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Aquatic Sciences

, Volume 79, Issue 3, pp 487–505 | Cite as

Nitrogen uptake and coupled nitrification–denitrification in riverine sediments with benthic microalgae and rooted macrophytes

  • Erica RacchettiEmail author
  • Daniele Longhi
  • Cristina Ribaudo
  • Elisa Soana
  • Marco Bartoli
Research Article

Abstract

Riverine ecosystems receive nitrogen loads from point and diffuse sources that are transferred downstream. Such loads may undergo poorly explored retention and dissipation processes, varying along gradients of nitrogen availability due to different interactions among primary producers and microbial communities. We measured carbon uptake and nitrogen fluxes in microcosms containing riverine sediments with benthic algae and submerged macrophytes (Vallisneria spiralis L.). Coupled nitrification–denitrification rates were determined via 15N–NH4 + injection in the pore water and quantification of the produced 29N2 and 30N2. Two sites with different N–NO3 concentration and sediment organic content were investigated. We hypothesized that: (1) N–NO3 availability promotes water column N uptake and attenuates primary producers-bacteria competition; (2) coupled nitrification–denitrification is stimulated by radial oxygen loss from roots; (3) macrophyte meadows favour both temporary nitrogen retention and permanent removal. Sediments with V. spiralis were mostly inorganic C and N sinks and always displayed higher coupled nitrification–denitrification rates compared to sediments with microphytobenthos. Highest rates, up to 100 µmol N m−2 h−1, were measured at the more eutrophic site and in the light. This is likely due to a shift from root to leaf uptake at the N–NO3 rich site, attenuating plant-bacteria competition, and to increased radial oxygen loss in organic-rich sediments, stimulating nitrification. High rates of N uptake and loss in lotic sediments represent natural buffers preventing N transport downstream and stress the need to preserve aquatic vegetation and its ecosystem services.

Keywords

Sediment Vallisneria spiralis L. Rhizosphere Microphytobenthos Coupled nitrification–denitrification N-uptake 

Notes

Acknowledgements

E. Racchetti was supported by FLA (Fondazione Lombardia per l’Ambiente, Lombardy Foundation for the Environment).

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Copyright information

© Springer International Publishing 2016

Authors and Affiliations

  • Erica Racchetti
    • 1
    Email author
  • Daniele Longhi
    • 1
  • Cristina Ribaudo
    • 1
    • 2
  • Elisa Soana
    • 1
    • 3
  • Marco Bartoli
    • 1
    • 4
  1. 1.Department of Life SciencesUniversity of ParmaParmaItaly
  2. 2.Irstea-EABXCestasFrance
  3. 3.Department of Life Science and BiotechnologyUniversity of FerraraFerraraItaly
  4. 4.Coastal Research and Planning InstituteMarine Science and Technology Center of Klaipeda UniversityKlaipedaLithuania

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