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Medicinal Chemistry Research

, Volume 28, Issue 5, pp 646–656 | Cite as

Anchorage of curcumin onto PVP enhances anti-tumor effect of curcumin

  • Lifang Guo
  • Mingbiao Shi
  • Nan Song
  • Zirui Wan
  • He LiuEmail author
  • Lihong LiuEmail author
Original Research
  • 33 Downloads

Abstract

Curcumin, a natural polyphenol compound, exhibits wide range of pharmacological activities. However, its poor aqueous solubility, weak stability, and rapid metabolism limit its potential development. In this study, solid dispersion of curcumin with PVP was developed. This study aimed to evaluate the loading capacity, stability, solubility, and anti-tumor activity of PVP-Cur solid dispersion. Scanning electron microscope (SEM), differential scanning calorimetry (DSC), X-ray powder diffraction (XRPD), and fourier transform infrared (FTIR) spectroscopy were used to investigate physical characterization of PVP-Cur. Loading capacity, stability, and solubility of PVP-Cur were evaluated by spectrophotometer. The biological effects of PVP-Cur on tumor cell proliferation and apoptosis were analyzed using CCK-8 assay and 7-AAD/AnnexinV-APC assay, respectively. PVP-cur-induced activation of caspase-3 and caspase-9 were evaluated by in vitro caspase activity assay and western blotting. It was interesting to note that PVP: curcumin in the ratios of 6:1 or 8:1 exhibited the highest loading capacity. Anchorage of curcumin onto PVP increased its solubility and stability. Compared with curcumin alone, PVP-Cur displayed an increased ability in abrogating tumor cell proliferation and inducing tumor cell apoptosis via mitochondria signaling, which was attributed by the enhanced cellular uptake of PVP-Cur. Anchorage of curcumin onto PVP was an effective way to increase the solubility and stability of curcumin. PVP-Cur increased anti-tumor ability and cellular uptake relative to curcumin, indicating that PVP-Cur is a promising candidate for anti-cancer drug development.

Keywords

Curcumin Polyvinyl pyrrolidone Solubility PVP-Cur 

Notes

Acknowledgements

This work was supported by the Natural Science Foundation of China (No. 81500336), Beijing Natural Science Foundation (No. 7151004, 7174307), and The China Postdoctoral Science Foundation (Special Fund 2015T81096).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Pharmacy Department of Beijing Chao-Yang HospitalCapital Medical UniversityBeijingP. R. China
  2. 2.Central Hospital of Jiaozuo coal industry Refco Group LtdJiaozuoP. R. China
  3. 3.Beijing Institute of Tropical Medicine, Beijing Friendship HospitalCapital Medical UniversityBeijingP. R. China

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