Medicinal Chemistry Research

, Volume 23, Issue 1, pp 382–387 | Cite as

A potent cytotoxic metabolite from terrestrial actinomycete, Streptomyces collinus

  • S. A. Rather
  • Sunil Kumar
  • Bilal Rah
  • Mohammad Arif
  • Asif Ali
  • Parvaiz Qazi
Original Research


In the present study a cytotoxic secondary metabolite, 4-hydroxy-4-(4-nitrophenyl)butan-2-one (compound-1), was isolated from the fermentation broth of the terrestrial actinomycetes. The strain was collected from high altitude regions of Kashmir and it was confirmed as Streptomyces collinus (SRP-19) on the basis of 16S rDNA analysis. To the best of our knowledge the compound-1 has not been reported so far from natural sources. The structure of the isolated compound was established by means of spectroscopic analysis including 1H, 13C, 2D NMR and it was also evaluated for its cytotoxic potential against four cancer cell lines, viz., HeLa, PC-3, THP and Caco-2 cells. Compound 1 exhibited significant cytotoxic activity, having IC50 values within a range of 5.39–8.86 μM.


Actinomycetes Streptomyces collinus (SRP-19) 16S rDNA Cytotoxic 



The authors would like to thank Dr. R. A. Vishwakarma (DIRECTOR), Indian Institute of Integrative Medicine-Jammu (India) for providing the necessary facilities to carry out the research work and the Council of Scientific and Industrial Research (CSIR), India for financial support.

Supplementary material

44_2013_640_MOESM1_ESM.docx (2.2 mb)
Supplementary material 1 (DOCX 2217 kb)


  1. Agarwal R, D’Souza T, Morin PJ (2005) Claudin-3 and claudin-4 expression in ovarian epithelial cells enhances invasion and is associated with increased matrix metalloproteinase-2 activity. Cancer Res 65:7378–7385CrossRefPubMedGoogle Scholar
  2. Balagurunathan R, Radhakrishnan M (2007) Actinomycetes: diversity and their importance. In: Trivedi PC (ed) Applied microbiology: applications and current trends. Pointer, New Delhi, pp 297–329Google Scholar
  3. Baltz RH (2008) Renaissance in antibacterial discovery from actinomycetes. Curr Opin Pharmacol 8:557–563CrossRefPubMedGoogle Scholar
  4. Black RE, Brown KD, Becker S, Yunus M (1982) Longitudinal studies of infectious diseases and physical growth of children in rural Bangladesh. Am J Epidemiol 115:305–314PubMedGoogle Scholar
  5. Butler MS (2004) The role of natural product chemistry in drug discovery. J Nat Prod 67(12):2141–2153CrossRefPubMedGoogle Scholar
  6. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  7. Garg M, Kanojia D, Khosla A, Dudha N, Sati S, Chaurasiya D, Jagadish N, Seth A, Kumar R, Gupta S, Gupta A, Lohiya NK, Suri A (2008) Sperm-associated antigen 9 is associated with tumor growth, migration, and invasion in renal cell carcinoma. Cancer Res 68:8240–8248CrossRefPubMedGoogle Scholar
  8. Guo HM, Cun LF, Gong LZ, Mi AQ, Jiang YZ (2005) Asymmetric direct aldol reaction catalyzed by an L-prolinamide derivative: considerable improvement of the catalytic efficiency in the ionic liquid. Chem Commun 11:1450–1452CrossRefGoogle Scholar
  9. Holeiter G, Heering J, Erlmann P, Schmid S, Jahne R, Olayioye MA (2008) Deleted in liver cancer 1 controls cell migration through a dia1-dependent signaling pathway. Cancer Res 68:8743–8751CrossRefPubMedGoogle Scholar
  10. Koppikar SJ, Choudhari AS, Suryavanshi SA, Kumari S, Chattopadhyay S, Kaul-Ghanekar R (2010) Aqueous cinnamon extract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential. BMC Cancer 10:210–221PubMedCentralCrossRefPubMedGoogle Scholar
  11. Kuster E, Williams ST (1964) Selection of media for isolation of Streptomycetes. Nature 202:928–929CrossRefGoogle Scholar
  12. Lotan R, Lotan D, Raz A (1985) Inhibition of tumor cell colony formation in culture by a monoclonal antibody to endogenous lectins. Cancer Res 45:4349–4353PubMedGoogle Scholar
  13. Miyadoh S (1993) Research on antibiotic screening in Japan over the last decade: a producing microorganisms approach. Actinomycetologica 7:100–106CrossRefGoogle Scholar
  14. Moncheva P, Tishkov S, Dimitrova N, Chipeva V, Nikolova SA, Bogatzevska N (2000–2002) Characteristics of soil actinomycetes from Antarctica. J Cult Collect 3:3–14Google Scholar
  15. Newman DJ, Cragg GM (2012) Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod 75:311–335PubMedCentralCrossRefPubMedGoogle Scholar
  16. Takahashi Y, Omura S (2003) Isolation of new actinomycete strains for the screening of new bioactive compounds. J Gen App Microbiol 49:141–154CrossRefGoogle Scholar
  17. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Bio and Evol 24:1596–1599CrossRefGoogle Scholar
  18. Tanaka Y, Omura S (1990) Metabolism and products of actinomycetes—an introduction. Actinomycetologica 4:13–14CrossRefGoogle Scholar
  19. Walsh C (2003) Where will new antibiotics come from? Nat Rev Microbiol 1:65–70CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Natural Products Chemistry DivisionIndian Institute of Integrative MedicineJammuIndia
  2. 2.Cancer Pharmacology DivisionIndian Institute of Integrative MedicineJammuIndia
  3. 3.Microbial Biotechnology DivisionIndian Institute of Integrative MedicineSrinagarIndia

Personalised recommendations