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Cellular and Molecular Life Sciences CMLS

, Volume 54, Issue 8, pp 833–850 | Cite as

Bacterial nonspecific acid phosphohydrolases: physiology, evolution and use as tools in microbial biotechnology

  • G. M. Rossolini
  • S. Schippa
  • M. L. Riccio
  • F. Berlutti
  • L. E. Macaskie
  • M. C. Thaller

Abstract.

Bacterial nonspecific acid phosphohydrolases (NSAPs) are secreted enzymes, produced as soluble periplasmic proteins or as membrane-bound lipoproteins, that are usually able to dephosphorylate a broad array of structurally unrelated substrates and exhibit optimal catalytic activity at acidic to neutral pH values. Bacterial NSAPs are monomeric or oligomeric proteins containing polypeptide components with an M r of 25 – 30 kDa. On the basis of amino acid sequence relatedness, three different molecular families of NSAPs can be distinguished, indicated as molecular class A, B and C, respectively. Members of each class share some common biophysical and functional features, but may also exhibit functional differences. NSAPs have been detected in several microbial taxa, and enzymes of different classes can be produced by the same bacterial species. Structural and phyletic relationships exist among the various bacterial NSAPs and some other bacterial and eucaryotic phosphohydrolases. Current knowledge on bacterial NSAPs is reviewed, together with analytical tools that may be useful for their characterization. An overview is also presented concerning the use of bacterial NSAPs in biotechnology.

Key words. Acid phosphohydrolases; bacteria; genetics; physiology; molecular evolution; microbial biotechnology. 

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Copyright information

© Birkhäuser Verlag Basel, 1998

Authors and Affiliations

  • G. M. Rossolini
    • 1
  • S. Schippa
    • 2
  • M. L. Riccio
    • 1
  • F. Berlutti
    • 2
  • L. E. Macaskie
    • 3
  • M. C. Thaller
    • 4
  1. 1.Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Via Laterina 8, I-53100 Siena (Italy), Fax +39 577 263325, e-mail: Rossolini@unisi.itIT
  2. 2.Istituto di Microbiologia, Università‘La Sapienza’, Rome (Italy)IT
  3. 3.School of Biological Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT (UK)GB
  4. 4.Dipartimento di Biologia, Cattedra di Microbiologia Applicata, Università di Roma ‘Tor Vergata’, Rome (Italy)IT

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