Involvement of reactive oxygen species in the oleoylethanolamide effects and its pyrazonilic analogue in melanoma cells
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The search for more substances that effectively fight melanoma is extremely important, because of its aggressive nature. In this sense, the molecular hybridization is a promising strategy. The aim of this work is to evaluate whether the antiproliferative effect of the endocannabinoid oleoylethanolamide can be improved with the addition of a trifluoromethylated pyrazolinic nucleus on its structure in B16F10 cell line. The pyrazolinic analog was named oleoyl pyrazoline. We also compared the effects of oleoylethanolamide and that of the classic endocannabinoid anandamide (AEA). The cell viability was evaluated by MTT assay, the intracellular reactive oxygen species generation by fluorimetry, and apoptosis/necrosis by fluorescent microscopy. Also, α-tocopherol antioxidant was used to evaluate the involvement of reactive oxygen species in the cellular response. Although the effects of AEA occur in smaller concentrations, the results show that the effects of AEA and oleoylethanolamide were similar. The results showed a decrease in cell viability, induction of apoptosis and necrosis, and increased generation of reactive oxygen species by the oleoylethanolamide, while the oleoyl pyrazoline increased cell proliferation and decreased reactive oxygen species. Additionally, the effects of oleoylethanolamide in cell viability were decreased by inhibiting the generation of reactive oxygen species by α-tocopherol. Therefore, it is possible to suggest the involvement of reactive oxygen species in the effect of oleoylethanolamide in the B16F10 cells. Considering the great need to find substances that can fight melanoma and the lack of greater elucidation in the action mechanisms of cannabinoids and their analogs, this work provides important new information that could serve as reference to other studies.
KeywordsCell viability Apoptosis B16F10 cell line Oleoylpyrazoline Oxidative stress
This work was supported by the Programa de Pós-Graduação em Ciências Fisiológicas (FURG). P.A.S. received a graduate fellowship from Brazilian CAPES. We are thankful to Anahy Fazio, Milene Medeiros and Fernanda Lopes for help on the work, and for financial support from Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS/PRONEM) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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Conflicts of interest
The authors declare that they have no competing interests.
- American Cancer Society (2016) Cancer Facts and Figures. http://www.cancer.org/acs/groups/content/@research/documents/document/acspc-047079.pdf. Accessed 5 May 2017
- Carracedo A, Lorente M, Egia A, Blazquez C, Garcia S, Giroux V, Malicet C, Villuendas R, Gironella M, Gonzáles-Feria L, Piris MA, Iovanna JL, Guzmán M, Velasco G (2006) The stress-regulated protein p8 mediates cannabinoid-induced apoptosis of tumor cells. Cancer Cell 9:301–312CrossRefPubMedGoogle Scholar
- Mechoulam R, Ben-Shabat S, Hanus L, Ligumsky M, Kaminski NE, Schatz AR, Gopher A, Almog S, Martin BR, Compton DR, Pertwee RG, Griffin G, Bayewitch M, Barg J, Vogel Z (1995) Identification of an endogenous 2-monoglyceride, present in canine gut, that binds to cannabinoid receptors. Biochem Pharmacol 50:83–90CrossRefPubMedGoogle Scholar
- Mechoulam R, Spatz M, Shohami E (2002) Endocannabinoids and neuroprotection. Sci, STKE 23:129–re5Google Scholar
- Nenajdenko VG, Balenkova ES (2011) Preparation of unsaturated trifluoromethylketones and their application in the synthesis of heterocycles. ARKIVOC 1:246–328Google Scholar
- Sauzem PD, Machado P, Rubin MA, Sant’anna GS, Faber HB, Souza AH, Mello CF, Beck P, Burrow RA, Bonacorso HG, Zanatta N, Martins MAP (2008) Design and microwave-assisted synthesis of5-trifluoromethyl-4,5-dihydro-1H-pyrazoles: novel agents with analgesic and anti-inflammatory properties. Eur J Med Chem 43:1237–1247CrossRefPubMedGoogle Scholar
- Treptow TGM, Figueiró F, Jandrey EHF, Battastini AMO, Salbego CG, Hoppe JB, Taborda PS, Rosa SB, Piovesan LA, Montes D’Oca CR, Russowsky D, Montes D’Oca MG (2015) Novel hybrid DHPM-fatty acids: synthesis and activity against glioma cell growth in vitro. Eur J Med Chem 95:552–562CrossRefPubMedGoogle Scholar