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A new triterpene diglycoside from the roots of Bupleurum chinense DC. and its inhibitory effect on adipogensis in 3T3-L1 cells

  • Yujing Feng
  • Zhou-Wei Wu
  • Yanyan Luo
  • Liang Chen
  • Yufeng Cao
  • Lun Wang
  • Aftab Yaseen
  • Bin Chen
  • Ashfaq Ahmad Khan
  • Ming-Kui Wang
  • Guo-Lin Zhang
  • Xin-Feng WangEmail author
  • Fu LiEmail author
  • Xueqin Li
  • Weicheng HuEmail author
Original Research
  • 34 Downloads

Abstract

A new triterpene diglycoside, tibesaikosaponin V (TKV), was isolated from the methanol extract of the roots of Bupleurum chinense DC. by normal and reversed phase column chromatography. The chemical structure of this new compound was elucidated based on extensive spectroscopic analysis, including 1D and 2D NMR, HR-ESI-MS, and chemical degradation method. Our results indicated that TKV inhibited lipid accumulation and triacylglycerol content occurred without cytotoxicity to 3T3-L1 adipocytes. Furthermore, TKV significantly suppressed the mRNA expression of nuclear transcription factors such as, peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α (C/EBPα). These results suggest that TKV shows the ability to inhibit 3T3-L1 preadipocyte differentiation of and may have therapeutic potential for obesity and its associated metabolic disorders.

Keywords

Bupleurum chinense DC. Triterpene diglycoside Tibesaikosaponin V 

Notes

Acknowledgements

This work was financially supported by the West Light Foundation of Chinese Academy of Sciences (Y7C1031100), National Natural Science Foundation of China (21561142003, 31600281 and 41501262) Natural Science Foundation of Jiangsu Province (BK20171269), and Qing Lan Project of Jiangsu Province.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

44_2018_2279_MOESM1_ESM.docx (208 kb)
Supplementary Materials

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze LakeHuaiyin Normal UniversityHuaianChina
  2. 2.Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of BiologyChinese Academy of SciencesChengduChina
  3. 3.Department of ChemistryWomen University of Azad Jammu and KashmirBaghPakistan
  4. 4.Department of GerontologyThe Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityHuaianChina

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