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Alpine Botany

, Volume 129, Issue 1, pp 21–31 | Cite as

Genotyping-by-sequencing (GBS), ITS and cpDNA phylogenies reveal the existence of a distinct Pyrenean/Cantabrian lineage in the European high mountain genus Homogyne (Asteraceae) and imply dual westward migration of the genus

  • Michael A. Gerschwitz-EidtEmail author
  • Joachim W. Kadereit
Original Article

Abstract

Quaternary climatic oscillations have been a major factor in shaping plant diversity and distribution in the European Alpine System (EAS). Plants responded to these oscillations with repeated changes in their abundance and geographical distribution. However, oscillating shifts in geographical distribution have only rarely been reported in molecular analyses of genetic variation across the EAS. Homogyne, a genus endemic to the EAS, contains three species. While H. discolor and H. sylvestris are confined to the periphery of the Eastern Alps, H. alpina is widespread across the EAS. In phylogenetic reconstructions of a broad sample of Homogyne using DNA sequence data sets of the nuclear ribosomal internal transcribed spacer (ITS), plastid DNA (ndhF-rpl32, rpl32-trnL, psbA-trnH) and genotyping-by-sequencing (GBS), accessions of H. alpina from the Pyrenees and the Cantabrian Mts. form a clade which groups in conflicting positions. While the exact relationship of this Pyrenean/Cantabrian clade of H. alpina remains unclear, our data clearly imply that this clade is a lineage distinct from the remaining accessions of H. alpina (H. alpina s.str.). An ancestral area analysis unambiguously revealed the Eastern Alps as the ancestral area of the genus. Considering that relationships within H. alpina s.str. clearly illustrate East to West expansion, the identification of a Pyrenean/Cantabrian clade implies that westward expansion from an ancestral area in the Eastern Alps took place twice in the genus. Although the extant distributions of the Pyrenean/Cantabrian clade and H. alpina s.str. are mutually exclusive, plastid DNA evidence may imply past contact and hybridization between the two clades.

Keywords

Ancestral area reconstruction Extinction Long-distance dispersal Vicariance Repeated range expansion 

Notes

Acknowledgements

We would like to thank Abigail J. Moore (Norman/Oklahoma) and Markus S. Dillenberger (Mainz/Germany) for help with obtaining and analyzing the GBS dataset, M.S. Dillenberger, Simone Steffen (Mainz/Germany), Li-Bing Zhang (St. Louis/Missouri), Hans-Peter Comes (Salzburg/Austria), Christian Uhink (Mainz/Germany) and Matthias Kropf (Vienna/Austria) for collecting material in the field, the curators of BC for permission for using leaf material for DNA analysis, A.J. Moore and H.P. Comes (Salzburg/Austria) as well as two reviewers for very helpful comments on an earlier draft of this manuscript, and Peter Schönswetter (Innsbruck/Austria), Gonzalo Nieto Feliner (Madrid/Spain), Keith D. Bennett (St. Andrews/UK) and Christian Brochmann (Oslo/Norway) for directing us to relevant literature. Financial support by Deutsche Forschungsgemeinschaft (KA 635/8) and Johannes Gutenberg-Universität Mainz is gratefully acknowledged.

Author contributions

JWK designed the research, MAG-E conducted data collection and analyses, and JWK and MAG-E wrote the manuscript.

Funding

This study was funded by grants from the Deutsche Forschungsgemeinschaft (KA 635/8) and Johannes Gutenberg-Universität Mainz to Joachim W. Kadereit.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Data availability

The datasets generated and analyzed during the current study are available in Genbank and the European Nucleotide Archive (accessions see ESM_1) and TreeBASE (http://purl.org/phylo/treebase/phylows/study/TB2:S22621).

Supplementary material

35_2018_212_MOESM1_ESM.docx (26 kb)
Online Resource 1 Taxa used in the analyses, together with sample and voucher information and Genbank and INSDC accession numbers (DOCX 26 KB)
35_2018_212_MOESM2_ESM.docx (94 kb)
Online Resource 2 Phylogenies of Homogyne obtained by maximum parsimony, maximum likelihood and SVDquartets based on genotyping-by-sequencing data. Bootstrap support values are indicated next to the corresponding nodes (DOCX 93 KB)

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Copyright information

© Swiss Botanical Society 2018

Authors and Affiliations

  1. 1.Institut für Organismische und Molekulare EvolutionsbiologieJohannes Gutenberg-Universität MainzMainzGermany

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