Aquatic Sciences

, Volume 78, Issue 2, pp 343–354 | Cite as

Characterizing organic matter inputs to sediments of small, intermittent, prairie streams: a molecular marker and stable isotope approach

  • Oliva Pisani
  • Walter K. Dodds
  • Rudolf JafféEmail author
Research Article


Small rivers and streams are ecologically important because they contribute to the export of organic carbon to coastal environments, likely influencing the global carbon cycle. While organic matter (OM) dynamics in large rivers has been studied in quite some detail, less is known about small streams. Sources of OM in streams ultimately determine its availability to the food web and downstream transport. In this study, sediment samples were collected from the King’s Creek watershed in Konza Prairie (Kansas, USA) and analyzed using molecular biomarkers and bulk 13C stable isotopes with the objective to comparatively assess OM inputs between riparian forest vegetation and watershed grassland to small, intermittent streams. We are interested in the potential influence of woody riparian expansion that has been ongoing at the site. Biomarkers typical of the local C4 grasses (branched n-alkanes, phytadienes) were more abundant in some of the sediments of the upper reaches. The sediments of the lower reaches contained biomarkers of algae (short-chain aliphatic compounds, C25:5 highly branched isoprenoid, brassicasterol) and vascular plant-derived material (triterpenols). Degraded OM (triterpene/triterpenol ratio) was found throughout the watershed with no pattern between the upper and lower reaches. Bulk 13C isotope analysis showed that the upper reaches of the watershed receive significant OM inputs from the C4 grasses (74–99 %) while the lower reaches are more strongly influenced by riparian trees (26–27 %) and algae (21–22 %). These results suggest that the environmental dynamics of bulk OM and the biomarker composition of small prairie streams are highly complex and likely a function of several factors such as light availability, riparian vegetative composition and density, and varying degrees of OM storage, retention and transport along the river continuum.


Small streams Konza Prairie Riparian Watershed Biomarkers 13C stable isotopes 



This project was a collaborative initiative between the KNZ and FCE LTER (DEB-1237517) programs and funded in part through a supplement to the FCE-LTER. Additional funding was provided through the George Barley endowment to RJ. OP acknowledges the support through the FIU Graduate School for a Dissertation Year Fellowship during this study. We thank two anonymous reviewers and Dr. S. Findley for constructive comments which improved this manuscript. This is contribution number 755 from the Southeast Environmental Research Center at FIU.


  1. Abrams MD (1986) Historical developments of gallery forests in northeast Kansas. Vegetatio 65:29–37CrossRefGoogle Scholar
  2. Allan JD, Castillo MM (1995) Detrital energy sources. Stream ecology: structure and function of running waters, 2nd edn. Springer, The Netherlands, pp 135–160CrossRefGoogle Scholar
  3. Belt ST, Massé G, Allard WG, Robert JM, Rowland SJ (2001) C25 highly branched isoprenoid alkenes in planktonic diatoms of the Pleurosigma genus. Org Geochem 32:1271–1275CrossRefGoogle Scholar
  4. Blair NE, Leithold EL, Ford ST, Peeler KA, Holmes JC, Perkey DW (2003) The persistence of memory: the fate of ancient sedimentary organic carbon in a modern sedimentary system. Geochim Cosmochim Acta 67:63–73CrossRefGoogle Scholar
  5. Brooks PW, Maxwell JR (1974) Early stage fate of phytol in recent deposited lacustrine sediments. In: Tissot B, Bienner F (eds) Advances in organic geochemistry. Editions Technip, Paris, pp 911–977Google Scholar
  6. Cooper JR, Pedentchouk N, Hiscock KM, Disdle P, Krueger T, Rawlins BG (2015) Apportioning sources of organic matter in streambed sediments: an integrated molecular and compound-specific stable isotope approach. Sci Total Environ 520:187–197CrossRefPubMedGoogle Scholar
  7. Cranwell PA (1982) Lipids of aquatic sediments and sedimenting particulates. Prog Lipid Res 21:271–308CrossRefPubMedGoogle Scholar
  8. Ding Y, Yamashita Y, Dodds WK, Jaffé R (2013) Dissolved black carbon in grassland streams: is there an effect of recent fire history? Chemosphere 90:2557–2562CrossRefPubMedGoogle Scholar
  9. Dodds WK, Hutson RE, Eichem AC, Evans MA, Gudder DA, Fritz KM, Gray L (1996) The relationship of floods, drying, flow and light to primary production and producer biomass in a prairie stream. Hydrobiol 333:151–159CrossRefGoogle Scholar
  10. Dodds WK, Evans-White MA, Gerlanc NM, Gray L, Gudder DA, Kemp MJ, Lopez AL, Stagliano D, Strauss EA, Tank JL, Whiles MR, Wollheim WM (2000) Quantification of the nitrogen cycle in a prairie stream. Ecosyst 3:574–589CrossRefGoogle Scholar
  11. Dodds WK, Gido K, Whiles MR, Fritz KM, Matthews WJ (2004) Life on the edge: the ecology of great plains prairie streams. Bioscience 54:205–216CrossRefGoogle Scholar
  12. Dodds WK, Gido K, Whiles MR, Daniels MD, Grudzinski BP (2015) The stream biome gradient concept: factors controlling lotic systems across broad biogeographic scales. Freshwater Sci 34:1–19CrossRefGoogle Scholar
  13. Dornbush ME (2007) Grasses, litter, and their interaction affect microbial biomass and soil enzyme activity. Soil Biol Biochem 39:2241–2249CrossRefGoogle Scholar
  14. Edler C, Dodds WK (1996) The ecology of a subterranean isopod, Caecidotea tridentata. Freshwater Biol 35:249–259CrossRefGoogle Scholar
  15. Eglinton TI (2008) Carbon cycle: tempestuous transport. Nat Geosci 1:727–728CrossRefGoogle Scholar
  16. Eglinton G, Hamilton RJ (1967) Leaf epicuticular waxes. Science 156:1322–1335CrossRefPubMedGoogle Scholar
  17. Farnsworth KL, Milliman JD (2003) Effects of climatic and anthropogenic change on small mountainous rivers: the Salinas River example. Glob Planet Change 39:53–64CrossRefGoogle Scholar
  18. Freeman CC (1998) The flora of Konza Prairie. A historical review and contemporary patterns. In: Knapp AK, Briggs JM, Hartnett DC, Collins SL (eds) Grassland dynamics. Oxford University Press, New York, pp 69–80Google Scholar
  19. Fry B (2006) Stable isotope ecology. Springer, New YorkCrossRefGoogle Scholar
  20. Gallardo A, Merino J (1992) Nitrogen immobilization in leaf litter at two Mediterranean ecosystems of SW Spain. Biogeochem 15:213–228CrossRefGoogle Scholar
  21. Giri SJ, Diefendorf AF, Lowell TV (2015) Origin and sedimentary fate of plant-derived terpenoids in a small river catchment and implications for terpenoids as quantitative paleovegetation proxies. Org Geochem 82:22–32CrossRefGoogle Scholar
  22. Gogou A, Stephanou GE (2004) Marine organic geochemistry of the Eastern Mediterranean: 2. Polar biomarkers in Cretan Sea surficial sediments. Mar Chem 85:1–25CrossRefGoogle Scholar
  23. Grewer DM, Lafrenière MJ, Lamoureux SF, Simpson MJ (2015) Potential shifts in Canadian High Arctic sedimentary organic matter composition with permafrost active layer detachments. Org Geochem 79:1–13CrossRefGoogle Scholar
  24. Harris D, Horwath WR, van Kessel C (2001) Acid fumigation of soils to remove carbonates prior to total organic carbon or carbon-13 isotopic analysis. Soil Soc Am J 65:1853–1856CrossRefGoogle Scholar
  25. Hartmann MA (1998) Plant sterols and the membrane environment. Trends Plant Sci 3:170–175CrossRefGoogle Scholar
  26. He D, Simoneit BRT, Jara B, Jaffé R (2015) Occurrence and distribution of monomethylalkanes in the freshwater wetland ecosystem of the Florida Everglades. Chemosphere 119:258–266CrossRefPubMedGoogle Scholar
  27. Houser JN, Bierman DW, Burdis RM, Soeken-Gittinger LA (2010) Longitudinal trends and discontinuities in nutrients, chlorophyll, and suspended solids in the Upper Mississippi River: implications for transport, processing, and export by large rivers. Hydrobiol 651:127–144CrossRefGoogle Scholar
  28. Huang WY, Meinschen WG (1979) Sterols as ecological indicators. Geochim Cosmochim Acta 43:739–745CrossRefGoogle Scholar
  29. Huang X, Meyers PA, Wu W, Jia C, Xie S (2011) Significance of long chain iso and anteiso monomethyl alkanes in the Lamiaceae (mint family). Org Geochem 42:156–165CrossRefGoogle Scholar
  30. Hwang J, Druffel ERM, Komada T (2005) Transport of organic carbon from the California coast to the slope region: A study of Δ14C and δ13C signatures of organic compound classes. Glob Biogeochem Cycles 19:GB2018. doi: 10.1029/2004GB002422 CrossRefGoogle Scholar
  31. Ikan R, Baedecker MJ, Kaplan IR (1973) C18 isoprenoid ketone in recent marine sediments. Nature 244:154–155CrossRefGoogle Scholar
  32. Jaffé R, Mead R, Hernandez ME, Peralba MC, DiGuida OA (2001) Origin and transport of sedimentary organic matter in two subtropical estuaries: a comparative, biomarker-based study. Org Geochem 32:507–526CrossRefGoogle Scholar
  33. Jaffé R, Yamashita Y, Maie N, Cooper WT, Dittmar T, Dodds WK, Jones JB, Myoshi T, Ortiz-Zayas JR, Podgorski DC, Watanabe A (2012) Dissolved organic matter in headwater streams: compositional variability across climatic regions of North America. Geochim Cosmochim Acta 94:95–108CrossRefGoogle Scholar
  34. Knapp AK, Seastedt TR (1998) Grasslands, Konza Prairie, and long-term ecological research. In: Knapp AK, Briggs JM, Hartnett DC, Collins SL (eds) Grassland dynamics. Oxford University Press, New York, pp 3–15Google Scholar
  35. Komada T, Druffel ERM, Trumbore SE (2004) Oceanic export of relict carbon by small mountainous rivers. Geophys Res Lett 31:L07504. doi: 10.1029/2004GL019512 CrossRefGoogle Scholar
  36. Komada T, Druffel ERM, Hwang J (2005) Sedimentary rocks as sources of ancient organic carbon to the ocean: An investigation through Δ14C and δ13C signatures of organic compound classes. Glob Biogeochem Cycles 19:GB2017. doi: 10.1029/2004GB002347 CrossRefGoogle Scholar
  37. Laws EA, Bidigare RR, Popp BN (1997) Effect of growth rate and CO2 concentration on carbon isotopic fractionation by marine diatom Phaeodactylum tricurnutum. Limnol Oceanogr 42:1552–1560CrossRefGoogle Scholar
  38. Leithold E, Blair NE, Perkey DW (2006) Geomorphologic controls on the age of particulate organic carbon from small mountainous and upland rivers. Glob Biogeochem Cycles 20:GB3022. doi: 10.1029/2005GB002677 CrossRefGoogle Scholar
  39. Marty J, Planas D (2008) Comparison of methods to determine algal δ13C in freshwater. Limnol Oceanogr Methods 6:51–63CrossRefGoogle Scholar
  40. Mead R, Xu Y, Chong J, Jaffé R (2005) Sediment and soil organic matter source assessment as revealed by the molecular distribution and carbon isotopic composition of n-alkanes. Org Geochem 36:363–370CrossRefGoogle Scholar
  41. Medeiros PM, Simoneit BRT (2007) Gas chromatography coupled to mass spectrometry for analyses of organic compounds and biomarkers as tracers for geological, environmental, and forensic research. J Sep Sci 30:1516–1536CrossRefPubMedGoogle Scholar
  42. Medeiros PM, Simoneit BRT (2008) Multi-biomarker characterization of sedimentary organic carbon in small rivers draining the Northwestern United States. Org Geochem 39:52–74CrossRefGoogle Scholar
  43. Medeiros PM, Sikes EL, Thomas B, Freeman KH (2012) Flow discharge influence on input and transport of particulate and sedimentary organic carbon along a small temperate river. Geochim Cosmochim Acta 77:317–334CrossRefGoogle Scholar
  44. Meybeck M, Laroche L, Dürr HH, Syvitski JPM (2003) Global variability of daily total suspended solids and their fluxes in rivers. Glob Planet Change 39:65–93CrossRefGoogle Scholar
  45. Meyers P (1997) Organic geochemical proxies of paleoceanographic, paleolimnologic, and paleoclimatic processes. Org Geochem 27:213–250CrossRefGoogle Scholar
  46. Milliman JD, Syvitski JMP (1992) Geomorphic/tectonic control of sediment transport to the ocean: the importance of small mountainous rivers. J Geol 100:525–544CrossRefGoogle Scholar
  47. Otto A, Simpson MJ (2005) Degradation and preservation of vascular plant-derived biomarkers in grassland and forest soils from Western Canada. Biogeochem 74:377–409CrossRefGoogle Scholar
  48. Peterson BJ, Wollheim WF, Mulholland PJ, Webster JR, Meyer JL, Tank JL, Martí E, Bowden WB, Valett HM, Hershey AE, McDowell WH, Dodds WK, Hamilton SK, Gregory S, Morrall DD (2001) Control of nitrogen export from watersheds by headwater streams. Sci 292:86–90CrossRefGoogle Scholar
  49. Phillips DL, Gregg JW (2003) Source partitioning using stable isotopes: coping with too many sources. Oecol 136:261–269CrossRefGoogle Scholar
  50. Pisani O, Oros DR, Oyo-Ita OE, Ekpo BO, Jaffé R, Simoneit BRT (2013) Biomarkers in surface sediments from the Cross River and estuary system, SE Nigeria: assessment of organic matter sources of natural and anthropogenic origins. Appl Geochem 31:239–250CrossRefGoogle Scholar
  51. Popp BN, Laws EA, Bidigare RR, Dore JE, Hanson KL, Wakeham SG (1998) Effect of phytoplankton cell geometry on carbon isotopic fractionation. Geochim Cosmochim Acta 62:69–77CrossRefGoogle Scholar
  52. Rontani JF, Volkman JK (2003) Phytol degradation products as biogeochemical tracers in aquatic environments. Org Geochem 34:1–35CrossRefGoogle Scholar
  53. Rosemond AD, Benstead JP, Bumpers PM, Gulis V, Kominoski JS, Manning DWP, Suberkropp K, Wallace JB (2015) Experimental nutrient additions accelerate terrestrial carbon loss from stream ecosystems. Sci 347:1142–1145CrossRefGoogle Scholar
  54. Saintilan N, Rogers K (2014) Woody plant encroachment of grasslands: a comparison of terrestrial and wetland settings. New Phytol. doi: 10.1111/nph.13147 Google Scholar
  55. Simoneit BRT (1986) Cyclic terpenoids of the geosphere. In: Johns RB (ed) Biological markers in the sedimentary record. Elsevier, New York, pp 43–99Google Scholar
  56. Simoneit BRT (2005) A review of current applications of mass spectrometry for biomarker/molecular tracer elucidations. Mass Spectrom Rev 24:719–765CrossRefPubMedGoogle Scholar
  57. Smittenberg RH, Pancost RD, Hopmans EC, Paetzel M, Sinninghe Damsté JS (2004) A 400-year record of environmental change in an euxinic fjord as revealed by the sedimentary biomarker record. Palaeogeogr Palaeoclimatol Palaeoecol 202:331–351CrossRefGoogle Scholar
  58. Ten Haven HL, Peakman TM, Rullkötter J (1992) Early diagenetic transformation of higher-plant triterpenoids in deep-sea sediments from Baffin Bay. Geochim Cosmochim Acta 56:2001–2024CrossRefGoogle Scholar
  59. Trudeau V, Rasmussen JB (2003) The effects of water velocity on stable carbon and nitrogen isotope signatures of periphyton. Limnol Oceanogr 48:2194–2199CrossRefGoogle Scholar
  60. Vannote RL, Minshall GW, Cummins KW, Sedell JR, Cushing CE (1980) The river continuum concept. Can J Fish Aquat Sci 37:130–137CrossRefGoogle Scholar
  61. Veach AM, Dodds WK, Skibbe A (2014) Fire and grazing influences on rates of riparian woody plant expansion along grassland streams. PLoS ONE 9:e106922CrossRefPubMedPubMedCentralGoogle Scholar
  62. Volkman JK, Maxwell JR (1986) Acyclic isoprenoids as biological markers. In: Johns RB (ed) Biological markers in the sedimentary record. Elseviere, New York, pp 1–42Google Scholar
  63. Volkman JK, Barrett SM, Blackburn SI, Mansour MP, Sikes EL, Gelin F (1998) Microalgal biomarkers: a review of recent research developments. Org Geochem 29:1163–1179CrossRefGoogle Scholar
  64. Wedin DA, Tieszen LL, Dewey B, Pastor J (1995) Carbon isotope dynamics during grass decomposition and soil organic matter formation. Ecology 76:1383–1392CrossRefGoogle Scholar
  65. Wraige EJ, Belt ST, Lewis CA, Cooke DA, Robert JM, Massé G, Rowland SJ (1997) Variations in structures and distributions of C25 highly branched isoprenoid (HBI) alkenes in cultures of the diatom, Haslea ostrearia (Simonsen). Org Geochem 27:497–505CrossRefGoogle Scholar
  66. Wynn JG, Bird MI (2007) C4-derived soil organic carbon decomposes faster than its C3 counterpart in mixed C3/C4 soils. Glob Change Biol 13:1–12CrossRefGoogle Scholar
  67. Zegouagh Y, Derenne S, Largeau C, Bardoux G, Mariotti A (1998) Organic matter sources and early diagenetic alterations in Arctic surface sediments (Lena River delta and Laptev Sea, Eastern Siberia), II. Molecular and isotopic studies of hydrocarbons. Org Geochem 28:571–583CrossRefGoogle Scholar

Copyright information

© Springer Basel 2015

Authors and Affiliations

  1. 1.Southeast Environmental Research Center and Department of Chemistry & BiochemistryFlorida International UniversityMiamiUSA
  2. 2.Division of BiologyKansas State UniversityManhattanUSA

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