Medicinal Chemistry Research

, Volume 21, Issue 12, pp 4037–4042 | Cite as

Simultaneous determination of lisinopril and H2 antagonists in API, formulations and human serum by using two different HPLC systems

  • Safila NaveedEmail author
  • Najma Sultana
  • M. Saeed Arayne
Original Research


An isocratic reversed phase high-performance liquid chromatographic method has been developed for the simultaneous determination of lisinopril and H2 antagonists (cimetidine, ranitidine, and famotidine) in bulk, dosage formulations, and human serum at 225 nm. Chromatographic separation was achieved using mobile phase, acetonitrile:water (70:25 v/v) adjusted to pH 3.0 via phosphoric acid 85% with flow rate of 1 ml/min at room temperature. Calibration curves were linear over range of 0.7–12.50 μg/ml for H2 antagonists and 2.5–100 μg/ml for lisinopril with a correlation coefficient ± 0.999. Limit of detection and limit of quantitation were in the ranges of 0.07–10.4 ng/ml. Intra- and inter-day precision and accuracy results were 98.0–102%.


Lisinopril Cimetidine Ranitidine Famotidine HPLC 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Faculty of PharmacyJinnah University for WomenKarachiPakistan
  2. 2.United BiotechnologiesKarachiPakistan

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