Antimycobacterial activity of fractions and isolated compounds from Vetiveria zizanioides
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The essential oil of Vetiveria zizanioides showed significant antimycobacterial activity against the drug-resistant strains of Mycobacterium smegmatis, which on activity guided fractionation afforded four bioactive fractions Vz-2, Vz-7, Vz-8, and Vz-9. Further purification of these bioactive fractions over preparative thin layer chromatographic resulted in the characterization of six compounds: 5, 10-pentadecadiyn-1-ol (1), α-curcumene (3), hydroxy junipene (4), (+) cycloisosativene (5), valencine (6), and selino 3,7 (11)-diene (7). All these compounds showed significant antimycobacterial activity against the drug-resistant strains (MDR-R and MDR-40) of M. smegmatis and their MIC was in the range of 31.25–62.5 μg/ml. These results may be of great help in antimycobacterial drug development from a very common, inexpensive, and non toxic natural product.
KeywordsVetiveria zizanioides Mycobacterium smegmatis Drug-resistant strain Antimycobacterial activity 5,10-Pentadecadiyn-1-ol α-Curcumene Hydroxy junipene
Financial support for this research by the Council of Scientific & Industrial Research (CSIR) is gratefully acknowledged. We also thank all of our colleagues for their excellent assistance.
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