Medicinal Chemistry Research

, Volume 26, Issue 11, pp 2699–2706 | Cite as

Towards the design of 3-aminopyrazole pharmacophore of pyrazolopyridine derivatives as novel antioxidants

  • Apilak WorachartcheewanEmail author
  • Chanin Nantasenamat
  • Supaluk Prachayasittikul
  • Anyaporn Aiemsaard
  • Virapong PrachayasittikulEmail author
Original Research


Free radicals and oxidants can cause oxidative damage to physiologically important biomolecules that subsequently leads to the development of a wide range of chronic and degenerative diseases such as aging, cancer, cardiovascular and neurodegenerative diseases. Antioxidants have been shown to be instrumental in counteracting the deleterious effects of these reactive oxygen species. Herein, a series of 20 pyrazolopyridine derivatives with antioxidant activity were utilized for constructing a quantitative structure–activity relationship model as to unravel the origins of the antioxidant activity. Quantum chemical and molecular descriptors were used to quantitate the physicochemical properties of investigated compounds. Significant descriptors as identified by stepwise regression analysis consisted of Mor11m, Mor25v, JGI5, H8p, GATS5p, and GVWAI-50. Statistical parameters suggested that the constructed quantitative structure–activity relationship models were robust with Q 2 = 0.9370 and root mean square error  = 4.7414 as evaluated via leave-one-out cross-validation. The mechanistic basis of the antioxidant activity as deduced from significant descriptors was rationalized. Particularly, compounds with the highest antioxidant activity required compounds to have the highest mean topological charge index of order 5 (JGI5) and Geary autocorrelation–lag 5/weighted by atomic polarizabilities (GATS5p) but necessitated low 3D-MoRSE-signal 25/weighted by atomic van der Waals volumes (Mor25v). Such properties are well corroborated by the 3-aminopyrazole pharmacophore from investigated compounds. Molecular insights unraveled herein is anticipated to be useful as guidelines for further rational design of novel pyrazole analogs with potent antioxidant activity.


Pyrazolopyridine Antioxidant activity Oxidative stress QSAR Multiple linear regression Data mining 



We gratefully acknowledge the support from the Office of the Higher Education Commission, Mahidol University under the National Research Universities Initiative as well as partial support from the Annual Government Grant under Mahidol University (B.E. 2556–2558). AW gratefully acknowledges the research grant supported by the Thailand Research Fund (Grant No. TRG5880143).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Apilak Worachartcheewan
    • 1
    • 2
    • 3
    Email author
  • Chanin Nantasenamat
    • 3
  • Supaluk Prachayasittikul
    • 3
  • Anyaporn Aiemsaard
    • 1
  • Virapong Prachayasittikul
    • 4
    Email author
  1. 1.Department of Community Medical Technology, Faculty of Medical TechnologyMahidol UniversityBangkokThailand
  2. 2.Department of Clinical Chemistry, Faculty of Medical TechnologyMahidol UniversityBangkokThailand
  3. 3.Center of Data Mining and Biomedical Informatics, Faculty of Medical TechnologyMahidol UniversityBangkokThailand
  4. 4.Department of Clinical Microbiology and Applied Technology, Faculty of Medical TechnologyMahidol UniversityBangkokThailand

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