Cytotoxic effect of a polymer–copper(II) complex containing 2,2′-bipyridyl ligand on human lung cancer cells
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The cytotoxic activity of the polymer–copper(II) complex, [Cu(bpy)2(BPEI)]Cl2·4H2O (bpy = 2,2′-bipyridine, BPEI = branched polyethyleneimine) on NCI-H460 human lung cancer cells was determined adopting MTT assay and specific staining techniques. Many of the cultured NCI-H460 cancer cells treated with this complex suffered loss of viability and death mostly through apoptosis and/or necrosis as evidenced by the changes in the nuclear morphology and cytoplasmic features.
KeywordsPolymer–metal complex Copper complex Cytotoxicity Apoptosis Necrosis
The DST-FIST and UGC-SAP support for improvement of infrastructure facility at Animal Science Department of Bharathidasan University, and the UGC-SAP, COSIST, and DST-FIST programmes offered to the School of Chemistry are gratefully acknowledged.
- Kasibhatla H, Finucane D, Brunner T, Wetzel EB, Green DR (2000) Cell: a laboratory manual. Protocol: staining of suspension cells with Hoechst 33258 to detect apoptosis, vol 1. Cold Spring Harbor Laboratory Press, New York, pp. 15.5–15.7Google Scholar
- Putnam D, Kopecek J (1995) Polymer conjugates with anticancer activity. Adv Polym Sci 122:55–123Google Scholar
- Spector DL, Goldman RD, Leinwand LA (1998) Cell: a laboratory manual. culture and biochemical analysis of cells, vol 1. Cold Spring Harbor Laboratory Press, New York, pp 34.1–34.9Google Scholar