Aquatic Sciences

, Volume 78, Issue 4, pp 809–822 | Cite as

Compound specific δD and δ13C analyses as a tool for the assessment of hydrological change in a subtropical wetland

  • Ding He
  • William T. Anderson
  • Rudolf Jaffé
Research Article


Compound specific carbon and deuterium stable isotope values (δ13C and δD) and the relative abundance of mid-chain n-alkanes (Paq) were determined for a series of dominant wetland plants, a surface slough-to-ridge soil transect, and slough and ridge soil cores, to assess historical vegetation successions induced by hydrological modification in an anthropogenically impacted, subtropical wetland, the Florida Everglades, USA. A difference of as much as 3.6 and 130 ‰ in their δ13C and δD values was observed between the two most abundant emergent macrophyte species (Cladium and Eleocharis), respectively. A clear n-alkane δD value depletion (−130 to −167 ‰) and decreasing Paq was observed along the slough-to-ridge soil transect, likely the result of an eco-hydrological transition from slough-to-ridge dominated vegetation (Eleocharis to Cladium). In agreement with the relatively constant Paq values, the lack of significant changes in the δD depth profile for the slough core, suggest a consistent slough type of vegetation composition over time at that location. In contrast, changes of both n-alkane δ13C and δD values for the ridge core, especially after ~1960 AD, coincide with the expected plant successions from historically long hydroperiod (>8 months), slough type plants (Eleocharis, Utricularia, Nymphaea) to present day, shorter hydroperiod (<8 months), ridge type plants (Cladium). These δ13C and δD changes seem to be driven by vegetation shifts associated with hydrological change. The application of the compound-specific stable isotope determinations may strongly complement the biomarker approach for paleo-hydrological assessments in wetland ecosystems.


Sub-tropical wetland n-alkanes δD δ13Hydrology 



The authors thank the South Florida Water Management District for logistical support regarding fieldwork. The authors appreciate the assistance of Drs. Colin Saunders and Min Gao for providing the archived samples used in this study. The assistance of John Harris, and the advice from Drs. Julian Sachs and Dirk Sachse during isotope measurements is highly appreciated. This work was funded in part by NSF through the Florida Coastal Everglades LTER program (DEB-1237517) and MRI (OCE 0959722). R.J. and D.H. acknowledge additional support through the George Barley Endowment and the Cristina Menendez Fellowship respectively. This is SERC contribution number 775.

Supplementary material

27_2016_473_MOESM1_ESM.docx (289 kb)
Supplementary material 1 (DOCX 288 kb)


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Copyright information

© Springer International Publishing 2016

Authors and Affiliations

  • Ding He
    • 1
    • 2
  • William T. Anderson
    • 2
    • 3
  • Rudolf Jaffé
    • 1
    • 2
  1. 1.Department of Chemistry and BiochemistryFlorida International UniversityNorth MiamiUSA
  2. 2.Southeast Environmental Research CenterFlorida International UniversityMiamiUSA
  3. 3.Earth and Environment DepartmentFlorida International UniversityMiamiUSA

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