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Cellular and Molecular Life Sciences

, Volume 72, Issue 9, pp 1795–1809 | Cite as

Pax6 controls centriole maturation in cortical progenitors through Odf2

  • Marco A. Tylkowski
  • Kefei Yang
  • Sigrid Hoyer-Fender
  • Anastassia StoykovaEmail author
Research Article

Abstract

Cortical glutamatergic neurons are generated by radial glial cells (RGCs), specified by the expression of transcription factor (TF) Pax6, in the germinative zones of the dorsal telencephalon. Here, we demonstrate that Pax6 regulates the structural assembly of the interphase centrosomes. In the cortex of the Pax6-deficient Small eye (Sey/Sey) mutant, we find a defect of the appendages of the mother centrioles, indicating incomplete centrosome maturation. Consequently, RGCs fail to generate primary cilia, and instead of staying in the germinative zone for renewal, RGCs detach from the ventricular surface thus affecting the interkinetic nuclear migration and they exit prematurely from mitosis. Mechanistically, we show that TF Pax6 directly regulates the activity of the Odf2 gene encoding for the appendage-specific protein Odf2 with a role for the assembly of mother centriole. Our findings demonstrate a molecular mechanism that explains important characteristics of the centrosome disassembly and malfunctioning in developing cortex lacking Pax6.

Keywords

Centriole structure Transcriptional control 

Notes

Acknowledgments

The authors thank Prof. Dr. med Nicolai Miosge for technical and knowledge support regarding electron microscopy. Thanks are also due to Silke Schlott and Martina Daniel for excellent technical assistance. We thank T. Schweizer for proofreading. Marco A. Tylkowski was funded through Cluster of Excellence and DFG Research Center Nanoscale Microscopy and Molecular Physiology of the Brain.

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Copyright information

© Springer Basel 2014

Authors and Affiliations

  • Marco A. Tylkowski
    • 1
    • 2
  • Kefei Yang
    • 3
  • Sigrid Hoyer-Fender
    • 3
  • Anastassia Stoykova
    • 1
    • 2
    Email author
  1. 1.Research Group of Molecular Developmental Neurobiology, Department Molecular Cell BiologyMax-Planck Institute for Biophysical ChemistryGöttingenGermany
  2. 2.Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)GöttingenGermany
  3. 3.Johann-Friedrich-Blumenbach-Institute of Zoology and Anthropology, Developmental Biology, GZMB, Ernst-Caspari-HausGeorg-August-Universität GöttingenGöttingenGermany

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