, Eindhoven University of TechnologyGoogle Scholar
  2. Adan OCG, Huinink HP, Bekker M (2011) Water relations of fungi in indoor environments. In: Adan OCG, Samson RA (eds) Fundamentals of mold growth in indoor environments and strategies for healthy living, 1st edn. Wageningen Academic Publishers, Wageningen, pp 41–65Google Scholar
  3. Ahmed SA, Sehlstedt-Persson M, Morén T (2013) Development of a new rapid method for mould testing in a climate chamber: preliminary tests. Eur J Wood Prod 71:451–461Google Scholar
  4. Anusha B, Sathya K, Parthasarathy S, Prabakar K, Raghu D, Thiribhuvanamala G, Ramjegathesh R, Subramanian KS, Paliyath G, Jayasankar S (2016) Effect of hexanal on mycelial growth and spore germination of Colletotrichum gloeosporioides and Lasiodiplodia theobromae of mango. Trop Agric (Trinidad) 93:12–322Google Scholar
  5. ASTM 5590 (2010) Standard test method for determining the resistance of paint films and related coatings to fungal defacement by accelerated four-week agar plate assay. American Society for Testing and Materials (ASTM), PennsylvaniaGoogle Scholar
  6. ASTM D4445-10 (2015) Standard test method for fungicides for controlling sapstain and mould on unseasoned lumber (laboratory method). American Society for Testing and Materials (ASTM), PennsylvaniaGoogle Scholar
  7. Ayerst G (1969) The effects of moisture and temperature on growth and spore germination in some fungi. J Stored Prod Res 5:127–141Google Scholar
  8. Blom Å, Bergström M (2005) Mycologg: a new accelerated test method for wood durability above ground. Wood Sci Technol 39:663–673Google Scholar
  9. Blom Å, Bergström M (2006) Untreated Scots pine (Pinus sylvestris) and Norway spruce (Picea abies) wood-panels exposed out of ground contact in Sweden for two years. Holz Roh Werkst 64:53–61Google Scholar
  10. Blom Å, Thörnqvist T, Bergström M (2010) Outdoor exposure of untreated Scots pine (Pinus sylvestris L.) and Norway spruce [Picea abies (L.) Karst.] wood samples. Wood Mater Sci Eng 5:204–210Google Scholar
  11. Blom Å, Johansson J, Sivrikaya H (2013) Some factors influencing susceptibility to discoloring fungi and water uptake of Scots pine (Pinus sylvestris), Norway spruce (Picea abies) and Oriental spruce (Picea orientalis). Wood Mater Sci Eng 8:139–144Google Scholar
  12. Brischke C, Meyer L, Hesse C, Van Acker J, De Windt I, Van den Bulcke J, Conti E, Humar M, Viitanen H, Kutnik M, Malassenet L (2014) Moisture dynamics of wood and wood-based products – Results from an inter-laboratory test. IRG/WP 14-20539. The International Research Group on Wood Protection, StockholmGoogle Scholar
  13. BS 3900 Part G6 (1989) British Standard Methods of test for paints Part G6. Assessment of resistance to fungal growth. British Standards Institution (BSI), LondonGoogle Scholar
  14. Chedgy RJ, Morris PI, Lim YW, Breuil C (2007) Black stain of western red cedar (Thuja plicata Donn) by Aureobasidium pullulans: the role of weathering. Wood Fiber Sci 39:472–481Google Scholar
  15. Clausen CA, West M (2005) Test method for assessing resistance of pine lumber and waferboard to mold. Forest Prod J 55:164–166Google Scholar
  16. De Vetter L, Van den Bulcke J, De Windt I, Stevens M, Van Acker J (2009) Preventive action of organosilicon treatments against disfigurement of wood under laboratory and outdoor conditions. Int Biodeterior Biodegrad 63:1093–1101Google Scholar
  17. DIN 52617 (1987) Determination of the water absorption coefficient of construction materials. Beuth-Verlag, BerlinGoogle Scholar
  18. Eaton RA, Hale MDC (1993) Wood—decay, pests and protection. Chapmann and Hall, LondonGoogle Scholar
  19. Emmerich L, Bollmus S, Militz H (2019) Wood modification with DMDHEU (1.3-dimethylol-4.5-dihydroxyethyleneurea)—state of the art, recent research activities and future perspectives. Wood Mater Sci Eng 14:3–18Google Scholar
  20. EN 84, (1997) Wood preservatives—accelerated ageing of treated wood prior to biological testing. Leaching procedure. European Committee for Standardization (CEN), BrusselsGoogle Scholar
  21. EN 152 (2011) Wood preservatives—determination of the protective effectiveness of a preservative treatment against blue stain in wood in service—laboratory method. European Committee for Standardization (CEN), BrusselsGoogle Scholar
  22. EN 15457 (2014) Paints and varnishes—laboratory method for testing the efficacy of film preservatives in a coating against fungi. European Committee for Standardization (CEN), BrusselsGoogle Scholar
  23. Ghosh SC, Militz H, Mai C (2009) The efficacy of commercial silicones against blue stain and mould fungi in wood. Eur J Wood Prod 67:159–167Google Scholar
  24. Giordano L, Nicolotti G, Zanuttini R, Cremonini C, Gonthier P (2011) Effectiveness of two chemical preservatives against widespread moulds of high density fibreboard. Eur J Wood Prod 69:667–669Google Scholar
  25. Gjerdrum P, Høibø O (2014) Heartwood detection in Scots pine by means of heat-sensitive infrared images. Eur J Wood Prod 62:131–136Google Scholar
  26. Gobakken LR, Alfredsen G (2016) Susceptibility of wood substrates to Aurebasidium pullulans at different temperatures. IRG/WP 16-10863. The International Research Group on Wood Protection, StockholmGoogle Scholar
  27. Gobakken LR, Lebow PK (2010) Modelling mould growth on coated modified and unmodified wood substrates exposed outdoors. Wood Sci Technol 44:315–333Google Scholar
  28. Gobakken LR, Vestøl GI (2015) Mould growth on spruce claddings and the effect of selected influencing factors after 4 years of outdoor testing. IRG/WP 15-30671. The International Research Group on Wood Protection, StockholmGoogle Scholar
  29. Gobakken LR, Westin M (2008) Surface mould growth on five modified wood substrates coated with three different coating systems when exposed outdoors. Int Biodeterior Biodegrad 62:397–402Google Scholar
  30. Gobakken LR, Høibø OA, Solheim H (2010) Factors influencing surface mould growth on wooden claddings exposed outdoors. Wood Mater Sci Eng 5:1–12Google Scholar
  31. Gobakken LR, Bardage S, Long II CJ (2013) Surface moulds and staining fungi on acetylated wood - effect of increasing acetyl content. IRG/WP 13-10797. The International Research Group on Wood Protection, StockholmGoogle Scholar
  32. Gobakken LR, Gellerich A, Høibø O, Militz H (2014) Wood surface protection by wood modification systems and their resistance to mould and blue stain fungi. In: Nunes L, Jones D, Hill C, Militz H (eds) The seventh European conference on wood modification. Book of Abstracts, LisbonGoogle Scholar
  33. Godal JB (1994) Tre til tekking og kledning (Wood for roofing and cladding). Landbruksforlaget, OsloGoogle Scholar
  34. Gqaleni N, Smith JE, Lacey J, Gettinby G (1997) Effects of temperature, water activity, and incubation time on production of aflatoxins and cyclopiazonic acid by an isolate of Aspergillus flavus in surface agar culture. Appl Environ Microbiol 633:1048–1053Google Scholar
  35. Gu J-D (2016) Biodegradation testing: so many tests but very little new innovation. Appl Environ Biotechnol 1:92–95Google Scholar
  36. Hernandez V (2012) Role of non-decay fungi on the weathering of wood. PhD Dissertation, The University of British ColumbiaGoogle Scholar
  37. Hiscox J, Clarkson G, Savoury M, Powell G, Savva I, Lloyd M, Shipcott J, Choimes A, Cumbriu XA, Boddy L (2016) Effects of pre-colonisation and temperature on interspecific fungal interactions in wood. Fungal Ecol 21:32–42Google Scholar
  38. Hoog GS, Hermandies-Nijhof EJ (1977) The black yeasts and allied Hyphomycetes. Centraalbureau voor Schimmelcultures, BaarnGoogle Scholar
  39. Hoog GS, Guarro J, Gené J, Figueras MJ (2000) Atlas of clinical fungi. Centraalbureau voor Schimmelcultures, UtrechtGoogle Scholar
  40. Irbe I, Grinins J, Andersone I, Andersons B (2018) Susceptibility of thermo-hydro-treated birch plywood to mould and blue stain fungi. Wood Mater Sci Eng 13:296–304Google Scholar
  41. Johansson P, Ekstrand-Tobin A, Svensson T, Bok G (2012) Laboratory study to determine the critical moisture level for mould growth on building materials. Int Biodeterior Biodegrad 73:23–32Google Scholar
  42. Käärik A (1980) Fungi causing sap stain in wood. The Swedish University of Agricultural Sciences, Department of Forest Products, Uppsala, Report No. R 114Google Scholar
  43. Kroken (2018) Månedstabeller for siste 12 måneder (Monthly tables for the last 12 months); (Accessed periodically). https://www.nmbu.no/fakultet/realtek/laboratorier/bioklim/meteorologiske-data/siste-12-mdr
  44. Kučera B (1992) Skandinaviske normer for testing av små feilfrie prøver av heltre (Scandinavian norms for testing small clear samples of solid wood). Norwegian Forest Research Institute, Ås, SkogforskGoogle Scholar
  45. Kutnik M, Suttie E, Brischke C (2014) European standards on durability and performance of wood and wood-based products—trends and challenges. Wood Mater Sci Eng 9:122–133Google Scholar
  46. Lie SK, Vestøl GI, Høibø O, Gobakken LR (2019a) Surface mould growth on wooden claddings—effects of transient wetting, relative humidity, temperature and material properties. Wood Mater Sci Eng 14:129–141Google Scholar
  47. Lie SK, Vestøl GI, Høibø O, Gobakken LR (2019b) Visual appearance of unpainted wood: mould coverage, lightness and uniformity. Int Wood Prod J 10:9–15Google Scholar
  48. Mantanis G, Terzi E, Kartal SN, Papadopoulos AN (2014) Evaluation of mold, decay and termite resistance of pine wood treated with zinc-and copper-based nanocompounds. Int Biodeterior Biodegrad 90:140–144Google Scholar
  49. Meyer-Veltrup L, Brischke C, Alfredsen G, Humar M, Flæte PO, Isaksson T, Brelid PL, Westin M, Jermer J (2017) The combined effect of wetting ability and durability on outdoor performance of wood: development and verification of a new prediction approach. Wood Sci Technol 51:615–637Google Scholar
  50. Morrell JJ, Sexton CM (1993) Fungal staining of ponderosa pine sapwood: effects of wood preconditioning and bioprotectants. Wood Fiber Sci 25:322–325Google Scholar
  51. Nielsen KF, Holm G, Uttrup LP, Nielsen PA (2004) Mould growth on building materials under low water activities. Influence of humidity and temperature on fungal growth and secondary metabolism. Int Biodeterior Biodegrad 54:325–336Google Scholar
  52. Pasanen A-L, Kasanen J-P, Rautiala S, Ikäheimo M, Rantamäki J, Kääriäinen H, Kalliokoski P (2000) Fungal growth and survival in building materials under fluctuating moisture and temperature conditions. Int Biodeterior Biodegrad 46:117–127Google Scholar
  53. Pfeffer A, Dieste A, Mai C, Militz H (2011) Effects of water glass and DMDHEU treatment on the colonisation of wood by Aureobasidium pullulans. Eur J Wood Prod 69:303–309Google Scholar
  54. Reinprecht L, Vacek V, Grznàrik T (2017) Enhanced fungal resistance of Scots pine (Pinus sylvestris L.) sapwood by treatment with methyltrimethoxysilane and benzalkoniumchloride. Eur J Wood Prod 75:817–824Google Scholar
  55. Roffael E (2006) Volatile organic compounds and formaldehyde in nature, wood and wood based panels. Holz Roh Werkst 64:144–149Google Scholar
  56. Roffael E, Schneider T, Dix B (2015) Effect of oxidising and reducing agents on the release of volatile organic compounds (VOCs) from strands made of Scots pine (Pinus sylvestris L.). Wood Sci Technol 49:957–967Google Scholar
  57. Sandberg K (2008) Degradation of Norway spruce (Picea abies) heartwood and sapwood during 5.5 years’ above-ground exposure. Wood Mater Sci Eng 3:83–93Google Scholar
  58. Sandberg D, Kutnar A, Mantanis G (2017) Wood modification technologies—a review. iForest 10:895–908Google Scholar
  59. Schmitt JA (1974) The microecology of mold growth. J Paint Technol 46:59–64Google Scholar
  60. Schoeman M, Dickinson D (1997) Growth of Aureobasidium pullulans on lignin breakdown products at weathered wood surfaces. Mycologist 11:168–172Google Scholar
  61. Sedlbauer K (2001) Prediction of mould fungus formation of the surface of and inside building components. PhD Dissertation, University of StuttgartGoogle Scholar
  62. Song J, Hildebrand PD, Fan L, Forney CF, Renderos WE, Campbell-Palmer L, Doucette C (2007) Effect of hexanal vapor on the growth of postharvest pathogens and fruit decay. J Food Sci 72:108–112Google Scholar
  63. Stefanowski BK, Curling SF, Ormondroyd GA (2017) A rapid screening method to determine the susceptibility of bio-based construction and insulation products to mould growth. Int Biodeterior Biodegrad 116:124–132Google Scholar
  64. Sun F, Bao B, Ma L, Chen A, Duan X (2012) Mould-resistance of bamboo treated with the compound of chitosan-copper complex and organic fungicides. J Wood Sci 58:51–56Google Scholar
  65. Thiis TK, Burud I, Flø A, Kraniotis D, Charisi S, Stefansson P (2017) Monitoring and simulation of diurnal surface conditions of a wooden facade. Procedia Environ Sci 38:331–339Google Scholar
  66. Tiitta M, Tomppo L, Järnström H, Löija M, Laakso T, Harju A, Venäläinen M, Iitti H, Paajanen L, Saranpää P, Lappalainen R, Viitanen H (2009) Spectral and chemical analyses of mould development on Scots pine heartwood. Eur J Wood Prod 67:151–158Google Scholar
  67. Tveite B (1977) Site-index curves for Norway spruce (Picea abies (L.) Karst)). Meddelelser Norsk institutt for Skogforskning 33:1–84Google Scholar
  68. Vainio-Kaila T, Rautkari L, Nordström K, Närhi M, Natri O, Kairi M (2013) Effect of extractives and thermal modification on antibacterial properties of Scots pine and Norway spruce. Int Wood Prod J 4:248–252Google Scholar
  69. Vainio-Kaila T, Hänninen T, Kyyhkynen A, Ohlmeyer M, Siitonen A, Rautkari L (2017) Effect of volatile organic compounds from Pinus sylvestris and Picea abies on Staphylococcus aureus, Escherichia coli, Streptococcus pneumoniae and Salmonella enterica serovar Typhimurium. Holzforschung 71:905–912Google Scholar
  70. van Laarhoven KA (2016) Water relations of Penicillum rubens on porous substrates. PhD Dissertation, Eindhoven University of TechnologyGoogle Scholar
  71. van Laarhoven KA, Huinink HP, Segers FJJ, Dijksterhuis J, Adan OCG (2015) Separate effects of moisture content and water activity on the hyphal extension of Penicillium rubens on porous media. Environ Microbiol 17:5089–5099PubMedGoogle Scholar
  72. Verdier T, Coutand M, Bertron A, Roques C (2014) A review of indoor microbial growth across building materials and sampling and analysis methods. Build Environ 80:136–149Google Scholar
  73. Vestøl GI, Sivertsen MS (2011) Effects of outdoor weathering and wood properties on liquid water absorption in uncoated Norway spruce cladding. For Prod J 61:352–358Google Scholar
  74. Viitanen H (1996) Factors affecting the development of mould and brown rot decay in wooden materials and wooden structures. Effect of humidity, temperature and exposure time. PhD Dissertation, The Swedish University of Agricultural SciencesGoogle Scholar
  75. Viitanen H, Ritschkoff A-C (1991) Mould growth in pine and spruce sapwood in relation to air humidity and temperature, Report No. 221. The Swedish University of Agricultural Sciences, Department of Forest Products, Uppsala, p 221Google Scholar
  76. Viitanen H, Vinha J, Salminen K, Ojanen T, Peuhkuri R, Paajanen L, Lähdesmäki K (2010) Moisture and bio-deterioration risk of building materials and structures. J Build Phys 33:201–224Google Scholar
  77. Wolff M, Thue-Hansen V, Grimenes AA (2016) Meterologiske data for Ås 2016 (Meteorological data for Ås 2016). Norges miljø-og Biovitenskapelige Universitet, ÅsGoogle Scholar
  78. Zabel R, Morrell J (1992) Wood microbiology. Decay and its prevention. Academic Press, San DiegoGoogle Scholar
  79. Žlahtič M, Humar M (2016) Influence of artificial and natural weathering on the hydrophobicity and surface properties of wood. BioResources 11:4964–4989Google Scholar
  80. Žlahtič M, Humar M (2017) Influence of artificial and natural weathering on the moisture dynamic of wood. BioResources 12:117–142Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Solrun Karlsen Lie
    • 1
    Email author
  • Geir I. Vestøl
    • 1
  • Olav Høibø
    • 1
  • Lone Ross Gobakken
    • 2
  1. 1.Faculty of Environmental Sciences and Natural Resource ManagementNorwegian University of Life SciencesÅsNorway
  2. 2.Norwegian Institute of Bioeconomy ResearchÅsNorway

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