Advertisement

European Journal of Wood and Wood Products

, Volume 72, Issue 5, pp 651–657 | Cite as

Antitermitic activity of extracts from Chamaecyparis obtusa branch heartwood

  • Takuya Morikawa
  • Tatsuya Ashitani
  • Hisayoshi Kofujita
  • Koetsu Takahashi
Original

Abstract

The antitermitic activity of extracts from the branch heartwood of Chamaecyparis obtusa (hinoki) against Japanese termites (Reticulitermes speratus) was compared with that of the trunk. Samples of branch and trunk heartwood were extracted with n-hexane, ethyl acetate, and methanol successively. n-Hexane extracts of branch and trunk heartwood were strongly antitermitic, and branch heartwood contained greater quantities of active n-hexane extracts than trunk heartwood. Germacra-1-(10), 5-dien-4β-ol, t-cadinol, α-cadinol, hinokiresinol, and hinokinin were separated from the branch extracts and the termiticidal and antifeedant activity of these compounds was tested by no-choice and dual-choice test methods. The sesquiterpenoids, germacra-1-(10), 5-dien-4β-ol, t-cadinol and α-cadinol were strongly termiticidal. The norlignan hinokiresinol and lignan hinokinin had weak termiticidal, and strong antifeedant and repellent activity. High concentrations of germacra-1-(10), 5-dien-4β-ol and hinokiresinol were present in branch heartwood. These compounds protect hinoki branches from termites and other harmful organisms. Hinoki branch heartwood, which is usually unused, is a potential source of active antitermitic compounds.

Keywords

Sesquiterpene Antifeedant Activity Harmful Organism Sesquiterpene Compound Termiticidal Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The study was supported by LIXIL JS Foundation (Grant-in-Aid for Research 12–85).

References

  1. Ali N, Ahmed G, Shah SWA, Ismail Shah, Ghias M, Imran Khan (2011) Acute toxicity, brine shrimp cytotoxicity and relaxant activity of fruits of Callistemon citrinus curtis. BMC Compl Alternative Med 11:99CrossRefGoogle Scholar
  2. Ashitani T, Kusumoto N, Borg-Karlson Anna-Karin, Koki Fujita, Takahashi K (2013) Antitermite activity of β-caryophyllene epoxide and episulfide. Z Naturforsch C 68(7–8):302–306PubMedCrossRefGoogle Scholar
  3. El-Shazly AM, Hussein KT (2004) Chemical analysis and biological activity of the essential oil of Teucrium leucocladum Boiss (Lamiaceae). Biochem Syst Ecol 32(7):665–674CrossRefGoogle Scholar
  4. Hong EJ, Na KJ, Choi IG, Choi KC, Jeung EB (2004) Antibacterial and antifungal effects of essential oils from coniferous trees. Biol Pharm Bull 27(6):863–866PubMedGoogle Scholar
  5. Ishii T, Kadoya K ((1993)) Phytotoxic constituents in the bark and sawdust extracts of Chamaecyparis obtusa and Cryptomeria japonica and their effects on the growth of seedlings of trifoliate orange (Poncirus trifoliata Raf.) and rice (Oryza sativa L.). Engei Gakkai Zasshi 62(2):285–294CrossRefGoogle Scholar
  6. Japan Forestry agency (2011) Annual report on trends in forests and forestry fiscal year 2011. Tokyo, Japan, pp 55–56Google Scholar
  7. Kijidani Y, Sakai N, Kimura K, Fujisawa Y, Hiraoka Y, Matsumura J, Koga S (2012) Termite resistance and color of heartwood of hinoki (Chamaecyparis obtusa) trees in five half-sib families in a progeny test stand in Kyushu. Japan. J Wood Sci 58(6):471–478CrossRefGoogle Scholar
  8. Kinjo K, Yaga S (1986) Study on the cultivation culture media of basidiomycetes IV. Antifungal activity of hinoki. Mokuzai Gakkaishi 32(8):632–634Google Scholar
  9. Kinjo K, Doufuku Y, Yaga S (1988) Termiticidal substances from the wood of Chamaecyparis obtusa Endl. Mokuzai Gakkaishi 34(5):451–455Google Scholar
  10. Kondo R, Imamura H (1986) Antifungal compounds in heartwood extractives of hinoki (Chamaecyparis obtusa Endl). Mokuzai Gakkaishi 32(3):213–217Google Scholar
  11. Kusumoto N, Ashitani T, Hayasaka Y, Murayama T, Ogiyama K, Takahashi K (2009) Antitermitic activity of abietane-type diterpenes from Taxodium distichum Cones. J Chem Ecol 35(12):635–642PubMedCrossRefGoogle Scholar
  12. Matsushima N, Kang HY, Sameshima K, Takamura N (1990) The complexity of termiticidal activity in hinoki (Chamaecyparis obtusa Endl) wood. Mokuzai Gakkaishi 36(7):559–564Google Scholar
  13. McDaniel CA (1989) Major termiticidal components of heartwood of Port-Orford-cedar, Chamaecyparis lawsoniana (A. Murr.) Parl. Mat Organismen 24:1–15Google Scholar
  14. Medarde M, Gordaliza M, Lucas MJ (1995) Structure elucidation of germacrane alcohols from Juniperus communis subsp. Hemisphaerica. J Nat Prod 58(7):1059–1064CrossRefGoogle Scholar
  15. Morikawa T, Ashitani T, Sekine N, Kusumoto N, Takahashi K (2012) Bioactivity of extracts from Chamaecyparis obtusa branch heartwood. J Wood Sci 58(6):544–549CrossRefGoogle Scholar
  16. Morita Y, Matsumura E, Okabe T, Fukui T, Shibata M, Sugiura M, Ohe T, Tsujibo H, Ishida N, Inamori Y (2004) Biological activity of α-thujaplicin, the isomer of hinokitiol. Biol Pharm Bull 27(6):899–902PubMedGoogle Scholar
  17. Nakashima Y, Shimizu K (1972) Studies on an antitermitic activity of Hinokiasunaro (Thujopsis dolabrata Sieb. et Zucc. var. Hondai Makino). III. The components with a termiticidal activity. Miyazaki Daigaku Nogakubu Kenkyu Hokoku 19:251–259Google Scholar
  18. Ohira T, Yatagai M (1994) Allelopathic compounds produced by forest plants II. The relationships between the inhibition effects on plant growth and killing activity of brine shrimp on phenolic compounds. Mokuzai Gakkaishi 40(5):541–548Google Scholar
  19. Ohmura W, Doi S, Aoyama M, Ohara S (2000) Antifeedant activity of flavonoids and related compounds against the subterranean termite Coptotermes formosanus Shiraki. J Wood Sci 46(2):149–153CrossRefGoogle Scholar
  20. Ohtani Y, Hazama M, Sameshima K (1996) Crucial Chemical Factors for Termiticidal Activity of Hinoki Wood (Chamaecyparis obtusa) II. Variations in termiticidal activity among five individual samples of hinoki wood. Mokuzai Gakkaishi 42(12):1228–1233Google Scholar
  21. Sakasegawa M, Hori K, Yatagai M (2003) Composition and antitermite activity of essential oils from Melaleuca species. J Wood Sci 49(2):181–187CrossRefGoogle Scholar
  22. Seru G, Pannakal ST, Serge F, Hartmut L (2004) Antitermitic quinones from Diospyros sylvatica. Phytochemistry 65(9):1265–1271CrossRefGoogle Scholar
  23. Shiei B, Iizuka Y, Matsubara Y (1981) Monoterpenoid and sesquiterpenoid constituents of the essential oil of hinoki (Chamaecyparis obtusa (Sieb. et Zucc.) Endl.). Agric Biol Chem 45(6):1497–1499CrossRefGoogle Scholar
  24. Tanaka K, Manabe M, Matsuura S (1979) Biological test using brine shrimp (part2). Rept Natl Food Res Inst 34:84–88Google Scholar
  25. Yanga JK, Choia MS, Seob WT, Rinker DL, Hand SW, Cheonge GW (2007) Chemical composition and antimicrobial activity of Chamaecyparis obtusa leaf essential oil. Fitoterapia 78(2):149–152CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Takuya Morikawa
    • 1
    • 3
  • Tatsuya Ashitani
    • 1
    • 2
  • Hisayoshi Kofujita
    • 1
  • Koetsu Takahashi
    • 1
    • 2
  1. 1.The United Graduate School of Agricultural ScienceIwate UniversityMoriokaJapan
  2. 2.Faculty of AgricultureYamagata UniversityTsuruokaJapan
  3. 3.Sanriku Fisheries Research Center, United Graduate SchoolIwate UniversityMoriokaJapan

Personalised recommendations