Insectes Sociaux

, Volume 62, Issue 3, pp 357–363 | Cite as

Foundress queen mortality and early colony growth of the leafcutter ant, Atta texana (Formicidae, Hymenoptera)

  • H. E. MartiEmail author
  • A. L. Carlson
  • B. V. Brown
  • U. G. Mueller
Research Article


Nest-founding queens of social insects typically experience high mortality rates. Mortality is particularly severe in leafcutter ants of the fungus-growing ant genus Atta that face the challenge of cultivating a delicate fungus garden in addition to raising brood. We quantified foundress queen survivorship of Atta texana that were collected in northwest Texas and maintained in single-queen laboratory nests, and we tracked the rate of colony growth during the first precarious months of the colony lifecycle. Ninety days post-mating flight, only 16.3 % of 141 of the original queens had survived, and colony growth rates varied markedly across the surviving colonies. Worker production was weakly correlated with fungus garden growth over the course of early colony development. Dead queens became overgrown by the parasitic fungi Fusarium oxysporum (26 % of dead queens) and Aspergillus flavus (34 %), and these fungi are therefore possible causes of queen mortality. The phorid fly Megaselia scalaris emerged from one dead queen, but was unlikely the cause of death. Under natural conditions, intense competition between conspecific colonies can amplify small differences in initial growth rates to generate drastic differences in colony fitness. The observed variation in colony growth rate therefore suggests that colony growth is likely an important target for selection to optimize fitness in Atta texana.


Incipient colony Disease Parasite Fusarium oxysporum Aspergillus flavus Megaselia scalaris 



We are grateful to M. Kardish and Z. Phillips for their assistance in data collection and ant care, and to S. Amador-Vargas, E. Dietrich, C. Fang, R. Ma, L. Meirelles, Z. Phillips, C. Smith and two anonymous reviewers for comments that greatly improved this manuscript. The study was funded by an Integrative Biology Fellowship to HEM, and National Science Foundation awards DEB-0919519 and DEB-1354666 to UGM.


  1. Augustin JO, Santos JFL, Elliot SL (2011) A behavioral repertoire of Atta sexdens (Hymenoptera, Formicidae) queens during the claustral founding and ergonomic stages. Insect Soc 58:197–206CrossRefGoogle Scholar
  2. Autuori M (1942) Contribuição para o conhecimento da saúva (Atta spp. -Hymenoptera -Formicidae) II. O saúveria inicial (Atta sexdens rubropilosa Forel, 1908). Arq Inst Biol 13:67–86Google Scholar
  3. Autuori M (1950) Contribuição para o conhecimento da saúva (Atta spp. -Hymenoptera -Formicidae) V. Numero de formas aladas e redução dos saúverios iniciais (Atta sexdens rubropilosa Forel, 1908). Arq Inst Biol 19:325–331Google Scholar
  4. Borgmeier T (1928) Nota previa sobre alguns phorideos que parasitam formigas cortadeiras dos gêneros Atta e Acromyrmex. Bot Biol 14:119–126Google Scholar
  5. Borgmeier T (1931) Sobre alguns phorideos que parasitam a saúva e outras formigas cortadeiras (Diptera: Phoridae). Arq Inst Biol 4:209–228Google Scholar
  6. Boucias DG, Pendland JC (1998) Principles of insect pathology. Kluwer Academic Publishers, DordrechtCrossRefGoogle Scholar
  7. Brian MV (1965) Social insect populations. Academic Press, New YorkGoogle Scholar
  8. Brown BV (1997) Revision of the Apocephalus attophilus group of ant-decapitating flies (Diptera: Phoridae). Contr Sci 468:1–60Google Scholar
  9. Cahan S, Julian GE (1999) Fitness consequences of cooperative colony founding in the desert leaf-cutter ant Acromyrmex versicolor. Behav Ecol 10:585–591CrossRefGoogle Scholar
  10. Clark RM, Fewell JH (2014) Transitioning from unstable to stable colony growth in the desert leafcutter ant Acromyrmex versicolor. Behav Ecol Sociobiol 68:163–171CrossRefGoogle Scholar
  11. Cole BJ (2009) The ecological setting of social evolution: The demography of ant populations. In: Gadau J, Fewell JH (eds) Organization of insect societies: from genome to sociocomplexity. Harvard University Press, Cambridge, pp 74–104Google Scholar
  12. Currie CR, Mueller UG, Malloch D (1999) The agricultural pathology of ant fungus gardens. Proc Natl Acad Sci 96:7998–8002PubMedCentralPubMedCrossRefGoogle Scholar
  13. Disney RHL (2008) Natural history of the scuttle fly, Megaselia scalaris. Annu Rev Entomol 53:39–60PubMedCrossRefGoogle Scholar
  14. Disney RHL, Elizalde L, Folgarait PJ (2008) New species and records of scuttle flies (Diptera: Phoridae) associated with leaf-cutter ants and army ants (Hymenoptera: Formicidae) in Argentina. Sociobiology 51:95–117Google Scholar
  15. Disney RHL, Elizalde L, Folgarait PJ (2009) New species and new records of scuttle flies (Diptera: Phoridae) that parasitize leaf-cutter and army ants (Hymenoptera: Formicidae). Sociobiol 54:601–632Google Scholar
  16. Fernández-Marín H, Zimmerman JK, Wcislo WT (2004) Ecological traits and evolutionary sequence of nest establishment in fungus-growing ants (Hymenoptera, Formicidae, Attini). Biol J Linnean Soc 81:39–48CrossRefGoogle Scholar
  17. Fowler HG (1987) Colonization patterns of the leaf-cutting ant, Atta bisphaerica Forel: evidence for population regulation. J Appl Ent 104:102–105CrossRefGoogle Scholar
  18. Fowler HG, Robinson SW, Diehl J (1984) Effect of mature colony density on colonization and initial colony survivorship in Atta capiguara, a leaf-cutting ant. Biotropica 16:51–54CrossRefGoogle Scholar
  19. Fowler HG, Pereira-da-Silva V, Forti LC, Saes NB (1986) Population dynamics of leaf-cutting ants: A brief review. In: Lofgren CS, Vander Meer RK (eds) Fire ants and leaf-cutting ants: biology and management (studies in insect biology). Westview Press, Boulder, pp 123–145Google Scholar
  20. Ho EKH, Frederickson ME (2014) Alate susceptibility in ants. Ecol Evol 4:4209–4219PubMedCentralPubMedGoogle Scholar
  21. Hughes WOH, Boomsma JJ (2004) Let your enemy do the work: within-host interactions between two fungal parasites of leaf-cutting ants. Proc R Soc Lond B (Suppl) 3:S104–S106CrossRefGoogle Scholar
  22. Jacoby M (1944) Observações e experiências sôbre Atta sexdens rubropilosa Forel visando facilitar seu combate. Bol Min Agric Rio 12:1–55Google Scholar
  23. Mehdiabadi NJ, Hughes B, Mueller UG (2006) Cooperation, conflict, and coevolution in the attine ant-fungus symbiosis. Behav Ecol 17:291–296CrossRefGoogle Scholar
  24. Meirelles LA, Montoya QV, Solomon SE, Rodrigues A (2015) New light on the systematics of fungi associated with attine ant gardens and the description of Escovopsis kreiselii sp. nov. PLoS One 10:e011206i7CrossRefGoogle Scholar
  25. Mintzer AC (1987) Primary polygyny in the ant Atta texana: number and weight of females and colony foundation success in the laboratory. Insect Soc 34:108–117CrossRefGoogle Scholar
  26. Mintzer A, Vinson SB (1985) Cooperative colony foundation by females of the leafcutting ant Atta texana in the laboratory. J New York Entomol Soc 93:1047–1051Google Scholar
  27. Moser JC (1967) Mating Activities of Atta texana (Hymenoptera, Formicidae). Insect Soc 14:295–312CrossRefGoogle Scholar
  28. Mueller UG, Scott JJ, Ishak HD, Cooper M, Rodrigues A (2010) Monoculture in leafcutter ant gardens. PLoS ONE 5:e12668PubMedCentralPubMedCrossRefGoogle Scholar
  29. Mueller UG, Mikheyev AS, Solomon SE, Cooper M (2011a) Frontier mutualism: co-evolutionary patterns at the northern range limit of the leafcutter ant-fungus symbiosis. Proc R Soc Lond 278:3050–3059CrossRefGoogle Scholar
  30. Mueller UG, Mikheyev AS, Hong E, Sen R, Warren DL, Solomon SE, Ishak HD, Cooper M, Miller JL, Shaffer KA, Juenger TE (2011b) Evolution of cold-tolerant fungal symbionts permits winter fungiculture by leafcutter ants at the northern frontier of a tropical ant-fungus symbiosis. Proc Natl Acad Sci 108:4053–4056PubMedCentralPubMedCrossRefGoogle Scholar
  31. O’Donnell K, Humber RA, Geiser D, Kang S, Park B, Robert V, Crous PW, Johnston PR, Aoki T, Rooney AP, Rehner SA (2012) Phylogenetic diversity of insectivorous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST. Mycology 104:427–445CrossRefGoogle Scholar
  32. Oster GF, Wilson EO (1978) Caste and ecology in the social insects. Princeton University Press, PrincetonGoogle Scholar
  33. Porter SD, Pesquero MA, Campiolo S, Fowler HG (1995) Growth and development of Pseudacteon phorid fly maggots (Diptera: Phoridae) in the heads of Solenopsis fire ant workers (Hymenoptera: Formicidae). Environ Entomol 24:475–479CrossRefGoogle Scholar
  34. R Core Team (2013) R: a language and environment for statistical computing. R Foundation for Statistical Computing, ViennaGoogle Scholar
  35. Rehner SA (2012) Phylogenetic diversity of insectivorous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST. Mycol 104:427–445CrossRefGoogle Scholar
  36. Rissing SW, Pollock GB, Higgins MR, Hagen RH, Smith DR (1989) Foraging specialization without relatedness or dominance among co-founding ant queens. Nature 388:420–422CrossRefGoogle Scholar
  37. Rodrigues A, Pagnocca FC, Bacci M Jr, Hebling MJA, Bueno OC, Pfenning LH (2005) Variability of non-mutualistic filamentous fungi associated with Atta sexdens rubropilosa nests. Folia Microbiol 50:421–442CrossRefGoogle Scholar
  38. Rodrigues A, Bacci M, Mueller UG, Ortiz A, Pagnocca FC (2008) Microfungal “weeds” in the leafcutter ant symbiosis. Microb Ecol 56:604–614PubMedCrossRefGoogle Scholar
  39. Rodrigues A, Silva A, Bacci M, Forti LC, Pagnocca FC (2010) Filamentous fungi found on foundress queens of leaf-cutting ants (Hymenoptera: Formicidae). J Appl Entomol 134:342–345CrossRefGoogle Scholar
  40. Rodrigues A, Mueller UG, Ishak HD, Bacci M Jr, Pagnocca FC (2011a) Ecology of microfungal communities in gardens of fungus-growing ants (Hymenoptera: Formicidae): a year-long survey of three species of attine ants in Central Texas. FEMS Microbiol Ecol 78:244–255PubMedCrossRefGoogle Scholar
  41. Rodrigues A, Silva A, Bacci M Jr, Forti LC, Pagnocca FC (2011b) Filamentous fungi found on foundress queens of the leaf-cutting ants (Hymenoptera: Formicidae). J Appl Entomol 134:342–345CrossRefGoogle Scholar
  42. Seal JN, Mueller UG (2014) Instability of novel ant-fungal associations constrains horizontal exchange of fungal symbionts. Evol Ecol 28:157–176CrossRefGoogle Scholar
  43. Seal JN, Tschinkel WR (2007) Energetics of newly-mated queens and colony founding in the fungus-gardening ants Cyphomyrmex rimosus and Trachymyrmex septentrionalis (Hymenoptera: Formicidae). Physiol Entomol 32:8–15CrossRefGoogle Scholar
  44. Seal JN, Schiott M, Mueller UG (2014) Ant-fungus species combinations engineer physiological activity of fungus gardens. J Exp Biol 217:2540–2547PubMedCrossRefGoogle Scholar
  45. St. Leger RJ, Screen SE, Shams-Pirzadeh B (2000) Lack of host specialization in Aspergillus flavus. Appl Environ Microbiol 66:320–324PubMedCentralPubMedCrossRefGoogle Scholar
  46. Weber NA (1972) Gardening ants, the attines. The American Philosophical Society, PhiladelphiaGoogle Scholar
  47. Wilson EO (1971) The insect societies. Harvard University Press, CambridgeGoogle Scholar
  48. Yek SH, Mueller UG (2011) The metapleural gland of ants. Biol Rev 91:201–224Google Scholar

Copyright information

© International Union for the Study of Social Insects (IUSSI) 2015

Authors and Affiliations

  1. 1.Department of Integrative BiologyUniversity of TexasAustinUSA
  2. 2.Natural History Museum of Los Angeles CountyLos AngelesUSA

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