A potent cytotoxic metabolite from terrestrial actinomycete, Streptomyces collinus
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In the present study a cytotoxic secondary metabolite, 4-hydroxy-4-(4-nitrophenyl)butan-2-one (compound-1), was isolated from the fermentation broth of the terrestrial actinomycetes. The strain was collected from high altitude regions of Kashmir and it was confirmed as Streptomyces collinus (SRP-19) on the basis of 16S rDNA analysis. To the best of our knowledge the compound-1 has not been reported so far from natural sources. The structure of the isolated compound was established by means of spectroscopic analysis including 1H, 13C, 2D NMR and it was also evaluated for its cytotoxic potential against four cancer cell lines, viz., HeLa, PC-3, THP and Caco-2 cells. Compound 1 exhibited significant cytotoxic activity, having IC50 values within a range of 5.39–8.86 μM.
KeywordsActinomycetes Streptomyces collinus (SRP-19) 16S rDNA Cytotoxic
The authors would like to thank Dr. R. A. Vishwakarma (DIRECTOR), Indian Institute of Integrative Medicine-Jammu (India) for providing the necessary facilities to carry out the research work and the Council of Scientific and Industrial Research (CSIR), India for financial support.
- Balagurunathan R, Radhakrishnan M (2007) Actinomycetes: diversity and their importance. In: Trivedi PC (ed) Applied microbiology: applications and current trends. Pointer, New Delhi, pp 297–329Google Scholar
- Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
- Koppikar SJ, Choudhari AS, Suryavanshi SA, Kumari S, Chattopadhyay S, Kaul-Ghanekar R (2010) Aqueous cinnamon extract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential. BMC Cancer 10:210–221PubMedCentralCrossRefPubMedGoogle Scholar
- Moncheva P, Tishkov S, Dimitrova N, Chipeva V, Nikolova SA, Bogatzevska N (2000–2002) Characteristics of soil actinomycetes from Antarctica. J Cult Collect 3:3–14Google Scholar