Cellular and Molecular Life Sciences CMLS

, Volume 60, Issue 3, pp 607–616

Dual effect of cannabinoid CB1 receptor stimulation on a vanilloid VR1 receptor-mediated response

  • H. Hermann
  • L. De Petrocellis
  • T. Bisogno
  • A. Schiano Moriello
  • B. Lutz
  • V. Di Marzo
Research Article

DOI: 10.1007/s000180300052

Cite this article as:
Hermann, H., De Petrocellis, L., Bisogno, T. et al. CMLS, Cell. Mol. Life Sci. (2003) 60: 607. doi:10.1007/s000180300052

Abstract.

Cannabinoid CB1 receptors and vanilloid VR1 receptors are co-localized to some extent in sensory neurons of the spinal cord and dorsal root ganglia. In this study, we over-expressed both receptor types in human embryonic kidney (HEK)-293 cells and investigated the effect of the CB1 agonist HU-210 on the VR1-mediated increase in intracellular Ca2+ ([Ca2+]i), a well-known response of the prototypical VR1 agonist capsaicin. After a 5-min pre-treatment, HU-210 (0.1 μM) significantly enhanced the effect of several concentrations of capsaicin on [Ca2+]i in HEK-293 cells over-expressing both rat CB1 and human VR1 (CB1-VR1-HEK cells), but not in cells over-expressing only human VR1 (VR1-HEK cells). This effect was blocked by the CB1 receptor antagonist SR141716A (0.5 μM), and by phosphoinositide-3-kinase and phospholipase C inhibitors. The endogenous agonist of CB1 and VR1 receptors, anandamide, was more efficacious in inducing a VR1-mediated stimulation of [Ca2+]i in CB1-VR1-HEK cells than in VR1-HEK cells, and part of its effect on the former cells was blocked by SR141716A (0.5 μM). Pre-treatment of CB1-VR1-HEK cells with forskolin, an adenylate cyclase activator, enhanced the capsaicin effect on [Ca2+]i. HU-210, which in the same cells inhibits forskolin-induced enhancement of cAMP levels, blocked the stimulatory effect of forskolin on capsaicin. Our data suggest that in cells co-expressing both CB1 and VR1 receptors, pre-treatment with CB1 agonists inhibits or stimulates VR1 gating by capsaicin depending on whether or not cAMP-mediated signalling has been concomitantly activated.

Key words. Anandamide; capsaicin; cannabinoid; vanilloid; receptor; signalling; pain. 

Copyright information

© Birkhäuser Verlag, 2003

Authors and Affiliations

  • H. Hermann
    • 1
  • L. De Petrocellis
    • 2
  • T. Bisogno
    • 2
  • A. Schiano Moriello
    • 2
  • B. Lutz
    • 1
  • V. Di Marzo
    • 2
  1. 1.Molecular Genetics of Behaviour, Max-Planck-Institute of Psychiatry, Kraepelinstr. 2-10, 80804 Munich (Germany) DE
  2. 2.Endocannabinoid Research Group, Institute of Biomolecular Chemistry (TB, VDM) and Institute of Cybernetics (LDP, ASM), Consiglio Nazionale delle Ricerche, Via Campi Flegrei 34, 80078 Pozzuoli, Naples (Italy), Fax + 39 081 8041770, e-mail: vdimarzo@icmib.na.cnr.itIT

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