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Cellular and Molecular Life Sciences

, Volume 75, Issue 15, pp 2719–2733 | Cite as

Engineering microbes for targeted strikes against human pathogens

  • In Young Hwang
  • Hui Ling Lee
  • James Guoxian Huang
  • Yvonne Yijuan Lim
  • Wen Shan Yew
  • Yung Seng Lee
  • Matthew Wook ChangEmail author
Review

Abstract

Lack of pathogen specificity in antimicrobial therapy causes non-discriminant microbial cell killing that disrupts the microflora present. As a result, potentially helpful microbial cells are killed along with the pathogen, altering the biodiversity and dynamic interactions within the population. Moreover, the unwarranted exposure of antibiotics to microbes increases the likelihood of developing resistance and perpetuates the emergence of multidrug resistance. Synthetic biology offers an alternative solution where specificity can be conferred to reduce the non-specific, non-targeted activity of currently available antibiotics, and instead provides targeted therapy against specific pathogens and minimising collateral damage to the host’s inherent microbiota. With a greater understanding of the microbiome and the available genetic engineering tools for microbial cells, it is possible to devise antimicrobial strategies for novel antimicrobial therapy that are able to precisely and selectively remove infectious pathogens. Herein, we review the strategies developed by unlocking some of the natural mechanisms used by the microbes and how these may be utilised in targeted antimicrobial therapy, with the promise of reducing the current global bane of multidrug antimicrobial resistance.

Keywords

Targeted therapy Synthetic biology Infectious pathogen Live biotherapeutics Microbiome Phage engineering Antimicrobial peptide Antibiotic resistance 

Notes

Acknowledgements

This work was supported by the Summit Research Program of the National University Health System (NUHSRO/2016/053/SRP/05), the Synthetic Biology Initiative of the National University of Singapore (DPRT/943/09/14), the U.S. Defense Threat Reduction Agency (HDTRA1-13-0037) and the Ministry of Defence of Singapore (MINDEF, RE2016-074). We thank Dr. Ping Han for her comments on the manuscript.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biochemistry, Yong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore
  2. 2.NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI)National University of SingaporeSingaporeSingapore
  3. 3.Department of Paediatrics, Yong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore
  4. 4.Khoo Teck Puat-National University Children’s Medical InstituteNational University Health SystemSingaporeSingapore

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