Medicinal Chemistry Research

, Volume 28, Issue 8, pp 1284–1297 | Cite as

Design, synthesis, biological evaluation and molecular modeling of new coumarin derivatives as potent anticancer agents

  • Eman A. FayedEmail author
  • Rehab Sabour
  • Marwa F. HarrasEmail author
  • Ahmed B. M. Mehany
Original Research


A novel series of coumarin-pyridine/fused pyridine hybrids were designed and synthesized. Their anticancer activity was evaluated against human cancer cell lines MCF-7, HCT-116, HepG-2, and A549. Compounds 9, 10, and 11 showed the most potent growth inhibitory activities with IC50 values ranging from 1.1 to 2.4 μM, against MCF-7 cell line. Flow cytometric analysis revealed that these compounds induced cell cycle arrest in the G2/M phase followed by apoptotic cell death. Consistent with these results, the activity of caspase-3 in MCF-7 cells was tested. The results indicated that compounds 9, 10, and 11 increased caspase-3 activity significantly compared to control group. Moreover, their binding affinity for caspase-3 was confirmed by docking study. Taking all these data together, it is suggested that these coumarin derivatives may be potential antiproliferative agents.

One-pot synthesis of new coumarin derivatives: design, synthesis, molecular modeling and biological evaluation as potent anticancer agents. New coumarin hybrids were evaluated as antiproliferative agents, compounds 9, 10 and 11 induced G2/M arrest and apoptosis through stimulation of caspase-3.


Coumarin-pyridine Docking Anticancer Cell cycle and caspase-3 enzyme 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

44_2019_2373_MOESM1_ESM.pdf (531 kb)
Supplementary Information


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Pharmaceutical Organic Chemistry Department, Faculty of Pharmacy (Girls)Al-Azhar UniversityCairoEgypt
  2. 2.Pharmaceutical Chemistry Department, Faculty of PharmacyAl-Azhar University (Girls)CairoEgypt
  3. 3.Department of Zoology, Faculty of Science (Boys)Al-Azhar UniversityCairoEgypt

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