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Medicinal Chemistry Research

, Volume 21, Issue 9, pp 2443–2448 | Cite as

Simultaneous determination of glipizide and glimepride by Rp-Hplc in dosage formulations and in human serum

  • Najma Sultana
  • M. Saeed Arayne
  • Saeeda Nadir Ali
  • M. Hashim Zuberi
Original Research

Abstract

In this article, simple, liquid chromatographic method has been developed and validated for the determination of glipizide and glimepride in pharmaceutical formulations and in human serum. Chromatographic separation was carried out on a Nucleosil, C18 (10 μm, 25 × 0.46 cm) column using the mobile phase 80:20 methanol:water with pH adjusted to 3.5 at a flow rate 1 ml min−1. Peak intensity of the drugs was recorded at 230 nm with UV detection. The linearity of the method was studied over the concentration range of 0.15–5 μg ml−1 (r = 0.9979) and 0.5–7.5 μg ml−1 (r = 0.9988) for glipizide and glimepride, respectively. Detection and quantitation limits were found to be 20 and 46 ng ml−1 and 70 and 141 ng ml−1 for glipizide and glimepride, respectively. There was no significant interference of extra pharmacopeial ingredients and serum observed in the assay of these two drugs.

Keywords

Glipizide Glimepride and Rp-Hplc 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Najma Sultana
    • 1
  • M. Saeed Arayne
    • 1
  • Saeeda Nadir Ali
    • 2
  • M. Hashim Zuberi
    • 2
  1. 1.Dow University of Health SciencesKarachiPakistan
  2. 2.Department of ChemistryUniversity of KarachiKarachiPakistan

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