Insectes Sociaux

, Volume 65, Issue 1, pp 183–189 | Cite as

Day/night upper thermal limits differ within Ectatomma ruidum ant colonies

  • A. S. Nelson
  • T. Scott
  • M. Barczyk
  • T. P. McGlynn
  • A. Avalos
  • E. Clifton
  • A. Das
  • A. Figueiredo
  • L. L. Figueroa
  • M. Janowiecki
  • S. Pahlke
  • J. D. Rana
  • S. O’Donnell
Short Communication


In the tropics, daily temperature fluctuations can pose physiological challenges for ectothermic organisms, and upper thermal limits may affect foraging activity over the course of the day. Variation in upper thermal limits can occur among and within species, and for social insects such as ants, within colonies. Within colonies, upper thermal limits may differ among individuals or change for an individual throughout the day. Daytime foragers of the Neotropical ant Ectatomma ruidum have higher critical thermal maxima (CTmax) than nocturnal foragers, but whether these differences occur among or within colonies was not previously known. We investigated the potential mechanisms accounting for day/night variation in CTmax of E. ruidum foragers by testing whether CTmax varied among or within colonies or due to individuals within colonies acclimating to changes in temperature over a short time scale (3 h). We found within- but not among-colony differences in CTmax on a diel cycle, and we found no evidence for among- or within-colony partitioning of foraging times by individual workers. Individuals did not acclimate to experimental manipulations of temperature, although additional experiments with more ecologically relevant temperature manipulations are needed to rule out this mechanism. In summary, we have shown that day/night differences in upper thermal limits can occur within ant colonies, but further investigation is needed to elucidate the mechanisms driving this variation.


Diel variation Maximum critical temperature Intra-colony variation Acclimation 



We thank the Organization for Tropical Studies (OTS) and the La Selva Biological Station for facilitating and funding this research. Field supplies and equipment were made available from the Tropical Ecology Mentorship Program, NSF Grant no. OISE-1130156. Research was conducted under a course research permit from the Costa Rican government (MINAET/SINAC). A. Nelson was funded by the OTS, the Ayala School of Biological Sciences at the University of California, Irvine, and the National Science Foundation Graduate Research Fellowship Program under Grant no. DGE-1321846. M. Barczyk received funding from two student organizations, Fundacja Bratniak and Fundusz im. J. Kochanowskiego. M. Janowiecki was supported by the Urban Entomology Endowment at Texas A&M University. A. Figueiredo was supported by funding from the OTS and the Whitney R. Harris World Ecology Center at the University of Missouri St. Louis.


  1. Bestelmeyer BT (2000) The trade-off between thermal tolerance and behavioural dominance in a subtropical South American ant community. J Anim Ecol 69:998–1009CrossRefGoogle Scholar
  2. Bishop TR, Robertson MP, Van Rensburg BJ, Parr CL (2017) Coping with the cold: minimum temperatures and thermal tolerances dominate the ecology of mountain ants. Ecol Entomol 42:105–114CrossRefGoogle Scholar
  3. Cahan SH, Nguyen AD, Stanton-Geddes J, Penick CA, Hernáiz-Hernández Y, DeMarco BB, Gotelli NJ (2017) Modulation of the heat shock response is associated with acclimation to novel temperatures but not adaptation to climatic variation in the ants Aphaenogaster picea and A. rudis. Comp Biochem Physiol A Mol Integr Physiol 204:113–120CrossRefGoogle Scholar
  4. Cerdá X, Retana J, Cros S (1997) Thermal disruption of transitive hierarchies in Mediterranean ant communities. J Anim Ecol 66:363–374CrossRefGoogle Scholar
  5. Cerdá X, Retana J, Manzaneda A (1998) The role of competition by dominants and temperature in the foraging of subordinate species in Mediterranean ant communities. Oecologia 117:404–412CrossRefPubMedGoogle Scholar
  6. Chown SL, Gaston KJ, Robinson D (2004) Macrophysiology: large-scale patterns in physiological traits and their ecological implications. Funct Ecol 18:159–167CrossRefGoogle Scholar
  7. Chown SL, Jumbam KR, Sørensen JG, Terblanche JS (2009) Phenotypic variance, plasticity and heritability estimates of critical thermal limits depend on methodological context. Funct Ecol 23:133–140CrossRefGoogle Scholar
  8. da Rocha HR, Goulden ML, Miller SD, Menton MC, Pinto LDVO, de Freitas HC, e Silva Figueira AM (2004) Seasonality of water and heat fluxes over a tropical forest in eastern Amazonia. Ecol Appl 14:22–32CrossRefGoogle Scholar
  9. Esch C, Jimenez JP, Peretz C, Uno H, O’Donnell S (2017) Thermal tolerances differ between diurnal and nocturnal foragers in the ant Ectatomma ruidum. Insectes Soc 64:439–444CrossRefGoogle Scholar
  10. Fitzpatrick G, Lanan MC, Bronstein JL (2014) Thermal tolerance affects mutualist attendance in an ant-plant protection mutualism. Oecologia 176:129–138CrossRefPubMedPubMedCentralGoogle Scholar
  11. Fraser NHC, Metcalfe NB, Thorpe JE (1993) Temperature-dependent switch between diurnal and nocturnal foraging in salmon. Proc R Soc Lond B Biol Sci 252:135–139CrossRefGoogle Scholar
  12. Ghalambor CK, Huey RB, Martin PR, Tewksbury JJ, Wang G (2006) Are mountain passes higher in the tropics? Janzen’s hypothesis revisited. Integr Comp Biol 46:5–17CrossRefPubMedGoogle Scholar
  13. Guénard B, McGlynn TP (2013) Intraspecific thievery in the ant Ectatomma ruidum is mediated by food availability. Biotropica 45:497–502CrossRefGoogle Scholar
  14. Gunderson AR, Stillman JH (2015) Plasticity in thermal tolerance has limited potential to buffer ectotherms from global warming. Proc R Soc B 282:20150401CrossRefPubMedPubMedCentralGoogle Scholar
  15. Huey RB, Stevenson RD (1979) Integrating thermal physiology and ecology of ectotherms: a discussion of approaches. Am Zool 19:357–366CrossRefGoogle Scholar
  16. Jayatilaka P, Narendra A, Reid SF, Cooper P, Zeil J (2011) Different effects of temperature on foraging activity schedules in sympatric Myrmecia ants. J Exp Biol 214:2730–2738CrossRefPubMedGoogle Scholar
  17. Jumbam KR, Jackson S, Terblanche JS, McGeoch MA, Chown SL (2008) Acclimation effects on critical and lethal thermal limits of workers of the Argentine ant, Linepithema humile. J Insect Physiol 54:1008–1014CrossRefPubMedGoogle Scholar
  18. Kaspari M, Clay NA, Lucas J, Yanoviak SP, Kay A (2015) Thermal adaptation generates a diversity of thermal limits in a rainforest ant community. Glob Change Biol 21:1092–1102CrossRefGoogle Scholar
  19. Klotz JH (1984) Diel differences in foraging in two ant species (Hymenoptera: Formicidae). J Kans Entomol Soc 57:111–118Google Scholar
  20. Kronfeld-Schor N, Dayan T (2003) Partitioning of time as an ecological resource. Annu Rev Ecol Evol Syst 34:153–181CrossRefGoogle Scholar
  21. McGlynn TP, Dunn T, Wayman E, Romero A (2010) A thermophile in the shade: light-directed nest relocation in the Costa Rican ant Ectatomma ruidum. J Trop Ecol 26:559–562CrossRefGoogle Scholar
  22. Narendra A, Reid SF, Hemmi JM (2010) The twilight zone: ambient light levels trigger activity in primitive ants. Proc R Soc Lond B Biol Sci 277:1531–1538CrossRefGoogle Scholar
  23. Nelson AS, Scott T, Barczyk M, McGlynn TP, Avalos A, Clifton E, Das A, Figueiredo A, Figueroa LL, Janowiecki M, Pahlke S, Rana JD, O’Donnell S (2017) Data from: Day/night upper thermal limits differ within Ectatomma ruidum ant colonies. Dryad Digital Repository. doi: 10.5061/dryad.6b6q5 Google Scholar
  24. Oms CS, Cerdá X, Boulay R (2017) Is phenotypic plasticity a key mechanism for responding to thermal stress in ants? Sci Nat 104:42. doi: 10.1007/s00114-017-1464-6 CrossRefGoogle Scholar
  25. Penick CA, Diamond SE, Sanders NJ, Dunn RR (2017) Beyond thermal limits: comprehensive metrics of performance identify key axes of thermal adaptation in ants. Funct Ecol 31:1091–1100CrossRefGoogle Scholar
  26. R Core Team (2016) R: a language and environment for statistical computing. R Foundation for Statistical Computing, ViennaGoogle Scholar
  27. Ribeiro PL, Camacho A, Navas CA (2012) Considerations for assessing maximum critical temperatures in small ectothermic animals: insights from leaf-cutting ants. PLOS One 7:e32083CrossRefPubMedPubMedCentralGoogle Scholar
  28. Sørensen JG, Loeschcke V (2002) Natural adaptation to environmental stress via physiological clock-regulation of stress resistance in Drosophila. Ecol Lett 5:16–19CrossRefGoogle Scholar
  29. Stillman JH (2003) Acclimation capacity underlies susceptibility to climate change. Science 301:65–65CrossRefPubMedGoogle Scholar
  30. Via S, Gomulkiewicz R, De Jong G, Scheiner SM, Schlichting CD, Van Tienderen PH (1995) Adaptive phenotypic plasticity: consensus and controversy. Trends Ecol Evol 10:212–217CrossRefPubMedGoogle Scholar
  31. Wiernasz DC, Hines J, Parker DG, Cole BJ (2008) Mating for variety increases foraging activity in the harvester ant, Pogonomyrmex occidentalis. Mol Ecol 17:1137–1144CrossRefPubMedGoogle Scholar
  32. Willhite C, Cupp PV (1982) Daily rhythms of thermal tolerance in Rana clamitans (Anura: Ranidae) tadpoles. Comp Biochem Physiol A Physiol 72:255–257CrossRefGoogle Scholar
  33. Wilson EO (1990) Success and dominance in ecosystems: the case of the social insects. Ecology Institute, Oldendorf/LuheGoogle Scholar

Copyright information

© International Union for the Study of Social Insects (IUSSI) 2017

Authors and Affiliations

  1. 1.Department of Ecology and Evolutionary BiologyUniversity of California at IrvineIrvineUSA
  2. 2.Department of Biological and Biomedical SciencesWashington University in St. LouisSt. LouisUSA
  3. 3.Institute of Environmental SciencesJagiellonian UniversityKrakówPoland
  4. 4.Department of BiologyCalifornia State UniversityCarsonUSA
  5. 5.Department of EntomologyNatural History Museum of Los Angeles CountyLos AngelesUSA
  6. 6.Carl R. Woese Institute for Genomic BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  7. 7.Department of Ecology and Evolutionary BiologyUniversity of ConnecticutStorrsUSA
  8. 8.Department of ZoologyUniversity of CalcuttaKolkataIndia
  9. 9.Department of BiologyUniversity of Missouri-St. LouisSt. LouisUSA
  10. 10.Department of EntomologyCornell UniversityIthacaUSA
  11. 11.Department of EntomologyTexas A&M UniversityCollege StationUSA
  12. 12.Department of Biological SciencesGeorge Washington UniversityWashington, DCUSA
  13. 13.Department of Biodiversity, Earth and Environmental ScienceDrexel UniversityPhiladelphiaUSA

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