Mesenchymal stem cells in peripheral blood of severely injured patients
- 261 Downloads
Mesenchymal stem cells (MSCs) are primarily stromal cells present in bone marrow and other tissues that are crucial for tissue regeneration and can be mobilized into peripheral blood after different types of organ damage. However, little is known about MSC appearance in blood in the setting of polytrauma.
We conducted a monocentered and longitudinal observational clinical study in 11 polytraumatized patients with an injury severity score (ISS) ≥ 24 to determine the numbers of MSCs in peripheral blood. Blood was collected from healthy volunteers and patients after polytrauma in the emergency room and 4, 12, 24, 48 h, 5 and 10 day later, and cells carrying MSC-surface markers (negative for CD45, positive for CD29, CD73, CD90, CD105, and CD166 in different combinations also employing the more stringent markers STRO1 and MSCA1) were detected and characterized using flow cytometry. Relative numbers of MSC-like cells were correlated with clinical parameters to evaluate if specific injury patterns had an influence on their presence in the blood cell pool.
We were able to detect MSC marker-positive cells in both cohorts; however, the percentage of those cells present in the blood of patients during the first 10 day after injury was mostly similar to healthy volunteers, and significantly lowers starting at 4 h post trauma for one marker combination when compared to controls. Furthermore, the presence of a pelvis fracture was partly correlated with reduced relative numbers of MSC-like cells detectable in blood.
Polytrauma in humans was associated with partly reduced relative numbers of MSC-like cells detected in peripheral blood in the time course after injury. Further studies need to define if this reduction was due to lower mobilization from the bone marrow or to active migration to the sites of injury.
KeywordsMesenchymal stem cells MSC-like cells Trauma Polytrauma Fracture
This work was supported in part by a research grant from the German Ministry of Defense, Berlin, Germany (Vertragsforschungsvorhaben AZ E/U2AD/CD525/DF559).
Compliance with ethical standards
Conflict of interest
Rebecca Wiegner, Nina-Emily Hengartner, Eberhard Barth, Florian Gebhard, Lorenz Lampl, Markus S. Huber-Lang, and Rolf E. Brenner declare that they have no conflict of interest.
Statement of human and animal rights
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
- 12.Dominici M, Le BK, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. Int Soc Cell Ther Pos Statement Cytother. 2006;8:315–7.Google Scholar
- 26.Mansilla E, Marin GH, Drago H, Sturla F, Salas E, Gardiner C, et al. Bloodstream cells phenotypically identical to human mesenchymal bone marrow stem cells circulate in large amounts under the influence of acute large skin damage: new evidence for their use in regenerative medicine. Transpl Proc. 2006;38:967–9.CrossRefGoogle Scholar
- 29.Xu L, Li G. Circulating mesenchymal stem cells and their clinical implications. J Orthop Transl. 2014;2:1–7.Google Scholar
- 32.Wang Y, Johnsen HE, Mortensen S, Bindslev L, Ripa RS, Haack-Sorensen M, et al. Changes in circulating mesenchymal stem cells, stem cell homing factor, and vascular growth factors in patients with acute ST elevation myocardial infarction treated with primary percutaneous coronary intervention. Heart. 2006;92:768–74.CrossRefPubMedGoogle Scholar
- 38.Marlicz W, Zuba-Surma E, Kucia M, Blogowski W, Starzynska T, Ratajczak MZ. Various types of stem cells, including a population of very small embryonic-like stem cells, are mobilized into peripheral blood in patients with Crohn’s disease. Inflamm Bowel Dis. 2012;18:1711–22.CrossRefPubMedGoogle Scholar
- 44.Sielatycka K, Poniewierska-Baran A, Nurek K, Torbe A, Ratajczak MZ. Novel view on umbilical cord blood and maternal peripheral blood-an evidence for an increase in the number of circulating stem cells on both sides of the fetal-maternal circulation barrier. Stem Cell Rev. 2017. doi: 10.1007/s12015-017-9763-z.PubMedPubMedCentralCrossRefGoogle Scholar