Acetone effects on Buddleja scordioides polyphenol extraction process and assessment of their cellular antioxidant capacity and anti-inflammatory activity
The use of water, acetone, and hydroacetonic solutions as an extraction solvent for polyphenol compounds from Buddleja scordioides has been investigated. The phenolic profiles determined by liquid chromatography—electrospray ionization—mass spectrometry/mass spectrometry (LC–ESI–MS/MS) were compared with those obtained with the traditional aqueous infusion used for the treatment of gastrointestinal disorders. It was evaluated the effects of polyphenol extract at physiologically relevant conditions against H2O2-induced oxidative stress via attenuation of reactive oxygen species (ROS) levels and lipopolysaccharide (LPS)-induced inflammation in human HT-29 cells. The use of hydroacetonic solutions allows obtaining phytochemical extracts enriched with constituents that have antioxidant and anti-inflammatory activity in intestinal cells. This suggests that polyphenols of low and middle polarity from Buddleja scordioides may be better extracted with hydroacetonic solutions, and showing high nutraceutical potential to reduce oxidative stress associated with the onset and progress of inflammatory diseases.
KeywordsBuddleja scordioides Polyphenols Antioxidant Anti-inflammatory activity
Funding from the Strengthening and Development of Scientific and Technological Infrastructure program (Grants numbers 253333, 280172, and 224651) from the Mexican Science and Technology Council (CONACyT) is recognized. Financial support from TecNM/Instituto Tecnológico de Durango (Grant No. 5557.15) is also acknowledged. Authors CV-N and KMH-R are thankful for graduate scholarships granted by CONACyT for PhD studies.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Dent M, Dragovic-Uzelac V, Penic M, Brncic M, Bosiljkov T, Levaj B (2013) The effect of extraction solvents, temperature and time on the composition and mass fraction of polyphenols in Dalmatian wild sage (Salvia officinalis L.) extracts. Food Technol Biotechnol 51:84–91Google Scholar
- Díaz-Rivas JO, González-Laredo RF, Chávez-Simental JA, Montoya-Ayón JB, Moreno-Jiménez MR, Gallegos-Infante JA, Rocha-Guzmán NE (2018) Comprehensive characterization of extractable phenolic compounds by UPLC-PDA-ESI-QqQ of Buddleja scordiodes plants elicited with salicylic acid. J Chem. https://doi.org/10.1155/2018/4536970 CrossRefGoogle Scholar
- Díaz-Rivas JO, Herrera-Carrera E, Gallegos-Infante JA, Rocha-Guzmán NE, González-Laredo RF, Moreno-Jiménez MR, Ramos-Gómez M, Reynoso-Camacho R, Larrosa-Pérez M, Gallegos-Corona MA (2015) Gastroprotective potential of Buddleja scordioides Kunth Scrophulariaceae infusions; effects into the modulation of antioxidant enzymes and inflammation markers in an in vivo model. J Ethnopharmacol 169:280–286CrossRefGoogle Scholar
- Potapovich AI, Lulli D, Fidanza P, Kostyuk VA, De Luca C, Pastore S, Korkina LG (2011) Plant polyphenols differentially modulate inflammatory responses of human keratinocytes by interfering with activation of transcription factors NFkB and AhR and EGFR-ERK pathway. Toxicol Appl Pharmacol 255:138–149CrossRefGoogle Scholar
- Rocha-Guzmán NE, Simental-Mendía LE, Barragán-Zúñiga LJ, Ramírez-España JC, Gallegos-Infante JA, Luján-Mendoza CI, Gamboa-Gómez CI (2018) Effect of Buddleja scordiodes K. leaves infusion on lipid peroxidation in mice with ultraviolet light-induced oxidative stress. Med Chem Res 27:2379–2385CrossRefGoogle Scholar
- Yang Y, Bae WK, Nam S-J, Heong M-H, Zhou R, Park S-Y, Tas I, Hwang Y-H, Park M-S, Chung IJ, Kim KK, Hur J-S, Kim H (2018) Acetonic extracts of the endolichenic fungus EL002332 isolated from Endocarpon pusillum exhibits anticancer activity in human gastric cancer cells. Phytomedicine 40:106–115CrossRefGoogle Scholar