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Medicinal Chemistry Research

, Volume 28, Issue 12, pp 2218–2231 | Cite as

Acetone effects on Buddleja scordioides polyphenol extraction process and assessment of their cellular antioxidant capacity and anti-inflammatory activity

  • Cecilia Villegas-Novoa
  • José A. Gallegos-Infante
  • Rubén F. González-Laredo
  • Alejandro M. García-Carrancá
  • Karen M. Herrera-Rocha
  • Janett S. Jacobo-Karam
  • Martha R. Moreno-JiménezEmail author
  • Nuria E. Rocha-GuzmánEmail author
Original Research

Abstract

The use of water, acetone, and hydroacetonic solutions as an extraction solvent for polyphenol compounds from Buddleja scordioides has been investigated. The phenolic profiles determined by liquid chromatography—electrospray ionization—mass spectrometry/mass spectrometry (LC–ESI–MS/MS) were compared with those obtained with the traditional aqueous infusion used for the treatment of gastrointestinal disorders. It was evaluated the effects of polyphenol extract at physiologically relevant conditions against H2O2-induced oxidative stress via attenuation of reactive oxygen species (ROS) levels and lipopolysaccharide (LPS)-induced inflammation in human HT-29 cells. The use of hydroacetonic solutions allows obtaining phytochemical extracts enriched with constituents that have antioxidant and anti-inflammatory activity in intestinal cells. This suggests that polyphenols of low and middle polarity from Buddleja scordioides may be better extracted with hydroacetonic solutions, and showing high nutraceutical potential to reduce oxidative stress associated with the onset and progress of inflammatory diseases.

Keywords

Buddleja scordioides Polyphenols Antioxidant Anti-inflammatory activity 

Notes

Acknowledgements

Funding from the Strengthening and Development of Scientific and Technological Infrastructure program (Grants numbers 253333, 280172, and 224651) from the Mexican Science and Technology Council (CONACyT) is recognized. Financial support from TecNM/Instituto Tecnológico de Durango (Grant No. 5557.15) is also acknowledged. Authors CV-N and KMH-R are thankful for graduate scholarships granted by CONACyT for PhD studies.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Cecilia Villegas-Novoa
    • 1
  • José A. Gallegos-Infante
    • 1
  • Rubén F. González-Laredo
    • 1
  • Alejandro M. García-Carrancá
    • 2
  • Karen M. Herrera-Rocha
    • 1
  • Janett S. Jacobo-Karam
    • 3
  • Martha R. Moreno-Jiménez
    • 1
    Email author
  • Nuria E. Rocha-Guzmán
    • 1
    Email author
  1. 1.Research Group on Functional Foods and Nutraceuticals. Departamento de Ingenierías Química y BioquímicaTecNM/Instituto Tecnológico de DurangoDurangoMéxico
  2. 2.Laboratorio de Virus y Cáncer, Unidad de Investigación Biomédica en Cáncer, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de MéxicoInstituto Nacional de Cancerología, Secretaría de SaludDel TlalpanMéxico
  3. 3.Facultad de Medicina y NutriciónUniversidad Juárez del Estado de DurangoDurangoMéxico

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