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Cellular and Molecular Life Sciences

, Volume 75, Issue 2, pp 335–354 | Cite as

Pathogenic mutation in the ALS/FTD gene, CCNF, causes elevated Lys48-linked ubiquitylation and defective autophagy

  • Albert LeeEmail author
  • Stephanie L. Rayner
  • Serene S. L. Gwee
  • Alana De Luca
  • Hamideh Shahheydari
  • Vinod Sundaramoorthy
  • Audrey Ragagnin
  • Marco Morsch
  • Rowan Radford
  • Jasmin Galper
  • Sarah Freckleton
  • Bingyang Shi
  • Adam K. Walker
  • Emily K. Don
  • Nicholas J. Cole
  • Shu Yang
  • Kelly L. Williams
  • Justin J. Yerbury
  • Ian P. Blair
  • Julie D. Atkin
  • Mark P. Molloy
  • Roger S. Chung
Original Article

Abstract

Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are fatal neurodegenerative disorders that have common molecular and pathogenic characteristics, such as aberrant accumulation and ubiquitylation of TDP-43; however, the mechanisms that drive this process remain poorly understood. We have recently identified CCNF mutations in familial and sporadic ALS and FTD patients. CCNF encodes cyclin F, a component of an E3 ubiquitin–protein ligase (SCFcyclin F) complex that is responsible for ubiquitylating proteins for degradation by the ubiquitin–proteasome system. In this study, we examined the ALS/FTD-causing p.Ser621Gly (p.S621G) mutation in cyclin F and its effect upon downstream Lys48-specific ubiquitylation in transfected Neuro-2A and SH-SY5Y cells. Expression of mutant cyclin FS621G caused increased Lys48-specific ubiquitylation of proteins in neuronal cells compared to cyclin FWT. Proteomic analysis of immunoprecipitated Lys48-ubiquitylated proteins from mutant cyclin FS621G-expressing cells identified proteins that clustered within the autophagy pathway, including sequestosome-1 (p62/SQSTM1), heat shock proteins, and chaperonin complex components. Examination of autophagy markers p62, LC3, and lysosome-associated membrane protein 2 (Lamp2) in cells expressing mutant cyclin FS621G revealed defects in the autophagy pathway specifically resulting in impairment in autophagosomal–lysosome fusion. This finding highlights a potential mechanism by which cyclin F interacts with p62, the receptor responsible for transporting ubiquitylated substrates for autophagic degradation. These findings demonstrate that ALS/FTD-causing mutant cyclin FS621G disrupts Lys48-specific ubiquitylation, leading to accumulation of substrates and defects in the autophagic machinery. This study also demonstrates that a single missense mutation in cyclin F causes hyper-ubiquitylation of proteins that can indirectly impair the autophagy degradation pathway, which is implicated in ALS pathogenesis.

Keywords

Ubiquitylation Phosphorylation CCNF Cyclin F Amyotrophic lateral sclerosis Frontotemporal dementia Motor neuron disease 

Notes

Acknowledgements

This research was supported by funding from the Motor Neurone Disease Research Institute of Australia (MNDRIA) (GIA1510, GIA1628, and GIA1715); MND Australia and National Health and Medical Research Council of Australia (NHMRC) Grants: APP1107644, APP1030513 and APP1095215, and NHMRC early career fellowship APP1036835. This research was supported by access to the Australian Proteomics Analysis Facility (APAF) established under the Australian Government’s NCRIS program. The authors acknowledge the support of the Macquarie University Centre for Motor Neuron Disease Research.

Supplementary material

18_2017_2632_MOESM1_ESM.xlsx (396 kb)
Supplementary material 1 (XLSX 396 kb)
18_2017_2632_MOESM2_ESM.pptx (7.7 mb)
Supplementary material 2 (PPTX 7857 kb)

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Albert Lee
    • 1
    • 2
    Email author
  • Stephanie L. Rayner
    • 1
    • 3
  • Serene S. L. Gwee
    • 1
  • Alana De Luca
    • 1
  • Hamideh Shahheydari
    • 1
  • Vinod Sundaramoorthy
    • 1
  • Audrey Ragagnin
    • 1
  • Marco Morsch
    • 1
  • Rowan Radford
    • 1
  • Jasmin Galper
    • 1
  • Sarah Freckleton
    • 1
  • Bingyang Shi
    • 1
  • Adam K. Walker
    • 1
  • Emily K. Don
    • 1
  • Nicholas J. Cole
    • 1
  • Shu Yang
    • 1
  • Kelly L. Williams
    • 1
  • Justin J. Yerbury
    • 4
  • Ian P. Blair
    • 1
  • Julie D. Atkin
    • 1
    • 5
  • Mark P. Molloy
    • 2
    • 3
  • Roger S. Chung
    • 1
  1. 1.Centre for Motor Neuron Disease Research, Department of Biomedical Sciences, Faculty of Medicine and Health SciencesMacquarie UniversityNorth RydeAustralia
  2. 2.Australian Proteome Analysis FacilityMacquarie UniversityNorth RydeAustralia
  3. 3.Department of Chemistry and Biomolecular Sciences, Faculty of Science and EngineeringMacquarie UniversityNorth RydeAustralia
  4. 4.Illawarra Health and Medical Research Institute, School of Biological SciencesUniversity of WollongongWollongongAustralia
  5. 5.Department of Biochemistry and GeneticsLa Trobe Institute for Molecular ScienceMelbourneAustralia

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