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Strahlentherapie und Onkologie

, Volume 191, Issue 6, pp 511–517 | Cite as

Effect of the coffee ingredient cafestol on head and neck squamous cell carcinoma cell lines

  • Ulana Kotowski
  • Gregor HeiduschkaEmail author
  • Rudolf Seemann
  • Julia Eckl-Dorna
  • Rainer Schmid
  • Veronika Kranebitter
  • Isabella Stanisz
  • Markus Brunner
  • Claudia Lill
  • Dietmar Thurnher
Original Article

Abstract

Background and purpose

Cafestol is a diterpene molecule found in coffee beans and has anticarcinogenic properties. The aim of the study was to examine the effects of cafestol in head and neck squamous cell carcinoma (HNSCC) cells.

Materials and methods

Three HNSCC cell lines (SCC25, CAL27 and FaDu) were treated with increasing doses of cafestol. Then combination experiments with cisplatin and irradiation were carried out. Drug interactions and possible synergy were calculated using the combination index analysis. Clonogenic assays were performed after irradiation with 2, 4, 6 and 8 Gy, respectively, and the rate of apoptosis was measured with flow cytometry.

Results

Treatment of HNSCC cells with cafestol leads to a dose-dependent reduction of cell viability and to induction of apoptosis. Combination with irradiation shows a reduction of clonogenic survival compared to each treatment method alone. In two of the cell lines a significant additive effect was observed.

Conclusion

Cafestol is a naturally occurring effective compound with growth-inhibiting properties in head and neck cancer cells. Moreover, it leads to a significant inhibition of colony formation.

Keywords

Phytochemicals Irradiation Head and neck cancer Cytotoxins Cisplatin 

Wirkung des Kaffeebestandteils Cafestol auf Kopf-Hals-Tumorzelllinien

Zusammenfassung

Hintergrund und Ziel

Cafestol ist ein Diterpen, das in der Kaffeebohne vorkommt und antikanzerogene Eigenschaften besitzt. Ziel der Studie war, die Wirkung von Cafestol auf Kopf-Hals-Tumorzelllinien zu untersuchen.

Material und Methodik

Drei Kopf-Hals-Tumorzelllinien (SCC25, CAL27 und FaDu) wurden mit steigenden Cafestol-Dosen behandelt. Anschließend fanden Kombinationsexperimente mit Cisplatin und Bestrahlung statt. Die Wechselwirkung zwischen den Substanzen und mögliche synergistische Wirkungen wurden mit dem Combination-Index analysiert. Koloniebildungstests wurden nach Bestrahlung mit 2, 4, 6 und 8 Gy durchgeführt. Apoptose wurde mittels Durchflusszytometrie gemessen.

Ergebnisse

Die Behandlung der Kopf-Hals-Tumorzelllinien mit Cafestol führt zu einer dosisabhängigen Abnahme des Zellüberlebens und zur Induktion von Apoptose. Die Kombination von Cafestol mit Bestrahlung zeigt eine geringere Koloniebildung verglichen mit den einzelnen Behandlungsmethoden. In 2 Zelllinien konnten wir einen signifikanten additiven Effekt nachweisen.

Schlussfolgerung

Cafestol ist ein natürlich vorkommender, effektiver und vielversprechender Wirkstoff mit wachstumshemmenden Eigenschaften in Kopf-Hals-Tumorzelllinien. Zudem führt er zu einer signifikanten Inhibition der Koloniebildung.

Schlüsselwörter

Phytochemikalien Bestrahlung Kopf- und Halstumoren Zytotoxine Cisplatin 

Notes

Compliance with ethical guidelines

Conflict of interest

U. Kotowski, G. Heiduschka, R. Seemann, J. Eckl-Dorna, R. Schmid, V. Kranebitter, I. Stanisz, M. Brunner, C. Lilland D. Thurnher state that there are no conflicts of interest.

The accompanying manuscript does not include studies on humans or animals.

References

  1. 1.
    Breitmaier E (2006) Terpenes. Wiley - VCH Verlag GmbH & Co. KGaA, Weinheim, GermanyGoogle Scholar
  2. 2.
    Cavin C, Holzhaeuser D, Scharf G, Constable A, Huber WW, Schilter B (2002) Cafestol and kahweol, two coffee specific diterpenes with anticarcinogenic activity. Food Chem Toxicol 40:1155–1163CrossRefPubMedGoogle Scholar
  3. 3.
    Choi MJ, Park EJ, Oh JH et al (2011) Cafestol, a coffee-specific diterpene, induces apoptosis in renal carcinoma Caki cells through down-regulation of anti-apoptotic proteins and Akt phosphorylation. Chem Biol Interact 190:102–108CrossRefPubMedGoogle Scholar
  4. 4.
    Chou T-C (2006) Theoretical basis, experimental design, and computerized simulation of synergism and antagonism in drug combination studies. Pharmacol Rev 58:621–681CrossRefPubMedGoogle Scholar
  5. 5.
    Corry J, Peters LJ, Rischin D (2010) Optimising the therapeutic ratio in head and neck cancer. Lancet Oncol 11:287–291CrossRefPubMedGoogle Scholar
  6. 6.
    Eder-Czembirek C, Erovic BM, Czembirek C et al (2010) Betulinic acid a radiosensitizer in head and neck squamous cell carcinoma cell lines. Strahlenther Onkol 186:143–148CrossRefPubMedGoogle Scholar
  7. 7.
    Ferlay J, Shin H-R, Bray F, Forman D, Mathers C, Parkin DM (2010) Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127:2893–2917CrossRefPubMedGoogle Scholar
  8. 8.
    Franken NAP, Rodermond HM, Stap J, Haveman J, van Bree C (2006) Clonogenic assay of cells in vitro. Nat Protoc 1:2315–2319CrossRefPubMedGoogle Scholar
  9. 9.
    González-Vallinas M, González-Castejón M, Rodríguez-Casado A, Ramírez de Molina A (2013) Dietary phytochemicals in cancer prevention and therapy: a complementary approach with promising perspectives. Nutr Rev 71:585–599CrossRefPubMedGoogle Scholar
  10. 10.
    Haddad RI, Shin DM (2008) Recent advances in head and neck cancer. N Engl J Med 359:1143–1154CrossRefPubMedGoogle Scholar
  11. 11.
    Harvey A (2008) Natural products in drug discovery. Drug Discovery Today 13:894–901CrossRefPubMedGoogle Scholar
  12. 12.
    Heiduschka G, Lill C, Seemann R et al (2014) The effect of resveratrol in combination with irradiation and chemotherapy: study using Merkel cell carcinoma cell lines. Strahlenther Onkol 190:75–80CrossRefPubMedGoogle Scholar
  13. 13.
    Joseph B, Vishwanath L, Venugopal BK (2014) Radiosensitization in head and neck cancer: do we have an alternative to platins? Role of taxanes. Oral Surg Oral Med Oral Pathol Oral Radiol 117:324–328CrossRefPubMedGoogle Scholar
  14. 14.
    Kotowski U, Heiduschka G, Brunner M et al (2011) Radiosensitization of head and neck cancer cells by the phytochemical agent sulforaphane. Strahlenther Onkol 187:575–580CrossRefPubMedGoogle Scholar
  15. 15.
    Lee K-A, Chae J-I, Shim J-H (2012) Natural diterpenes from coffee, cafestol and kahweol induce apoptosis through regulation of specificity protein 1 expression in human malignant pleural mesothelioma. J Biomed Sci 19:60CrossRefPubMedCentralPubMedGoogle Scholar
  16. 16.
    Mishra BB, Tiwari VK (2011) Natural products: an evolving role in future drug discovery. Eur J Med Chem 46:4769–4807CrossRefPubMedGoogle Scholar
  17. 17.
    Molinari G (2009) Natural products in drug discovery: present status and perspectives. Adv Exp Med Biol 655:13–27PubMedGoogle Scholar
  18. 18.
    Nambiar D, Rajamani P, Singh RP (2011) Effects of phytochemicals on ionization radiation-mediated carcinogenesis and cancer therapy. Mutat Res 728:139–157CrossRefPubMedGoogle Scholar
  19. 19.
    Rahman MA, Amin ARMR, Shin DM (2010) Chemopreventive potential of natural compounds in head and neck cancer. Nutr Cancer 62:973–987CrossRefPubMedCentralPubMedGoogle Scholar
  20. 20.
    Tribius S, Sommer J, Prosch C et al (2013) Xerostomie nach Strahlentherapie. Strahlenther Onkol 189:216–222CrossRefPubMedGoogle Scholar
  21. 21.
    Trotti A (2000) Toxicity in head and neck cancer: a review of trends and issues. Radiat Oncol Biol 47:1–12CrossRefGoogle Scholar
  22. 22.
    Urgert R, Katan MB (1996) The cholesterol-raising factor from coffee beans. J R Soc Med 89:618–623PubMedCentralPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Ulana Kotowski
    • 1
  • Gregor Heiduschka
    • 1
    Email author
  • Rudolf Seemann
    • 2
  • Julia Eckl-Dorna
    • 1
  • Rainer Schmid
    • 3
  • Veronika Kranebitter
    • 1
  • Isabella Stanisz
    • 1
  • Markus Brunner
    • 1
  • Claudia Lill
    • 1
  • Dietmar Thurnher
    • 1
  1. 1.Department of Otorhinolaryngology, Head and Neck SurgeryMedical University of ViennaViennaAustria
  2. 2.Departement of Cranio-, Maxillofacial- and Oral SurgeryMedical University of ViennaViennaAustria
  3. 3.Department of RadiotherapyMedical University of ViennaViennaAustria

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