A behavioral repertoire of Atta sexdens (Hymenoptera, Formicidae) queens during the claustral founding and ergonomic stages


Founding queens are central to the survival and maintenance of social insect colonies but are often ignored in studies of social insects. We expect the behaviors performed by a newly-mated ant queen to be critical to colony survival and maintenance but what are these behaviors and how do they change over time as colonies grow? The aim of this work was to describe and compare the behavioral repertoire performed by newly mated Atta sexdens queens during both founding and ergonomic stages. Using the Focal Animal Sampling method, 42 behavioral acts were identified and grouped into seven categories, according to their probable biological function. The most frequent behavioral acts were those related to selfgrooming, indicating the potential vulnerability of the colony to parasites at this early stage. Contrary to previous reports, A. sexdens queens feed on the fungus garden when founding new colonies, indicating that, although not exogenous, fungal staphylae, together with trophic eggs, supply the founding queens with a ready energy source they need during the founding stage. The behavioral repertoires of the queens that died during the observational period were compared with those of queens that survived. Before dying, queens performed a behavioral repertoire made almost exclusively of stereotyped behaviors, showing no apparent biological function. Queens that died exhibited lower frequencies of selfgrooming and did not ingest any staphylae, while laying more trophic eggs than the queens that survived. Our results indicate that selfgrooming may be a key behavior for queens to succeed in founding a colony in the absence of worker labor. Further, the behavioral repertoire reported can serve as a foundation for future behavioral work on this important phase in the life of social insects.

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  1. Altmann J. 1974. Observational study of behavior: sampling methods. Behaviour 49: 227–267

  2. Augustin J.O. 2007. Sociometria e comportamento de rainhas de saúva (Atta sexdens Linnaeus, 1758) (Hymenoptera: Formicidae) mantidas em laboratório. Dissertação Universidade Federal de Juiz de Fora, Brasil. 75 pp

  3. Augustin J.O. and Santos J.F.L. 2008. Behavior of early generations of Atta sexdens Hymenoptera: Formicidae) workers during preparation of leaf substrate for symbiont fungus gardens. Sociobiology 51: 265–281

  4. Autuori M. 1941. Contribuição para o conhecimento da saúva (Atta spp. - Hymenoptera: Formicidae). I. Evolução do sauveiro (Atta sexdens rubropilosa Forel, 1908). Arq. Inst. Biol. 12: 197–228

  5. Autuori M. 1942. Contribuição para o conhecimento da saúva (Atta spp. - Hymenoptera: Formicidae). II. O sauveiro inicial (Atta sexdens rubropilosa Forel, 1908). Arq. Inst. Biol. 13: 67–86

  6. Baer B., Armitage S.A.O. and Boomsma J.J. 2006. Sperm storage induces an immunity cost in ants. Nature 441: 872–875

  7. Basibuyuk H.H. and Quicke D.L.J. 1999. Grooming behaviours in the Hymenoptera (Insecta): potential phylogenetic significance. Zool. J. Linn. Soc. 125: 349–382

  8. Bass M. and Cherrett J.M. 1994. The role of leaf-cutting ant workers (Hymenoptera: Formicidae) in fungus garden maintenance. Ecol. Entomol. 19: 215–220

  9. Bass M. and Cherrett J.M. 1995. Fungal hyphae as a source of nutrients for the leaf-cutting ant Atta sexdens. Physiol. Entomol. 20: 1–6

  10. Bass M. & Cherrett J.M. 1996. Leaf-cutting ants (Formicidae, Attini) prune their fungus to increase and direct its productivity. Funct. Ecol. 10: 55–61

  11. Boomsma J.J. and Aanen D.K. 2009. Rethinking crop-disease management in fungus-growing ants. Proc.. Natl Acad. Sci. USA 106: 17611–17612

  12. Bot A.N.M., Currie C.R., Hart A.G. and Boomsma J.J. 2001. Waste management in leaf-cutting ants. Ethol. Ecol. Evol. 13: 225–237

  13. Brown M.J.F. and Bonhoeffer S. 2003. On the origin of claustral colony founding in ants. Evol. Ecol. Res. 5: 305–313

  14. Cassill D.L. 2002. Brood care strategies by newly mated monogyne Solenopsis invicta (Hymenoptera: Formicidae) queens during colony founding. Annls Entomol. Soc. Amer. 95: 208–212

  15. Currie C.R. and Stuart A.E. 2001. Weeding grooming of pathogens in agriculture by ants. Proc. R. Soc. London B 268: 1033–1039

  16. Currie C.R., Scott J.A., Summerbell R.C. and Malloch D. 1999. Fungus-growing ants use antibiotic-producing bacteria to control garden parasites. Nature 398: 701–704

  17. Currie C.R., Bot A.N.M. and Boomsma J.J. 2003. Experimental evidence of a tripartite mutualism: bacteria protect ant fungus gardens from specialized parasites. Oikos 101: 91–102

  18. Del-Claro K. 2004. Comportamento Animal - Uma Introdução à Ecologia Comportamental. Livraria e Editora Conceito. 132 pp

  19. Della Lucia T.M.C., Moreira D.D.O., Oliveira M.A.D. and Araújo M.S. 1995. Perdas de pesos de rainhas de Atta durante a fundação e o estabelecimento das colônias. Rev. Bras. Biol. 55: 533–536

  20. Elliot S.L. and Hart A.G. 2010. Density-dependent prophylactic immunity reconsidered in the light of host group-living and social behavior. Ecology 91: 65–72

  21. Fernández-Marín H., Zimmerman J.K. and Wcislo W.T. 2003. Nest-founding in Acromyrmex octospinosus (Hymenoptera, Formicidae, Attini): demography and putative prophylactic behaviors. Insect. Soc. 50: 304–308

  22. Fernández-Marín H., Zimmerman J.K. and Wcislo W.T. 2004. Ecological traits and evolutionary sequence of nest establishment in fungus-growing ants (Hymenoptera, Formicidae, Attini). Biol. J. Linn. Soc. 81: 39–48

  23. Hölldobler B. and Wilson E.O. 1990. The Ants. Belknap Press of Harvard University Press, Cambridge. 732 pp

  24. Johnson R.A. 2006. Capital and incoming breeding and the evolution of colony founding strategies in ants. Insect. Soc. 53: 316–322

  25. Lee K.P., Simpson S.J. and Wilson K. 2008. Dietary protein-quality influences melanization and immune function in an insect. Funct. Ecol. 22: 1052–1061

  26. Little A.E.F. and Currie C.R. 2008. Indirect interaction web reveals how black yeast symbionts compromise the efficiency of antibiotic defenses in fungus-growing ants. Ecology 89: 1216–1222

  27. Mariconi F.A.M. 1970. As Saúvas. Piracicaba, Editora Agronômica Ceres. 167 pp

  28. Mintzer A. 1987. Primary polygyny in the ant Atta texana: number and weight of females and colony foundation success in the laboratory. Insect. Soc. 34: 108–117

  29. Pagnocca F.C, Rodrigues A., Nagamoto N.S. and Bacci M. Jr. 2008. Yeasts and filamentous fungi carried by the gynes of leafcutting ants. Antonie van Leeuwenhoek 94:517–526

  30. Peeters C. and Ito F. 2001. Colony dispersal and the evolution of queen morphology in social Hymenoptera. Annu. Rev. Entomol. 46: 301–330

  31. Porter S.D. and Tschinkel W.R. 1986. Adaptive value of nanitic workers in newly founded red imported fire ant colonies (Hymenoptera: Formicidae). Annls Entomol. Soc. Amer. 79: 723–726

  32. Povey S., Cotter S.C., Simpson S.J., Lee K.P. and Wilson K. 2009. Can the protein costs of bacterial resistance be offset by altered feeding behaviour? J. Anim. Ecol. 78: 437–446

  33. Quinlan R.J. and Cherrett J.M. 1979. The role of fungus in the diet of the leaf-cutting and Atta cephalotes (L.). Ecol. Entomol. 4: 151–160

  34. Rodrigues A., Bacci M., Mueller U.G., Ortiz A. and Pagnocca F.C. 2008. Microfungal ‘‘weeds’’ in the leafcutter ant symbiosis. Microb. Ecol. 56: 604–614

  35. Schultz T.R. and Brady S.G. 2008. Major evolutionary transitions in ant agriculture. Proc. Natl Acad. Sci. USA 105: 5435–5440

  36. Seal J.N. 2009. Scaling of body weight and fat content in fungus-gardening ant queens: does this explain why leaf-cutting ants found claustrally? Insect. Soc. 56: 135–141

  37. Seal J.N. and Tschinkel W.R. 2007. Energetics of newly mated queens and colony founding in the fungus-gardening ants Cyphomyrmex rimosus and Trachymyrmex septentrionalis (Hymenoptera: Formicidae). Physiol. Entomol. 32: 8–15

  38. Seifert K.A., Samson R.A. and Chapela I.H. 1995. Escovopsis aspergilloides, a rediscovered hyphomycete from leaf-cutting ant nests. Mycologia 87: 407–413

  39. Sen R., Ishaka H.D., Estrada D., Dowd S.E., Hong E. and Mueller U.G. 2009. Generalized antifungal activity and 454-screening of Pseudonocardia and Amycolatopsis bacteria in nests of fungus-growing ants. Proc. Natl Acad. Sci. USA 106: 17805–17810

  40. Taerum S.J., Cafaro M. and Currie C.R. 2010. Presence of Multiparasite infections within individual colonies of leaf-cutter ants. Environmental Entomology 1: 105–113

  41. Weber N.A. 1972. Gardening Ants, The Attines. The American Philosophical Society, Philadelphia. 146 pp

  42. Wilson E.O. 1971. The Insects Societies.The Belknap of Harvard University Press. 548 pp

  43. Wilson E.O. 1980. Caste and division of labor in leaf-cutter ants (Hymenoptera: Formicidae: Atta). I. The overall pattern in Atta sexdens. Behav. Ecol. Sociobiol. 7: 143–156

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Appreciation is expressed to Cristiano Augustin for technical support during the completion of this paper, and Ângelo G. Bicalho, Ariane P.P. Melo, Leandro E. Moraes, Mariana S. Brügger and Nádia B. do E. Santo, who helped in the field and laboratory. This research was funded by a grant from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil). We also thank two anonymous referees for their valuable comments on the final version of this manuscript.

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Augustin, J.O., Santos, J.F.L. & Elliot, S.L. A behavioral repertoire of Atta sexdens (Hymenoptera, Formicidae) queens during the claustral founding and ergonomic stages. Insect. Soc. 58, 197–206 (2011) doi:10.1007/s00040-010-0137-7

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  • Attini
  • Claustral founding
  • Colony founding behavior
  • Escovopsis