SLC52A3 expression is activated by NF-κB p65/Rel-B and serves as a prognostic biomarker in esophageal cancer
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The human riboflavin transporter-3 (encoded by SLC52A3) plays a prominent role in riboflavin absorption. Interestingly, abnormal expression patterns of SLC52A3 in multiple types of human cancers have been recently noted. However, the molecular mechanisms underlying its dysregulation remain unclear. In this study, we find that SLC52A3 has two transcript variants that differ in the transcriptional start site, and encode different proteins: SLC52A3a and SLC52A3b. Importantly, aberrant expressions of SLC52A3 are associated with stepwise development of esophageal squamous cell carcinoma (ESCC) as well as the survival rates of ESCC patients. Functionally, SLC52A3a, but not SLC52A3b, strongly promotes the proliferation and colony formation of ESCC cells. Furthermore, SLC52A3 5′-flanking regions contain NF-κB p65/Rel-B-binding sites, which are crucial for mediating SLC52A3 transcriptional activity in ESCC cells. Chromatin immunoprecipitation and electrophoretic mobility shift assay reveal that p65/Rel-B bind to 5′-flanking regions of SLC52A3. Accordingly, NF-κB signaling upregulates SLC52A3 transcription upon TNFα stimulation. Taken together, these results elucidate the mechanisms underlying SLC52A3 overexpression in ESCC. More importantly, our findings identify SLC52A3 as both a predictive and prognostic biomarker for this deadly cancer.
KeywordsSLC52A3 Riboflavin TNFα NF-κB Rel-B Esophageal cancer
Electrophoretic mobility shift assay
Esophageal squamous cell carcinoma
Rapid amplification of cDNA ends
Reverse transcriptase polymerase chain reaction
TNF receptor death domain
Transcriptional start site
Phosphate buffer saline
Esophageal squamous cell carcinoma (ESCC) is the sixth most lethal malignancy in China, with an incidence of 21.62/105 . Nutritional imbalance has been suggested as one of the risk factors for ESCC [2, 3, 4, 5]. Notably, a few reports indicated that lack of dietary riboflavin was associated with high risk for ESCC [6, 7]. However, the biological significance of riboflavin in the context of ESCC remains unknown. Many studies suggested that riboflavin played a role in immune system. Particularly, riboflavin deficiency affects cytokine production, and riboflavin-deprived cells released less IL-10 (anti-inflammatory) and more TNFα (pro-inflammatory) [8, 9, 10, 11].
Human riboflavin transporter-3 (encoded by SLC52A3) is a trans-membrane protein that has been shown to play an important role in the absorption of riboflavin and regulation of riboflavin tissue distribution [12, 13]. Humans and other mammals (which lack the ability to synthesize riboflavin endogenously) obtain riboflavin from exogenous sources including dietary and normal microflora of large intestine . SLC52A3 was reported as the most efficient transporter of riboflavin . Notably, different groups found that riboflavin deficiency increased the expression of SLC52A3 [13, 16, 17], implying a negative feedback regulatory mechanism maintaining riboflavin homeostasis.
SLC52A3 has been implicated in the biology of several tumor types, including those from stomach and cervix [17, 18, 19]. It was also reported that SLC52A3 is upregulated in ESCC and glioma, compared with nonmalignant adjacent tissue. Moreover, SLC52A3 enhanced the proliferation of ESCC and glioma cells [20, 21]. However, little is known concerning how the expression of SLC52A3 is regulated at the transcriptional level in ESCC cells. More importantly, the clinical significance and biological relevance of SLC52A3 expression remain to be elucidated.
Materials and methods
Cell cultures and treatment
Cell lines used in this study were previously described [22, 23]. ESCC cell lines (KYSE150, KYSE180, KYSE510, and TE3) were maintained in 1640 medium (Thermo Fisher Scientific) containing 10% fetal bovine serum (GIBCO). SHEEC and SHEE cells were cultured in Dulbecco’s modification of Eagle’s medium Dulbecco (DMEM)/F12 medium (Thermo Fisher Scientific) with 10% newborn bovine serum (ExcellBiology). Immortalized esophageal epithelial cell lines (NE2 and NE3) were cultured in a 1:1 mixture of defined keratinocyte serum-free medium (Thermo Fisher Scientific) and EpiLife (Thermo Fisher Scientific). HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum. All cells were incubated at 37 °C in a humidified atmosphere of 5% CO2 in air and maintained in media supplemented with penicillin-G (100 units/mL) and streptomycin (100 μg/mL).
In functional assays, KYSE150 and KYSE510 cells were seeded into 60 mm cell culture dishes at the density of 5 × 105 cells per dish and treated with various concentrations TNFα (0, 20, 100 or 200 ng/mL, an NF-κB inducer, Promega) for 0, 3, or 6 h, respectively. In drug treatment experiments, cells were pretreated with QNZ (500 nM, a NF-κB inhibitor, Selleck) or JSH-23 (300 nM, an NF-κB inhibitor, Selleck) for 24 h, followed by TNFα (20 ng/mL, ab NF-κB inhibitor, Promega) treatment for 6 h.
Sample collection and tissue microarray construction
For the retrospective survival analysis study, immunohistochemical staining was performed on ESCC tissue microarrays (TMA) constructed from paraffin-embedded specimens surgically resected at Shantou Central Hospital from 1987 to 1997 (246 cases) and 2007 to 2014 (290 cases). Tissue microarrays were constructed based on standard techniques as previously described . Information on gender, age, stage of disease, and histopathologic characteristics was obtained from the medical records. Patients’ data were summarized in Supplementary Table S1 and Table S2. Data analysis indicated that there were no survival advantages associated with the use of radiotherapy or chemotherapy compared with the surgery-alone group, so we assessed the patients’ survival together. Kaplan–Meier survival evaluation revealed that the prognosis of patients was significantly associated with the histological grade, regional lymph node metastasis, and pTNM stage (P < 0.05). The patients who suffered from severe post-operative complications and those who died of other tumors or other causes were excluded. The study was approved by the governmental ethics committee.
To find out the expression pattern of SLC52A3 during the progression from normal esophagus to ESCC, 39 paraffin-embedded specimens resected at Shantou Central Hospital were selected for immunohistochemical analysis in the study. The normal esophagus and some lesions of hyperplasia, high-grade dysplasia, including all noninvasive neoplastic epithelia, previously called carcinoma in situ, and ESCC were evaluated in each sample. The normal epithelia were seen in 20 samples, while hyperplasia was present in 13 cases, lesions of high-grade dysplasia in 21 sections, and ESCC tissues in 37 of the 39 samples.
SLC52A3a and SLC52A3b polyclonal antibody production
Rabbit anti-SLC52A3a and anti-SLC52A3b polyclonal antibody were produced by Zhoushan Bio-Technique Co., Ltd., Shandong, China. They used human SLC52A3a-unique sequence LRLFSSADFCNLHCPA (16 C-terminal amino acids) and SLC52A3b-unique sequence SIRPVGLLPLRTPHP (15 C-terminal amino acids) designed immunizing peptide and confirmed their stringent specificity using recombinant human SLC52A3 polypeptide by western blot.
Immunohistochemistry (IHC) was performed as described in our previous studies . Briefly, sections 4 μm thick were dewaxed in xylene, rehydrated in alcohol, and incubated in 3% hydrogen peroxide for 10 min to block endogenous peroxidase activity. Sections were incubated with 10% normal goat serum in PBS for 15 min at room temperature to block nonspecific binding. Then sections were incubated overnight at 4 °C with primary antibodies for SLC52A3 antibody (1:50, Abgent), SLC52A3a antibody (1:50, Zhoushan Bio-Technique), or SLC52A3b antibody (1:50, Zhoushan Bio-Technique). After rinsing with PBS, slides were incubated for 10 min at 37 °C with HRP Polymer Conjugate (ZYMED, USA). Subsequently, slides were stained with 0.003% 3,3-diaminobenzide tetrahydrochloride and 0.005% hydrogen peroxide in 0.05 M Tris–HCl (pH 7.2), and counterstained with hematoxylin, dehydrated, and mounted. Positive reactions were defined as those showing brown signals in the esophageal squamous cell cytoplasm, nucleus or membrane. Each separate tissue core was scored on the basis of the intensity and area of the positive staining. The intensity of positive staining was scored: 0, negative; 1, weak staining; 2, moderate staining; 3, strong staining. The percentage of positive cells was scored on a 0–4 scale: 0, 0–5%; 1, 6–25%; 2, 26–50%; 3, 51–75%; 4, > 75%. The total score was obtained by multiplying the two scores above producing a total score range of 0–12. For statistical analysis, we treated all the samples with a total score of 0–4 as low (−) and of 5–12 as high (+).
Mapping of the SLC52A3 gene transcriptional start sites (TSSs)
Total RNA from cell lines were extracted using TRIzol reagent (Invitrogen). Reverse transcription and rapid amplification of cDNA ends (RACE) was performed with the 5′-Full RACE Kit (TaKaRa, Dalian, China) in accordance with the manufacturer’s instructions. The final PCR product was extracted and purified from 2% agarose gel, cloned into pGEM-T Vector (Promega). Plasmid DNA from four different colonies was sequenced. Gene-specific primers used for 5′RACE experiments were listed in Supplementary Table S3.
Cloning and sequence analysis of full-length SLC52A3 cDNA
SLC52A3 sequences were cloned by reverse transcriptase polymerase chain reaction (RT-PCR). Briefly, RNA was extracted from ESCC cell lines (EC8712) with TRIzol reagent (Invitrogen) and reverse transcribed using a reverse transcription system (TaKaRa). SLC52A3a and SLC52A3b coding sequences were amplified using the cloning primers listed in Supplementary Table S3, after which they were ligated into the pEASY-Blunt Simple Cloning Vector (TRANSGEN BIOTECH) and verified by complete sequencing.
Construction of GFP-tagged SLC52A3a and SLC52A3b expression plasmids
The coding region of SLC52A3a and SLC52A3b was amplified and cloned into the HindIII and BamHI sites of pEGFP-C1 vector (Clontech) to generate the GFP-SLC52A3a and GFP-SLC52A3b expression vector.
Confocal immunofluorescence microscopy
The immunofluorescence staining was performed as described previously . In brief, GFP-tagged SLC52A3a and SLC52A3b constructs were transfected into KYSE150 cells. At 48 h post-transfection, the cells were fixed in 4% paraformaldehyde for 10 min, after which they were rinsed with PBS and permeabilized in 0.1% Triton X-100 for 10 min. Nonspecific binding was blocked by incubating the cells with 5% normal donkey serum (Jackson ImmunoResearch) in PBS for 60 min. Subsequently, cells were incubated with antibodies against GFP (Santa Cruz Biotechnology), and then, cells were probed with the Alexa Fluor 488-conjugated AffiniPure donkey–anti-mouse secondary antibody (Jackson ImmunoResearch) and counterstained with DAPI (Sigma-Aldrich). Cells were analyzed using a Zeiss LSM880 confocal microscope (Zeiss). For common cell immunofluorescence, cells were seeded on a coverslip and incubated for 24 h. Pass by fixation and blocking, cells were incubated with primary SLC52A3 polyclonal antibodies (Abgent) in PBS for 4 h at room temperature and were probed with donkey–anti-rabbit Dylight 594 secondary antibodies (Jackson ImmunoResearch) in PBS for 1 h. To visualize the cell nucleus, cells were incubated with DAPI (Sigma-Aldrich) for 15 min and were imaged using an FV-1000 confocal microscopy (Olympus).
shRNA lentivirus-mediated knockdown of SLC52A3
Recombinant lentivirus vectors pHBLV-U6-Luc-T2A-Puro harboring a short-hairpin RNA sequence targeting SLC52A3 (shSLC52A3-4#, shSLC52A3-6#, and control-shRNA) were produced by Hanbio Co., Ltd (Shanghai, China). To generate cells stably expressing shSLC52A3-4#, 6#, or the control-shRNA, lentivirus were used to infect KYSE180 and SHEEC cells, following the manufacturer’s instructions, when cells reached 40–50% confluence. Twenty-four hours later, cells were selected in medium containing 0.5 mg/mL puromycin (AMRESCO) for 20 days. After 4–5 passages in the presence of puromycin, the cultured cells were used for experiments.
Adenovirus-mediated overexpression of SLC52A3a and SLC52A3b
To generate cells overexpressing SLC52A3a and SLC52A3b, the SLC52A3a and SLC52A3b CDS sequences were ligated into the pHBAd-MCMV-Luc Vector (Hanbio Co., Ltd., Shanghai, China). Then, KYSE150 and KYSE180 cells were infected with adenoviral vectors overexpressing SLC52A3a (Ad-SLC52A3a), SLC52A3b (Ad-SLC52A3b), or control adenovirus (Ad-Luc), at the MOI (multiplicity of infection) of 100 after 24 h of seeding. Thirty-six hours later, cells were passaged or harvested for further analysis.
Cell proliferation assay
All cells were seeded in 96-well plates at 8000 cells per well. The CellTiter 96 aqueous nonradioactive cell proliferation assay was performed according to the manufacturer’s instructions (Promega) at 0, 24, 48, 72, and 96 h, respectively. 20 μL MTS was added to each well, and the plates were incubated for 2 h at 37 °C in a humidified, 5% CO2 atmosphere. MTS was bioreduced by the cells into a colored formazan product that displays absorbance at 490 nm. The absorbance was detected using a plate microplate reader (Multiskan MK3, Thermo). Raw data were normalized against those of the medium blank control.
Colony formation assay
Colony formation assay was performed as described previously . Briefly, transfected cells were plated at a density of 1000 cells per well in 6-well plates and incubated for 14 days at 37 °C with 5% CO2. After washing with 4 °C pre-cooled PBS, cultures were fixed with ice-cold methanol for 20 min and stained with hematoxylin for 15 min. Colonies were photographed and calculated using a FluorChem 8900 image analysis system (Alpha Innotech, Miami, FL, USA). Each experiment was performed in triplicate.
Measurement of riboflavin
Both intra- or extra-cellular concentrations of riboflavin were measured by high-performance liquid chromatography (HPLC) as described previously . Briefly, cells were plated at a density of 2 × 105 cells per well in 6-well plates, and culture medium was collected after 0, 24, 48, and 72 h, respectively, while cells were collected after 72 h. The concentrations of riboflavin in the collected culture medium were measured directly by HPLC. Cells were lysed by ultrasonic wave and riboflavin concentrations were measured by HPLC.
Reporter gene constructs
The human SLC52A3 5′-flanking region − 5076/− 2403 (translation initiation site for the SLC52A3 protein occurs at + 1) was generated by PCR using primers SLC52A3-5′FR-1F and SLC52A3-5′FR-1R (Supplementary Table S3). The amplified fragment from the genomic DNA of KYSE180 cells was digested with XhoI/BglII and inserted into the XhoI/BglII sites of pGL4.15[luc2P/Hygro] Vector (Promega), and the resulting plasmid was named pGL4 (− 5076/− 2403). Using the same methods, the luciferase reporter plasmids, pGL4 (− 3825/− 2403), pGL4 (− 3391/− 2403), pGL4 (− 2849/− 2403), pGL4 (− 3288/− 2403), pGL4 (− 3020/− 2403), pGL4 (− 2935/− 2403), pGL4 (− 2897/− 2403), pGL4 (− 2897/− 2495), pGL4 (− 2897/− 2583), pGL4 (− 2897/− 2672), pGL4 (− 2897/− 2743), and pGL4 (− 2897/− 2782) were generated. Different deletion fragments of SLC52A3-5′FR (− 3020/− 2672) and luciferase reporter plasmids, pGL4 (Δ− 2935/− 2897), pGL4 (Δ− 2897/− 2849), pGL4 (Δ− 2935/− 2849), and pGL4 (Δ− 2782/− 2743), were constructed by GENEWIZ (Suzhou, China).
Dual-luciferase reporter assay
Dual-luciferase reporter assay was performed as described previously [29, 30]. Briefly, KYSE150 or HEK293T Cells were seeded into 96-well plates at the density of 1 × 104 cells/well and cultured for 16–24 h until grown to 70–90% confluence. They were then co-transfected with a firefly luciferase expressing plasmid (1 μg), and a renilla luciferase-expressing plasmid (20 ng) (pRL-TK, Promega) as an internal control, using LIPOFECTAMINE 3000 Transfection Reagent (Thermo Fisher Scientific) in accordance with the manufacturer’s protocol. 48 h later, cells were harvested using passive lysis buffer (Promega). Luciferase activity was analyzed using the GloMax 96 Microplate Luminometer (Promega). Values for each group are expressed as the mean ± standard deviation (SD) for three separate experiments.
Western blot analysis
Whole cell protein extracts collected from cells were prepared in 1× Laemmli Sample Buffer (Bio-Rad), nuclear cell protein extracts were isolated by NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific), and membrane protein were isolated by Minute Plasma Membrane Protein Isolation Kit (Invent Biotechnologies, Inc.). The protein concentration was estimated by the Pierce 660 nm Protein Assay (Thermo). An equal amount of tissues lysates was electrophoresed on 10% polyacrylamide gel using standard methodology. Then, the lysates were transferred to PVDF membranes (Roche). The membranes were blocked in blocking buffer for 1 h followed by the addition of the primary antibody for 12 h at 4 °C. The membranes were then washed and incubated with a secondary antibody coupled to horseradish peroxidase for 1 h at room temperature. Antigen–antibody complexes were detected by Western blot luminol reagent (Santa Cruz Biotechnology). The primary antibodies used are as follows, anti-Ikkα, anti-Ikkβ, anti-phospho-Ikkα/β, anti-IκBα, anti-phospho-IκBα, anti-NF-κB p65 and anti-phospho-NF-κB p65 (ser536) (Cell Signaling Technology); anti-Rel-B, anti-NF-κB p50, anti-SLC52A3, anti-Integrin-α5, anti-Lamin A/C, and anti-β-actin (Santa Cruz Biotechnology); anti-GAPDH (Sigma). Image acquisition and quantitative analysis were carried out using the ChemiDoc XRS imaging system (Bio-Rad).
RNA extraction and quantitative real-time PCR
Total RNA was extracted from cells with TRIzol reagent (Invitrogen) in accordance with the manufacturer’s instructions. Reverse transcription (RT) and real-time PCR were performed as described earlier . Briefly, complementary DNA (cDNA) was generated from 1 μg total RNA in a final volume of 20 μL with Reverse Transcription System (TaKaRa). For the normalization of the amount of each transcript, the housekeeping gene ACTB (β-actin) was used as the internal control. The quantitative RT-PCR assay was carried out with the 7500 Real-Time PCR Systems system (Applied Biosystems) using SYBR Premix Ex Taq (TaKaRa) in accordance with the manufacturer’s instructions. Primers for quantitative RT-PCR are shown in Supplementary Table S3. The comparative delta–delta Ct (2−ΔΔCT) method was used to calculate relative expression levels .
Chromatin immunoprecipitation (ChIP) analysis was performed using EZ-Magna ChIP A/G Chromatin Immunoprecipitation kit (Millipore) in accordance with the manufacturer’s instructions. Briefly, KYSE150 cells were seeded into 100 mm cell culture dishes at the density of 1 × 105 cells per dish. Following formaldehyde crosslinking, cell lysate was sonicated on wet ice and then centrifuged to precipitate the debris. Input sample (5%) was collected before immunoprecipitation. Supernatant containing cross-linked chromatin was incubated overnight at 4 °C with 1 μg of specific antibodies, including anti-STAT3 (Santa Cruz), anti-NF-κB p65 (Santa Cruz), anti-Rel-B (Santa Cruz), anti-RNA polymerase II (Millipore), and normal mouse IgG (Millipore). After incubation, samples were subjected to DNA purification. Finally, purified DNA was analyzed by real-time PCR (qPCR) using SLC52A3 5′-flanking region primers (Supplementary Table S3), according to procedures described previously .
Electrophoretic mobility shift assay
LightShift Chemiluminescent Electrophoretic mobility shift assay (EMSA) kit and Chemiluminescent Nucleic Acid Detection Module were purchased from Thermo Fisher Scientific. Purified biotin-labeled probes were synthesized from Sangon Biotech, Shanghai, China. The probe sequences were in the Supplementary Table S3. Nuclear extracts of KYSE150 cells were isolated by NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific). For binding assays, 2 μg nuclear extracts were incubated with 20 fmol probes at room temperature for 25 min in 20 μL reaction buffers. Electrophoreses, transferring to a nylon membrane and crosslinking at high temperature (120 °C), were performed in accordance with the manufacturer’s protocol. To confirm the specificity of the binding between transcription factors and the probes, antibodies anti-STAT3, anti-NF-κB p65, anti-Rel-B (Santa Cruz), and 2 μg nuclear extracts were incubated for 10 min at 4 °C.
Data analysis was performed using SPSS 16.0 software. A two-tailed independent sample t test was used to determine the significance of differences between groups. Differences were considered statistically significant at P < 0.05 (*), P < 0.01 (**), and P < 0.01 (***). Data are plotted as mean ± SD.
SLC52A3 protein was upregulated during the stepwise development of ESCC
To further explore the clinical significance of the expression of SLC52A3 protein, we next performed IHC in a large independent cohort with 246 ESCC patients. Importantly, survival analysis revealed that SLC52A3 protein expression was a prognostic factor in ESCC. Specifically, stronger immunoreactivity of SLC52A3 in the membrane and cytoplasm was associated with a better survival (P = 0.028, Fig. 1c). In contrast, higher expression of SLC52A3 in nucleus was significantly associated with poor prognosis (P = 0.017, Fig. 1c).
Identification of a novel SLC52A3 isoform in ESCC
This new SLC52A3b variant encodes a protein of 415 amino acids with predicted molecular mass of 45 kDa. We next constructed GFP-tagged expression plasmids for SLC52A3a and SLC52A3b (Fig. 2d). Confocal immunofluorescence microscopy showed that the majority of SLC52A3a expressed in cell membrane and nucleus, while SLC52A3b localized in cell cytoplasm (Fig. 2e). We next measured endogenous SLC52A3 proteins in ESCC cell lines and primary tissues by Western blotting, and again, both isoforms were evident in ESCC cell lines (Fig. 2f). Notably, higher protein levels of SLC52A3a and SLC52A3b were also observed in ESCC tumor samples compared with matched normal tissues, in agreement with our earlier IHC results (Fig. 2f).
High SLC52A3a nucleic expression correlates with poor prognosis in ESCC patients
Functional roles of SLC52A3 in ESCC
We next sought to investigate the biological functions of SLC52A3 using ESCC cell line models. First, we determined the transport capacity of riboflavin by either SLC52A3a or SLC52A3b in KYSE150 and KYSE510 cells by measuring both riboflavin consumption in cell culture medium and intracellular riboflavin concentration using high-performance liquid chromatography (HPLC). Importantly, our results showed that cells expressing SLC52A3a exhibited faster riboflavin consumption and maintained higher intracellular concentration of riboflavin compared to control cells. In contrast, expression of SLC52A3b did not cause any alterations in either riboflavin consumption or intracellular riboflavin concentration (Supplementary Figure S3), suggesting that SLC52A3a has higher capacity in transporting riboflavin than SLC52A3b. Importantly, shRNA-mediated knockdown of SLC52A3 (shSLC52A3-6#) markedly decreased the proliferation of both KYSE180 and SHEEC cells (Fig. 4c). ESCC colony formation was also potently inhibited upon silencing of SLC52A3 (Fig. 4d). We next ectopically expressed either isoforms, and noted that overexpression of SLC52A3a significantly increased the proliferation of both KYSE150 and KYSE180 cells. In contrast, overexpression of SLC52A3b did not produce the same effect. These data together suggest that isoform SLC52A3a, but not SLC52A3b, promotes the malignant phenotype of ESCC cells (Fig. 4e).
Identification of transcriptional regulatory elements in SLC52A3 5′-flanking regions
To further investigate the transcriptional regulatory elements of SLC52A3, a series of 5′-flanking regions spanning − 3020/− 2403 were studies by progressive 3′-deletions, which initially increased the reporter activity and then followed by decreases. Specifically, deletion from nt − 2403 to nt − 2495 caused a 16-fold increase in luciferase activity, whereas deletion from − 2583/− 2672, − 2672/− 2743, and − 2743/− 2782 markedly decreased the activity (Fig. 5c, d). This result indicated that 5′-flanking regions of SLC52A3 gene contained at least five transcriptional regulatory elements, namely TBS1–TBS5, as illustrated in Fig. 5e.
Further progressive deletions of either TBS1, TBS2, or both TBS1/TBS2 did not alter the reporter activity. However, the deletion of TBS3 sharply and significantly reduced reporter activity (Fig. 5f), suggesting that a key cis-acting element exists in this segment (− 2782/− 2743) which regulates SLC52A3 transcription in ESCC cells.
NF-κB p65/Rel-B factor binds to the NF-κB-binding site within TBS3 and activates SLC52A3 transcription
NF-κB family members modulate the transcription of a number of genes that regulate inflammation, apoptosis, and tumorigenesis [33, 34, 35, 36]. Tumor necrosis factor α (TNFα) is among the best-characterized activators upstream of NF-κB signaling pathway . To explore whether NF-κB p65/Rel-B regulate the transcription of SLC52A3, KYSE150, and KYSE510 cell lines were treated with various concentrations of TNFα. As expected, TNFα stimulation increased NF-κB activity, as evident by the enhanced phosphorylation of IKKα/β, as well as increased expression of Rel-B and p50. Notably, activation of NF-κB pathway resulted in strong upregulation of SLC52A3 expression, particularly SLC52A3a (Fig. 6d). On the other hand, treatment of NF-κB inhibitors QNZ or JSH-23 substantially decreased the expression levels of SLC52A3a and SLC52A3b (Fig. 6e). NF-κB signaling pathway-related proteins and SLC52A3, specially SLC52A3a, were also decreased in KYSE150 cells (Fig. 6f).
To further confirm the transcriptional regulation of NF-κB on TBS3 segment, we performed progressive deletions for reporter luciferase assay under the treatment of TNFα. Importantly, TNFα stimulated the reporter activity of all constructs except for the one without NF-κB-binding sequences (Fig. 6g, upper panel). Similar results were obtained by serial 3′ deletions (Fig. 6g, lower panel). These results together suggest that NF-κB signaling activates SLC52A3 transcription through direct binding to its canonical sequences within SLC52A3 5′-flanking region.
Recent results demonstrated profound epigenomic dysregulation of ESCC transcriptomes [38, 39, 40, 41]. In the present study, we found that SLC52A3 was transcriptionally upregulated in both esophagus dysplasia and ESCC, and high nucleus expression of SLC52A3 was correlated with poor prognosis. Importantly, SLC52A3 promoted cell proliferation and colony formation in ESCC. Our results further showed that SLC52A3 had two transcript variants that differ in their transcriptional start sites (TSS) and encoded two different proteins. The previous studies reported that intron retention may be ascribed to the dysfunction of splicing factors or weaker splice sites [42, 43]. However, whether alternative transcription start site and splicing are functionally linked remains unclear. Further investigations are thus warranted in the future.
A previous study reported the minimal promoter of SLC52A3 , which were in agreement with our data. We here, however, identified an additional TSS of SLC52A3 using RACE. Luciferase reporter assay revealed that 5′-flanking regions of SLC52A3 contain at least five transcriptional regulatory elements (TBS1–TBS5). Furthermore, our results revealed and validated a functional-binding site for NF-κB p65/Rel-B within TBS3. Interestingly, the previous GWAS results showed that an SNP locus rs13042395 (C>T), located − 8093 nt upstream of SLC52A3 TSS, was associated with increased ESCC risk [45, 46]. A study demonstrated that the CC genotype of this SNP conferred stronger transcription activity for SLC52A3 . Together with our results, these findings suggest a complex regulatory mechanism controlling SLC52A3 transcription in ESCC.
In conclusion, our findings establish the clinical significance of SLC52A3 expression in the pathogenesis of ESCC, and highlight both the predictive and prognostic values of this pro-growth protein. We further identified isoform-specific functions of SLC52A3, in which SLC52A3a, but not SLC52A3b, promotes the malignant phenotypes of ESCC cells. Mechanistically, we reveal a direct transcription regulation of NF-κB p65/Rel-B on the expression of SLC52A3, providing important insights into the tumorigenesis and progression of ESCC.
This work was supported by a grant from the Natural Science Foundation of China-Guangdong Joint Fund (No. U1301227 and No. U1601229), the National Cohort of Esophageal Cancer of China (No. 2016YFC0901400), the National Natural Science Foundation of China (No. 81772532 and No. 81472613), and the Department of Education, Guangdong Government under the Top-tier University Development Scheme for Research and Control of Infectious Diseases. Lin DC was supported by the National Natural Science Foundation of China (81672786). We are grateful for assistance from the Central Laboratory at Shantou University Medical College, including Wen-Hong Luo and Hong-Jun Luo, for obtaining the riboflavin concentrations data by HPLC.
LL: data collection, data analysis, interpretation, and drafting; LYX, EML, DCL, and LDW: study design, study supervision, and final approval of the manuscript; XXP, XEX, and JZH: Tissue microarrays construction and immunohistochemical staining; JYW and ZYW: sample collection and follow-up; FL and JSZ: dual-luciferase reporter assay; WW: DNA cloning and confocal microscope; LDL: cell culture.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest with the contents of this article.
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