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Insectes Sociaux

, Volume 50, Issue 2, pp 185–190 | Cite as

Mitochondrial DNA and AFLP markers support species status of Apis nigrocincta

  • D. R. Smith
  • M. R. Palmer
  • G. Otis
  • M. Damus
Research Article

Summary.

New investigations into the biogeography of Asian honey bees are bringing to light previously unrecognized species. On the Indonesian island of Sulawesi, morphometrically distinct populations of Asian cavity-nesting honey bees occur in close sympatry. One population is the widespread species Apis cerana; the other has been proposed as a separate species, A. nigrocincta. A non-coding region of the mitochondrial genome, located between the leucine tRNA and cytochrome oxidase II genes was sequenced in bees from each colony sampled. Consistent sequence differences were noted between the two putative species. Analysis of Molecular Variance (AMOVA) of Amplified Fragment Length Polymorphisms (AFLPs) in each population indicate little or no gene flow between the two, supporting the status of A. nigrocincta as a separate species.

Key words: Apis cerana, Apis nigrocincta, biogeography, AFLP, TE-AFLP, speciation. 

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Copyright information

© Birkhäuser Verlag, 2003

Authors and Affiliations

  • D. R. Smith
    • 1
  • M. R. Palmer
    • 1
  • G. Otis
    • 2
  • M. Damus
    • 3
  1. 1.Department of Ecology and Evolutionary Biology, Entomology Program, Haworth Hall, University of Kansas, Lawrence, Kansas 66045, USA, e-mail: debsmith@ku.eduUS
  2. 2.Department of Environmental Biology, University of Guelph, Guelph, Ontario N1G 2W1, CanadaCA
  3. 3.Natural Resources Canada Strategic Alliances, 580 Booth St., 7th Floor, Ottawa, Ontario K1A OE4, CanadaCA

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