Isolation of three new metabolites and intervention of diazomethane led to separation of compound 1 & 2 from an endophytic fungus, Cryptosporiopsis sp. depicting cytotoxic activity
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The discovery of three new natural products (1, 4, and 5), one semi-synthetic derivative (3) along with two known compounds (2 and 6) were isolated from an endophytic fungus Cryptosporiopsis sp. The structural elucidations of 1–6 were authenticated by one-dimensional and two-dimensional nuclear magnetic resonance, mass spectroscopy, and X-ray diffraction analysis. Herein, we intervention of diazomethane as tool that help in the crystallization and isolation of inseparable mixtures of compounds 1 and 2. Compounds (1–6) were screened for cytotoxic activity against six cancer cell lines in which the 4-epi-ethisolide (2) exhibited moderate activity with IC50 values 11 µM in HL-60, whereas the compound 3 lost its cytotoxic potentiality, but it displayed moderate antimicrobial activity. The result illustrates that the methylene moiety in 2 plays significant role in cytotoxic potential.
KeywordsEndophytic fungus Cryptosporiopsis sp. Cytotoxicity Chemical engineering
The authors are grateful to the CSIR-New Delhi for providing financial support (project no. P81101) for the work of SK. He also acknowledges the AcSIR for their enrolment in Ph. D program. We acknowledge to D. Singh for running and processing NMR experiments. We kindly acknowledge Department of Science & Technology for single crystal X-ray diffractometer as a National Facility under Project No. SR/S2/CMP-47/2003. The manuscript bears the institutional publication no. IIIM/1777/2015.
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Conflict of interest
The authors declare that they have no competing interests.
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