Advertisement

Cellular and Molecular Life Sciences CMLS

, Volume 56, Issue 5–6, pp 543–547 | Cite as

The in vitro antiapoptotic effect of dehydroepiandrosterone sulfate in mouse thymocytes and its relation to caspase-3/caspase-6

  • C.-H. Yan
  • X.-F. Jiang
  • X. Pei
  • Y.-R. Dai

Abstract.

The effects of dehydroepiandrosterone sulfate (DHEAS) on thymocyte apoptosis induced by dexamethasone (DEX) were investigated. Apoptosis was measured by using agarose gel electrophoresis of DNA, the terminal deoxynucleotidyltransferase (TdT)- mediated dUTP nick end labeling (TUNEL) assay and flow cytometry. Our results showed that preincubation with 1×10−4 M DHEAS protected thymocytes from DEX-induced apoptosis in vitro. Moreover, we found no blocking effect on the DEX-induced activation of caspase-3 and caspase-6 by the preincubation of thymocytes with DHEAS. This may be interpreted to mean that the antagonism of DHEAS to DEX-induced apoptosis is not related to the activation of these downstream caspases which play a critical role in the execution of apoptosis.

Key words. Dehydroepiandrosterone sulfate; apoptosis; thymocytes; dexamethasone; caspase-3; caspase-6. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Birkhäuser Verlag Basel, 1999

Authors and Affiliations

  • C.-H. Yan
    • 1
  • X.-F. Jiang
    • 1
  • X. Pei
    • 1
  • Y.-R. Dai
    • 1
  1. 1.Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084 (China), Fax +86 010 6278 5505, e-mail: zhaizhh@tsinghua.edu.cnCN

Personalised recommendations