Stomatal guard cell responses to kinetin and natriuretic peptides are cGMP-dependent

  • 124 Accesses

  • 56 Citations


Immunological evidence suggests that plants contain natriuretic peptides (NPs) and furthermore (3- [125I]iodotyrosol28) rat atrial NP (rANP) binds specifically to plant membranes. rANP and immunoaffinity-purified plant NP analogues also promote concentration-dependent stomatal opening. Here we report that kinetin, a synthetic cytokinin, and rANP induce stomatal opening in Tradescantia albiflora and that the effect of rANP is critically dependent on the secondary structure of the peptide hormone. The native circular molecule is active, whereas the linearized molecule shows no biological activity. Furthermore, kinetin- and rANP-induced stomatal opening is reversibly inhibited by two in hibitors of guanylate cyclase, LY 83583 and methylene blue. Stomatal opening is also induced in a concentration-dependent manner by the cell-permeant cyclic guanosine-3′,5′-monophosphate (cGMP) analogue 8-Br-cGMP, and this effect is prevented by the stomatal closure promoting plant hormone abscisic acid (ABA). We conclude that in guard cells kinetin and rANP pathways operate via guanylate cyclase upregulation, and we propose that ABA-induced closure is not cGMP-dependent.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

Subscribe to journal

Immediate online access to all issues from 2019. Subscription will auto renew annually.

US$ 199

This is the net price. Taxes to be calculated in checkout.

Author information

Additional information

Received 1 October 1997; received after revision 2 December 1997; accepted 6 January 1998

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Pharmawati, M., Billington, T. & Gehring, C. Stomatal guard cell responses to kinetin and natriuretic peptides are cGMP-dependent. CMLS, Cell. Mol. Life Sci. 54, 272–276 (1998) doi:10.1007/s000180050149

Download citation

  • Key words. Natriuretic peptide; kinetin; abscisic acid; cGMP; stomata; guard cells.