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Aquatic Sciences

, 71:463 | Cite as

Immune deficiency causes annual late summer brown trout (Salmo trutta) mortality in Austrian prealpine river systems

  • Franz LahnsteinerEmail author
  • Reinhard Haunschmid
  • Nabil Mansour
Research Article

Abstract

For several years, severe brown trout (Salmo trutta) mortality has been observed in different prealpine river systems of Austria, Southern Germany, and Switzerland during late summer and early autumn. Often, the skin of the effected fish shows a medium to intense black pigmentation, and therefore this phenomenon is called “Schwarze Bachforelle Phänomen” or “proliferative darkening syndrom”. The present study was conducted to gain insight about its presence. From middle of May to end of August, brown trout and rainbow trout were kept in experimental tanks supplied with water from an effected river system. In brown trout, samples for blood diagnostics and histological investigations were taken after 0, 47, 62, and 77 days, and in rainbow trout after 0 and 107 days. Brown trout from a wild population were investigated for comparative purposes. Brown trout showed no abnormalities till the end of June. All fish sampled in the second half of July suffered from severe immune deficiency as the numbers of peripheral blood granulocytes and lymphocytes, plasma lysozyme activity, and plasma immunoglobulin levels significantly decreased. During August, the number of erythrocytes decreased and the osmotic fragility of the erythrocytes increased. Senescent erythrocytes became more frequent and the hematopoietic tissue of spleen and kidney enlarged. Also, the cellular composition of the spleen changed as the number of hemoblasts and normal erythroblasts decreased while the number of atypical erythroblasts increased. In the pronephros and hematopoietic tissue of the mesonephros, aggregation of macrophages and tissue disintegration was frequently observed. This was similar for brown trout from the field experiment and from the wild population. High mortality occurred in the end of July and during August. Death of the fishes might have been caused by secondary infections with microorganisms. Almost similar changes were observed in rainbow trout, however, the mortality was lower.

Keywords

Brown trout Rainbow trout Mortality Immunity Lymphocytes Granulocytes Immunoglobulin Lysozyme 

Notes

Acknowledgment

The project was funded by the “Oberösterreicher Landesfischereiverband” and by the “Amt der Oberösterreichischen Landesregierung”. We are grateful to Dipl. Ing. Stefan Keil, the head of the sewage plant of Bad Ischl and to his team for the generous support and help in our field studies. Support also came from the fish farm of Kreuzstein and from Mr. Alfred Ungar in providing material.

References

  1. Bly JE, Clem LW (1992) Temperature and teleost immune functions. Fish Shellfish Immunol 2:159–171CrossRefGoogle Scholar
  2. Born O, Schwaiger J (2003) Bachforellensterben in Bayern. Schriftenreihe des Landes-fischeiverbandes Bayern 9Google Scholar
  3. Busse HJ, Glöckner J, Haunschmid R (2005) Identification of bacteria causing bacteremia in brown brout. Congress of Bacteriology and Applied Microbiology, San Francisco, USAGoogle Scholar
  4. Ellis AE (1990) Lysozyme assays. In: van Muiswinkel WB (ed) Techniques in fish immunology. SOS Publications, Fair Haven, pp 101–103Google Scholar
  5. Ellis AE (2001) Innate host defense mechanisms of fish against viruses and bacteria. Dev Comp Immunol 25:827–839CrossRefPubMedGoogle Scholar
  6. Feuerstein G (2004) Bachforellensterben: Allgemeine Betrachtungen zum Problem und neueste Erkenntnisse vom Expositionsversuch in Wartau (SG). http://s.o.w.tripod.com/bachforellensterben.htm
  7. Hadidi S, Glenney GW, Welch TJ, Silverstein JT, Wiens GD (2008) Spleen size predicts resistance of rainbow trout to Flavobacterium psychrophilum challenge. J Immunol 180:4156–4165PubMedGoogle Scholar
  8. Hoeger B, Heuvel MR, van den Hitzfeld BC, Dietrich DR (2004) Effects of treated sewage effluent on immune function in rainbow trout (Oncorhynchus mykiss). Aquat Toxicol 70:345–355CrossRefPubMedGoogle Scholar
  9. Jones SR (2001) The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol 25:841–852CrossRefPubMedGoogle Scholar
  10. Köllner B, Wasserrab B, Kotterba G, Fischer U (2002) Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss)—how can environmental influences be detected? Toxicol Lett 131:83–95CrossRefPubMedGoogle Scholar
  11. Le Morvan CD, Troutaud D, Deschaux P (1998) Differential effects of temperature on specific and non-specific immune defences in fish. J Exp Biol 201:165–168PubMedGoogle Scholar
  12. Leonardi MO, Klempau AE (2003) Artificial photoperiod influence on the immune system of juvenile rainbow trout (Oncorhynchus mykiss) in the Southern Hemisphere. Aquaculture 221:581–591CrossRefGoogle Scholar
  13. Markkula SE, Salo HM, Immonen AK, Jokinen EI (2005a) Effects of short- and long-term ultraviolet B irradiation on the immune system of the common carp (Cyprinus carpio). Photochem Photobiol 81:595–602CrossRefPubMedGoogle Scholar
  14. Markkula SE, Karvonen A, Salo HM, Valtonen ET, Jokinen EI (2005b) Ultraviolet B irradiation affects resistance of rainbow trout (Oncorhynchus mykiss) against bacterium Yersinia ruckeri and trematode Diplostomum spathaceum. Photochem Photobiol 83:1263–1269CrossRefGoogle Scholar
  15. Moser R (2000) Mysteriöses Bachforellensterben 1997 und 1999. Mitgliederzeitschrift des Sportanglerbundes Vöcklabruck 3:4Google Scholar
  16. Ouabius ES, Krupp G, Secombes CJ (2005) Polychlorinated biphenyl 126 affects expression of genes involved in stress-immune interaction in primary cultures of rainbow trout anterior kidney cells. Environ Toxicol Chem 24:3053–3060CrossRefGoogle Scholar
  17. Peters G, Schwarzer R (1985) Changes in hematopoietic tissue of rainbow trout under influence of stress. Dis Aquat Org 1:1–10CrossRefGoogle Scholar
  18. Press CM (1998) Immunology of fishes. In: Pastoret PP, Griebel P, Bazin H, Govaerts A (eds) Handbook of vertebrate immunology. Academic Press, London, pp 3–62Google Scholar
  19. Puangkaew J, Kiron V, Somamoto T, Okamoto N, Satoh S, Takeuchi T, Watanabe T (2004) Nonspecific immune response of rainbow trout (Oncorhynchus mykiss Walbaum) in relation to different status of vitamin E and highly unsaturated fatty acids. Fish Shellfish Immunol 16:25–39CrossRefPubMedGoogle Scholar
  20. Raida MK, Buchmann K (2008) Development of adaptive immunity in rainbow trout, Oncorhynchus mykiss (Walbaum) surviving an infection with Yersinia ruckeri. Fish Shellfish Immunol 25:533–541CrossRefPubMedGoogle Scholar
  21. Salo HM, Aaltonen TM, Markkula SE, Jokinen EI, Penttila HT (2000) Comparative effects of UVA and UVB irradiation on the immune system of fish. Photochem Photobiol 56:154–162CrossRefGoogle Scholar
  22. Scapigliati G, Scalia D, Marras A, Meloni S, Mazzini M (1999) Immunoglobulin levels in the teleost sea bass Dicentrarchus labrax (L.) in relation to age, season, and water oxygenation. Aquaculture 174:207–212CrossRefGoogle Scholar
  23. Schwaiger J, Gerst M, Römer C, Ferling H (2006) Studie zum Auftreten des Bachforellensterbens im Mühlbach, Kanton St. Gallen, Schweiz. Final report 2006Google Scholar
  24. Shigdar S, Cook D, Jones P, Harford A, Ward AC (2007) Blood cells of Murray cod Maccullochella peelii peelii (Mitchell). J Fish Biol 70:973–980CrossRefGoogle Scholar
  25. Siwicki AK, Anderson DP (1993) Nonspecific defense mechanisms assay in fish. II. Potential killing activity of neutrophils and macrophages, lysozyme activity in serum and organs and total immunoglobulin (T-Ig) levels in serum. Fish diseases diagnosis and prevention’s methods. FAO-Project GCP/INT/526/JPN, IFI Olsztyn: 105–112Google Scholar
  26. Tierney KB, Farell AP, Kennedy CJ (2004) The differential leucocyte landscape of four teleosts: juvenile Oncorhynchus kisutch, Clupea pallasi, Culaea inconstans and Pimephales promelas. J Fish Biol 65:906–919CrossRefGoogle Scholar
  27. Zuasti A, Ferrer C (1989) Haemopoiesis in the head kidney of Sparus aurata. Arch Histol Cytol 52:249–255CrossRefPubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2009

Authors and Affiliations

  • Franz Lahnsteiner
    • 1
    Email author
  • Reinhard Haunschmid
    • 2
  • Nabil Mansour
    • 1
  1. 1.Department of Organismic BiologyUniversity of SalzburgSalzburgAustria
  2. 2.Bundesamt für WasserwirtschaftInstitut für Gewässerökologie, Fischereibiologie und SeenkundeMondseeAustria

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