Advertisement

Cellular and Molecular Life Sciences

, Volume 71, Issue 17, pp 3393–3408 | Cite as

Chromatin composition alterations and the critical role of MeCP2 for epigenetic silencing of progesterone receptor-B gene in endometrial cancers

  • Yongli Chu
  • Yanlin Wang
  • Guanghua Zhang
  • Haibin Chen
  • Sean C. Dowdy
  • Yuning Xiong
  • Fengming Liu
  • Run Zhang
  • Jinping LiEmail author
  • Shi-Wen JiangEmail author
Research Article

Abstract

Objective

To understand the epigenetic mechanism underlying the PR-B gene silencing in endometrial cancer (EC) cells, we compared the chromatin composition between transcriptionally active and silenced PR-B genes in EC cell lines and cancer tissues.

Methods

Chromatin Immunoprecipitation (ChIP) assay was performed to measure MBD occupancy and histone acetylation/methylation in transcriptionally active and silenced PR-B genes. PR-B-positive/-negative, as well as epigenetic inhibitor-treated/-untreated EC cells were used as study models. Real-time polymerase chain reaction (PCR) and Western blot analysis were applied to measure the mRNA and protein levels of PR-B, MBD, and histones.

Results

A close association among PR-B methylation, MBD binding and PR-B gene silencing was observed. Treatment with epigenetic inhibitors led to dynamic changes in the PR-B chromatin composition and gene expression. Increased H3/H4 acetylation and H3-K4 methylation, and decreased H3-K9 methylation were found to be associated with re-activation of silenced PR-B genes. MeCP2 knockdown resulted in a decreased MeCP2 binding to PR-B genes and an increased PR-B expression. ChIP analysis of MeCP2 binding to PR-B genes in the PR-B-positive/-negative EC samples confirmed the significant role of MeCP2 in PR-B silencing.

Conclusion

PR-B gene expression is regulated by a concerted action of epigenetic factors including DNA methylation, MBD binding, and histone modifications. MeCP2 occupancy of PR-B genes plays a critical role in PR-B gene silencing. These findings enriched our knowledge of the epigenetic regulation of PR-B expression in EC, and suggested that the epigenetic re-activation of PR-B could be explored as a potential strategy to sensitize the PR-B-negative endometrial cancers to progestational therapy.

Keywords

Progesterone receptor-B Epigenetic silencing Endometrial cancer DNA methylation Chromatin 

Notes

Acknowledgments

The authors would like thank Mrs. Lynn Caflisch for her strong secretarial support and Mrs. Ying Zhao for her superb technical assistance. Shi-Wen Jiang is supported by the Distinguished Cancer Scholarship of the Georgia Research Alliance (GRA). This work was partially funded by research grants from the National Institute of Health (NIH) (R01 HD 41577, Shi-Wen Jiang); the NIH/National Cancer Institute (NCI)-MD Anderson Uterine Cancer SPORE (Jinping Li, Shi-Wen Jiang); the NIH K12 training program (Sean Dowdy, Shi-Wen Jiang); a research grant from Merck Pharmaceiticals (Sean Dowdy, Shi-Wen Jiang); the research supplement from the Department of Obstetrics and Gynecology, Mayo Clinic and Mayo Medical School (Shi-Wen Jiang); the National Natural Science Foundation of China 81200420 and the Yantai Science Development Fund 2011219 (Yongli Chu); the Shangdong Natural Science Foundation ZR2012HL03 and 2011YD21014 (Yanlin Wang); the research start-up from Mercer University School of Medicine (Jinping Li); and seed grants from Mercer University (Jinping Li, Shi-Wen Jiang).

Conflict of interest

The authors declare that there are no conflicts of interest.

References

  1. 1.
    Sitruk-Ware R, Plu-Bureau G (1999) Progestins and cancer. Gynecol Endocrinol 13(Suppl 4):3–9PubMedCrossRefGoogle Scholar
  2. 2.
    Markman M (2005) Hormonal therapy of endometrial cancer. Eur J Cancer 41:673–675PubMedCrossRefGoogle Scholar
  3. 3.
    Pike MC, Pearce CL, Wu AH (2004) Prevention of cancers of the breast, endometrium and ovary. Oncogene 23:6379–6391PubMedCrossRefGoogle Scholar
  4. 4.
    Podratz KC, O’Brien PC, Malkasian GD Jr, Decker DG, Jefferies JA, Edmonson JH (1985) Effects of progestational agents in treatment of endometrial carcinoma. Obstet Gynecol 66:106–110PubMedGoogle Scholar
  5. 5.
    Figueroa-Casas PR, Ettinger B, Delgado E, Javkin A, Vieder C (2001) Reversal by medical treatment of endometrial hyperplasia caused by estrogen replacement therapy. Menopause 8:420–423PubMedCrossRefGoogle Scholar
  6. 6.
    Ramirez PT, Frumovitz M, Bodurka DC, Sun CC, Levenback C (2004) Hormonal therapy for the management of grade 1 endometrial adenocarcinoma: a literature review. Gynecol Oncol 95:133–138PubMedCrossRefGoogle Scholar
  7. 7.
    Lentz SS, Brady MF, Major FJ, Reid GC, Soper JT (1996) High-dose megestrol acetate in advanced or recurrent endometrial carcinoma: a Gynecologic Oncology Group Study. J Clin Oncol 14:357–361PubMedGoogle Scholar
  8. 8.
    Podczaski E, Mortel R (2001) Hormonal treatment of endometrial cancer: past, present and future. Best Pract Res Clin Obstet Gynaecol 15:469–489PubMedCrossRefGoogle Scholar
  9. 9.
    van Rijswijk RE, Vermorken JB (2000) Drug therapy for gynaecological cancer in older women. Drugs Aging 17:13–32PubMedCrossRefGoogle Scholar
  10. 10.
    Whitney CW, Brunetto VL, Zaino RJ, Lentz SS, Sorosky J, Armstrong DK, Lee RB (2004) Phase II study of medroxyprogesterone acetate plus tamoxifen in advanced endometrial carcinoma: a Gynecologic Oncology Group study. Gynecol Oncol 92:4–9PubMedCrossRefGoogle Scholar
  11. 11.
    Fiorica JV, Brunetto VL, Hanjani P, Lentz SS, Mannel R, Andersen W (2004) Phase II trial of alternating courses of megestrol acetate and tamoxifen in advanced endometrial carcinoma: a Gynecologic Oncology Group study. Gynecol Oncol 92:10–14PubMedCrossRefGoogle Scholar
  12. 12.
    Burnett AF, Bahador A, Amezcua C, Anastrozole (2004) An aromatase inhibitor, and medroxyprogesterone acetate therapy in premenopausal obese women with endometrial cancer: a report of two cases successfully treated without hysterectomy. Gynecol Oncol 94:832–834PubMedCrossRefGoogle Scholar
  13. 13.
    Williams SP, Sigler PB (1998) Atomic structure of progesterone complexed with its receptor. Nature 393:392–396PubMedCrossRefGoogle Scholar
  14. 14.
    Wagner BL, Norris JD, Knotts TA, Weigel NL, McDonnell DP (1998) The nuclear corepressors NCoR and SMRT are key regulators of both ligand- and 8-bromo-cyclic AMP-dependent transcriptional activity of the human progesterone receptor. Mol Cell Biol 18:1369–1378PubMedCentralPubMedGoogle Scholar
  15. 15.
    Charnock-Jones DS, Macpherson AM, Archer DF, Leslie S, Makkink WK, Sharkey AM, Smith SK (2000) The effect of progestins on vascular endothelial growth factor, oestrogen receptor and progesterone receptor immunoreactivity and endothelial cell density in human endometrium. Hum Reprod 15(Suppl 3):85–95PubMedCrossRefGoogle Scholar
  16. 16.
    Shiozawa T, Horiuchi A, Kato K, Obinata M, Konishi I, Fujii S, Nikaido T (2001) Up-regulation of p27Kip1 by progestins is involved in the growth suppression of the normal and malignant human endometrial glandular cells. Endocrinology 142:4182–4188PubMedCrossRefGoogle Scholar
  17. 17.
    Kester HA, Sonneveld E, van der Saag PT, van der Burg B (2003) Prolonged progestin treatment induces the promoter of CDK inhibitor p21 Cip1, Waf1 through activation of p53 in human breast and endometrial tumor cells. Exp Cell Res 284:264–273PubMedCrossRefGoogle Scholar
  18. 18.
    Amezcua CA, Lu JJ, Felix JC, Stanczyk FZ, Zheng W (2000) Apoptosis may be an early event of progestin therapy for endometrial hyperplasia. Gynecol Oncol 79:169–176PubMedCrossRefGoogle Scholar
  19. 19.
    Quinn MA, Cauchi M, Fortune D (1985) Endometrial carcinoma: steroid receptors and response to medroxyprogesterone acetate. Gynecol Oncol 21:314–319PubMedCrossRefGoogle Scholar
  20. 20.
    Kleine W, Maier T, Geyer H, Pfleiderer A (1990) Estrogen and progesterone receptors in endometrial cancer and their prognostic relevance. Gynecol Oncol 38:59–65PubMedCrossRefGoogle Scholar
  21. 21.
    Podratz KC (1990) Hormonal therapy in endometrial carcinoma. Recent Results Cancer Res 118:242–251PubMedCrossRefGoogle Scholar
  22. 22.
    Dai D, Wolf DM, Litman ES, White MJ, Leslie KK (2002) Progesterone inhibits human endometrial cancer cell growth and invasiveness: down-regulation of cellular adhesion molecules through progesterone B receptors. Cancer Res 62:881–886PubMedGoogle Scholar
  23. 23.
    Kumar NS, Richer J, Owen G, Litman E, Horwitz KB, Leslie KK (1998) Selective down-regulation of progesterone receptor isoform B in poorly differentiated human endometrial cancer cells: implications for unopposed estrogen action. Cancer Res 58:1860–1865PubMedGoogle Scholar
  24. 24.
    Sakaguchi H, Fujimoto J, Hong BL, Nakagawa Y, Tamaya T (2004) Drastic decrease of progesterone receptor form B but not A mRNA reflects poor patient prognosis in endometrial cancers. Gynecol Oncol 93:394–399PubMedCrossRefGoogle Scholar
  25. 25.
    Hanekamp EE, Kuhne EC, Smid-Koopman E, de Ruiter PE, Chadha-Ajwani S, Brinkmann AO, Burger CW, Grootegoed JA, Huikeshoven FJ, Blok LJ (2002) Loss of progesterone receptor may lead to an invasive phenotype in human endometrial cancer. Eur J Cancer 38(Suppl 6):S71–S72PubMedCrossRefGoogle Scholar
  26. 26.
    Pijnenborg JM, Romano A, Dam-de Veen GC, Dunselman GA, Fischer DC, Groothuis PG, Kieback DG (2005) Aberrations in the progesterone receptor gene and the risk of recurrent endometrial carcinoma. J Pathol 205:597–605PubMedCrossRefGoogle Scholar
  27. 27.
    Sasaki M, Dharia A, Oh BR, Tanaka Y, Fujimoto S, Dahiya R (2001) Progesterone receptor B gene inactivation and CpG hypermethylation in human uterine endometrial cancer. Cancer Res 61:97–102PubMedGoogle Scholar
  28. 28.
    Jin F, Dowdy SC, Xiong Y, Eberhardt NL, Podratz KC, Jiang SW (2005) Up-regulation of DNA methyltransferase 3B expression in endometrial cancers. Gynecol Oncol 96:531–538PubMedCrossRefGoogle Scholar
  29. 29.
    Xiong Y, Dowdy SC, Xue A, Shujuan J, Eberhardt NL, Podratz KC, Jiang SW (2005) Opposite alterations of DNA methyltransferase gene expression in endometrioid and serous endometrial cancers. Gynecol Oncol 96:601–609PubMedCrossRefGoogle Scholar
  30. 30.
    Baylin SB, Esteller M, Rountree MR, Bachman KE, Schuebel K, Herman JG (2001) Aberrant patterns of DNA methylation, chromatin formation and gene expression in cancer. Hum Mol Genet 10:687–692PubMedCrossRefGoogle Scholar
  31. 31.
    Esteller M, Herman JG (2002) Cancer as an epigenetic disease: DNA methylation and chromatin alterations in human tumours. J Pathol 196:1–7PubMedCrossRefGoogle Scholar
  32. 32.
    Jones PA, Baylin SB (2002) The fundamental role of epigenetic events in cancer. Nat Rev Genet 3:415–428PubMedCrossRefGoogle Scholar
  33. 33.
    Bird AP, Wolffe AP (1999) Methylation-induced repression—belts, braces, and chromatin. Cell 99:451–454PubMedCrossRefGoogle Scholar
  34. 34.
    Irvine RA, Lin IG, Hsieh CL (2002) DNA methylation has a local effect on transcription and histone acetylation. Mol Cell Biol 22:6689–6696PubMedCentralPubMedCrossRefGoogle Scholar
  35. 35.
    Shi Y (2007) Histone lysine demethylases: emerging roles in development, physiology and disease. Nat Rev Genet 8:829–833PubMedCrossRefGoogle Scholar
  36. 36.
    Dhasarathy A, Wade PA (2008) The MBD protein family-reading an epigenetic mark? Mutat Res 647:39–43PubMedCentralPubMedCrossRefGoogle Scholar
  37. 37.
    Tsukada Y, Ishitani T, Nakayama KI (2010) KDM7 is a dual demethylase for histone H3 Lys 9 and Lys 27 and functions in brain development. Genes Dev 24:432–437Google Scholar
  38. 38.
    Nishida M, Kasahara K, Oki A, Satoh T, Arai Y, Kubo T (1996) Establishment of eighteen clones of Ishikawa cells. Hum Cell 9:109–116PubMedGoogle Scholar
  39. 39.
    Xiong Y, Dowdy SC, Podratz KC, Jin F, Attewell JR, Eberhardt NL, Jiang SW (2005) Histone deacetylase inhibitors decrease DNA methyltransferase-3B messenger RNA stability and down-regulate de novo DNA methyltransferase activity in human endometrial cells. Cancer Res 65:2684–2689PubMedCrossRefGoogle Scholar
  40. 40.
    Dobosy JR, Selker EU (2001) Emerging connections between DNA methylation and histone acetylation. Cell Mol Life Sci 58:721–727PubMedCrossRefGoogle Scholar
  41. 41.
    Vaissiere T, Sawan C, Herceg Z (2008) Epigenetic interplay between histone modifications and DNA methylation in gene silencing. Mutat Res 659:40–48PubMedCrossRefGoogle Scholar
  42. 42.
    Ikegami K, Ohgane J, Tanaka S, Yagi S, Shiota K (2009) Interplay between DNA methylation, histone modification and chromatin remodeling in stem cells and during development. Int J Dev Biol 53:203–214PubMedGoogle Scholar
  43. 43.
    Tamaru H, Selker EU (2001) A histone H3 methyltransferase controls DNA methylation in Neurospora crassa. Nature 414:277–283PubMedCrossRefGoogle Scholar
  44. 44.
    Cosgrove DE, Cox GS (1990) Effects of sodium butyrate and 5-azacytidine on DNA methylation in human tumor cell lines: variable response to drug treatment and withdrawal. Biochim Biophys Acta 1087:80–86PubMedCrossRefGoogle Scholar
  45. 45.
    Hu JF, Oruganti H, Vu TH, Hoffman AR (1998) The role of histone acetylation in the allelic expression of the imprinted human insulin-like growth factor II gene. Biochem Biophys Res Commun 251:403–408PubMedCrossRefGoogle Scholar
  46. 46.
    Selker EU (1998) Trichostatin A causes selective loss of DNA methylation in Neurospora. Proc Natl Acad Sci USA 95:9430–9435PubMedCentralPubMedCrossRefGoogle Scholar
  47. 47.
    Cameron EE, Bachman KE, Myohanen S, Herman JG, Baylin SB (1999) Synergy of demethylation and histone deacetylase inhibition in the re-expression of genes silenced in cancer. Nat Genet 21:103–107PubMedCrossRefGoogle Scholar
  48. 48.
    El-Osta A, Kantharidis P, Zalcberg JR, Wolffe AP (2002) Precipitous release of methyl-CpG binding protein 2 and histone deacetylase 1 from the methylated human multidrug resistance gene (MDR1) on activation. Mol Cell Biol 22:1844–1857PubMedCentralPubMedCrossRefGoogle Scholar
  49. 49.
    Robert MF, Morin S, Beaulieu N, Gauthier F, Chute IC, Barsalou A, MacLeod AR (2003) DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells. Nat Genet 33:61–65PubMedCrossRefGoogle Scholar
  50. 50.
    Ng HH, Bird A (1999) DNA methylation and chromatin modification. Curr Opin Genet Dev 9:158–163PubMedCrossRefGoogle Scholar
  51. 51.
    Ballestar E, Wolffe AP (2001) Methyl-CpG-binding proteins. Targeting specific gene repression. Eur J Biochem 268:1–6PubMedCrossRefGoogle Scholar
  52. 52.
    Chen ZJ, Pikaard CS (1997) Epigenetic silencing of RNA polymerase I transcription: a role for DNA methylation and histone modification in nucleolar dominance. Genes Dev 11:2124–2136PubMedCentralPubMedCrossRefGoogle Scholar
  53. 53.
    Maass N, Biallek M, Rosel F, Schem C, Ohike N, Zhang M, Jonat W, Nagasaki K (2002) Hypermethylation and histone deacetylation lead to silencing of the maspin gene in human breast cancer. Biochem Biophys Res Commun 297:125–128PubMedCrossRefGoogle Scholar
  54. 54.
    Fournier C, Goto Y, Ballestar E, Delaval K, Hever AM, Esteller M, Feil R (2002) Allele-specific histone lysine methylation marks regulatory regions at imprinted mouse genes. EMBO J 21:6560–6570PubMedCentralPubMedCrossRefGoogle Scholar
  55. 55.
    Bannister AJ, Kouzarides T (2004) Histone methylation: recognizing the methyl mark. Methods Enzymol 376:269–288PubMedCrossRefGoogle Scholar
  56. 56.
    Schotta G, Lachner M, Peters AH, Jenuwein T (2004) The indexing potential of histone lysine methylation. Novartis Found Symp 259:22–37 (discussion 37–47, 163–169)PubMedCrossRefGoogle Scholar
  57. 57.
    Peters AH, Schubeler D (2005) Methylation of histones: playing memory with DNA. Curr Opin Cell Biol 17:230–238PubMedCrossRefGoogle Scholar
  58. 58.
    Kondo Y, Shen L, Issa JP (2003) Critical role of histone methylation in tumor suppressor gene silencing in colorectal cancer. Mol Cell Biol 23:206–215PubMedCentralPubMedCrossRefGoogle Scholar
  59. 59.
    Fujita H, Fujii R, Aratani S, Amano T, Fukamizu A, Nakajima T (2003) Antithetic effects of MBD2a on gene regulation. Mol Cell Biol 23:2645–2657PubMedCentralPubMedCrossRefGoogle Scholar
  60. 60.
    Li J, Lin Q, Yoon HG, Huang ZQ, Strahl BD, Allis CD, Wong J (2002) Involvement of histone methylation and phosphorylation in regulation of transcription by thyroid hormone receptor. Mol Cell Biol 22:5688–5697PubMedCentralPubMedCrossRefGoogle Scholar
  61. 61.
    Shi Y, Lan F, Matson C, Mulligan P, Whetstine JR, Cole PA, Casero RA, Shi Y (2004) Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 119:941–953PubMedCrossRefGoogle Scholar
  62. 62.
    Hendrich B, Bird A (1998) Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol 18:6538–6547PubMedCentralPubMedGoogle Scholar
  63. 63.
    Wade PA (2001) Methyl CpG-binding proteins and transcriptional repression. Bioessays 23:1131–1137PubMedCrossRefGoogle Scholar
  64. 64.
    Nan X, Bird A (2001) The biological functions of the methyl-CpG-binding protein MeCP2 and its implication in Rett syndrome. Brain Dev 23(Suppl 1):S32–S37PubMedCrossRefGoogle Scholar
  65. 65.
    Shahbazian MD, Antalffy B, Armstrong DL, Zoghbi HY (2002) Insight into Rett syndrome: MeCP2 levels display tissue- and cell-specific differences and correlate with neuronal maturation. Hum Mol Genet 11:115–124PubMedCrossRefGoogle Scholar
  66. 66.
    Hite KC, Adams VH, Hansen JC (2009) Recent advances in MeCP2 structure and function. Biochem Cell Biol 87:219–227PubMedCentralPubMedCrossRefGoogle Scholar
  67. 67.
    Urdinguio RG, Sanchez-Mut JV, Esteller M (2009) Epigenetic mechanisms in neurological diseases: genes, syndromes, and therapies. Lancet Neurol 8:1056–1072PubMedCrossRefGoogle Scholar
  68. 68.
    Brero A, Easwaran HP, Nowak D, Grunewald I, Cremer T, Leonhardt H, Cardoso MC (2005) Methyl CpG-binding proteins induce large-scale chromatin reorganization during terminal differentiation. J Cell Biol 169:733–743PubMedCentralPubMedCrossRefGoogle Scholar
  69. 69.
    Collins AL, Levenson JM, Vilaythong AP, Richman R, Armstrong DL, Noebels JL, Sweatt JD, Zoghbi HY (2004) Mild overexpression of MeCP2 causes a progressive neurological disorder in mice. Hum Mol Genet 13:2679–2689PubMedCrossRefGoogle Scholar
  70. 70.
    Bracaglia G, Conca B, Bergo A, Rusconi L, Zhou Z, Greenberg ME, Landsberger N, Soddu S, Kilstrup-Nielsen C (2009) Methyl-CpG-binding protein 2 is phosphorylated by homeodomain-interacting protein kinase 2 and contributes to apoptosis. EMBO Rep 10:1327–1333PubMedCentralPubMedCrossRefGoogle Scholar
  71. 71.
    Fujita N, Shimotake N, Ohki I, Chiba T, Saya H, Shirakawa M, Nakao M (2000) Mechanism of transcriptional regulation by methyl-CpG binding protein MBD1. Mol Cell Biol 20:5107–5118PubMedCentralPubMedCrossRefGoogle Scholar
  72. 72.
    Jorgensen HF, Ben-Porath I, Bird AP (2004) Mbd1 is recruited to both methylated and nonmethylated CpGs via distinct DNA binding domains. Mol Cell Biol 24:3387–3395PubMedCentralPubMedCrossRefGoogle Scholar
  73. 73.
    Nguyen CT, Weisenberger DJ, Velicescu M, Gonzales FA, Lin JC, Liang G, Jones PA (2002) Histone H3-lysine 9 methylation is associated with aberrant gene silencing in cancer cells and is rapidly reversed by 5-aza-2′-deoxycytidine. Cancer Res 62:6456–6461PubMedGoogle Scholar
  74. 74.
    Liang G, Lin JC, Wei V, Yoo C, Cheng JC, Nguyen CT, Weisenberger DJ, Egger G, Takai D, Gonzales FA, Jones PA (2004) Distinct localization of histone H3 acetylation and H3-K4 methylation to the transcription start sites in the human genome. Proc Natl Acad Sci USA 101:7357–7362PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Basel 2014

Authors and Affiliations

  • Yongli Chu
    • 1
  • Yanlin Wang
    • 2
  • Guanghua Zhang
    • 3
  • Haibin Chen
    • 4
  • Sean C. Dowdy
    • 5
  • Yuning Xiong
    • 5
  • Fengming Liu
    • 6
  • Run Zhang
    • 9
  • Jinping Li
    • 5
    • 7
    • 9
    Email author
  • Shi-Wen Jiang
    • 5
    • 7
    • 8
    • 9
    Email author
  1. 1.Yantai Yuhuangding Hospital Affiliated to Qingdao UniversityYantaiChina
  2. 2.Department of Reproductive MedicineBinzhou Medical University HospitalBinzhouChina
  3. 3.Tianjin Medical University Cancer HospitalTianjinChina
  4. 4.Department of Histology and EmbryologyShantou University Medical CollegeGuangdongChina
  5. 5.Department of Obstetrics and GynecologyMayo Clinic and Mayo Medical SchoolRochesterUSA
  6. 6.Department of Research and DevelopmentGuangxi Medicinal Botanical InstituteNanningChina
  7. 7.Curtis & Elizabeth Anderson Cancer InstituteMemorial Health University Medical CenterSavannahUSA
  8. 8.Department of Obstetrics and GynecologyMemorial Health University Medical CenterSavannahUSA
  9. 9.Department of Biomedical ScienceMercer University School of MedicineSavannahUSA

Personalised recommendations