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Medicinal Chemistry Research

, Volume 23, Issue 1, pp 382–387 | Cite as

A potent cytotoxic metabolite from terrestrial actinomycete, Streptomyces collinus

  • S. A. Rather
  • Sunil Kumar
  • Bilal Rah
  • Mohammad Arif
  • Asif Ali
  • Parvaiz Qazi
Original Research

Abstract

In the present study a cytotoxic secondary metabolite, 4-hydroxy-4-(4-nitrophenyl)butan-2-one (compound-1), was isolated from the fermentation broth of the terrestrial actinomycetes. The strain was collected from high altitude regions of Kashmir and it was confirmed as Streptomyces collinus (SRP-19) on the basis of 16S rDNA analysis. To the best of our knowledge the compound-1 has not been reported so far from natural sources. The structure of the isolated compound was established by means of spectroscopic analysis including 1H, 13C, 2D NMR and it was also evaluated for its cytotoxic potential against four cancer cell lines, viz., HeLa, PC-3, THP and Caco-2 cells. Compound 1 exhibited significant cytotoxic activity, having IC50 values within a range of 5.39–8.86 μM.

Keywords

Actinomycetes Streptomyces collinus (SRP-19) 16S rDNA Cytotoxic 

Notes

Acknowledgments

The authors would like to thank Dr. R. A. Vishwakarma (DIRECTOR), Indian Institute of Integrative Medicine-Jammu (India) for providing the necessary facilities to carry out the research work and the Council of Scientific and Industrial Research (CSIR), India for financial support.

Supplementary material

44_2013_640_MOESM1_ESM.docx (2.2 mb)
Supplementary material 1 (DOCX 2217 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Natural Products Chemistry DivisionIndian Institute of Integrative MedicineJammuIndia
  2. 2.Cancer Pharmacology DivisionIndian Institute of Integrative MedicineJammuIndia
  3. 3.Microbial Biotechnology DivisionIndian Institute of Integrative MedicineSrinagarIndia

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