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Medicinal Chemistry Research

, Volume 17, Issue 2–7, pp 74–84 | Cite as

Antihyperglycaemic activity of Ceriops tagal in normoglycaemic and streptozotocin-induced diabetic rats

  • Priti Tiwari
  • Akhilesh Kumar Tamrakar
  • Rehan Ahmad
  • Mahendra Nath Srivastava
  • Rajesh Kumar
  • Vijai Lakshmi
  • Arvind Kumar SrivastavaEmail author
Original Research

Abstract

Antihyperglycaemic effect of the crude extract (1) and four fractions (2, 3, 4, and 5) of Ceriops tagal (Perr.) C.B. Rob. (Rhizophoraceae) were evaluated in normal healthy sucrose-loaded and STZ-induced diabetic rats. The crude extract improved the glucose tolerance of the sucrose-loaded rats significantly (p < 0.05) with 33.2% activity, while in STZ-induced diabetic rats the extract caused a fall of 10.9% in hyperglycaemia. All of the four isolated fractions improved the glucose tolerance of normal rats post sucrose load, albeit to varying degree. Fraction 4 exerted the strongest effect of 37.2% (p < 0.05) in sucrose-loaded rats while in STZ-induced diabetic rats it caused a fall of 17% (p < 0.05) in hyperglycaemia. The fasting blood glucose levels of STZ-induced diabetic rats were normalized and the glucose tolerance of these animals was significantly improved after 20 days of daily oral administration of fraction 4 at a 100 mg/kg dose.

Keywords

Antihyperglycaemic activity Diabetes mellitus Glucose tolerance Sucrose-loaded rat model Streptozotocin-induced diabetic rat model 

Abbreviations

C. tagal

Ceriops tagal

SLM

sucrose-loaded model

STZ

streptozotocin

b.w.

body weight

SEM

standard error median

AUC

area under curve

PTP

Protein tyrosine phosphatase

PNPP

para-nitrophenyl phosphate

DTTd

Dithiothreitol

EDTA

ethylene diamine tetra acetic acid

HEPES

(N-[2-Hydroxyethyl] piperazine-N’-[2-ethane-sulfonic acid])

NaOH

sodium hydroxide

Notes

Acknowledgement

One of the authors (Priti Tiwari) is thankful to CSIR, New Delhi for providing financial assistance in the form of a Senior Research Fellowship to carry out this work.

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Copyright information

© Birkha¨user Boston 2007

Authors and Affiliations

  • Priti Tiwari
    • 1
  • Akhilesh Kumar Tamrakar
    • 1
  • Rehan Ahmad
    • 1
  • Mahendra Nath Srivastava
    • 2
  • Rajesh Kumar
    • 3
  • Vijai Lakshmi
    • 3
  • Arvind Kumar Srivastava
    • 1
    Email author
  1. 1.Division of BiochemistryCentral Drug Research InstituteLucknowIndia
  2. 2.Division of BotanyCentral Drug Research InstituteLucknowIndia
  3. 3.Divisions of Medicinal Process ChemistryCentral Drug Research InstituteLucknowIndia

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