Cellular and Molecular Life Sciences

, Volume 71, Issue 1, pp 113–142 | Cite as

The effect of five proteins on stem cells used for osteoblast differentiation and proliferation: a current review of the literature

  • P. Chatakun
  • R. Núñez-Toldrà
  • E. J. Díaz López
  • C. Gil-Recio
  • E. Martínez-Sarrà
  • F. Hernández-Alfaro
  • E. Ferrés-Padró
  • L. Giner-Tarrida
  • M. Atari
Review

Abstract

Bone-tissue engineering is a therapeutic target in the field of dental implant and orthopedic surgery. It is therefore essential to find a microenvironment that enhances the growth and differentiation of osteoblasts both from mesenchymal stem cells (MSCs) and those derived from dental pulp. The aim of this review is to determine the relationship among the proteins fibronectin (FN), osteopontin (OPN), tenascin (TN), bone sialoprotein (BSP), and bone morphogenetic protein (BMP2) and their ability to coat different types of biomaterials and surfaces to enhance osteoblast differentiation. Pre-treatment of biomaterials with FN during the initial phase of osteogenic differentiation on all types of surfaces, including slotted titanium and polymers, provides an ideal microenvironment that enhances adhesion, morphology, and proliferation of pluripotent and multipotent cells. Likewise, in the second stage of differentiation, surface coating with BMP2 decreases the diameter and the pore size of the scaffold, causing better adhesion and reduced proliferation of BMP-MSCs. Coating oligomerization surfaces with OPN and BSP promotes cell adhesion, but it is clear that the polymeric coating material BSP alone is insufficient to induce priming of MSCs and functional osteoblastic differentiation in vivo. Finally, TN is involved in mineralization and can accelerate new bone formation in a multicellular environment but has no effect on the initial stage of osteogenesis.

Keywords

Dental pulp stem cells Fibronectin BMPs Osteopontin Tenascin Bone sialoprotein 

Abbreviations

BMSCs

Bone marrow stromal cells

CaP

Calcium phosphate

ESCs

Embryonic stem cells

ECM

Extracellular matrix

FN

Fibronectin

HA

Hydroxyapatite

MSCs

Mesenchymal stem cells

PEA

Poly(ethyl acrylate)

PLLA

Poly(l-lactic acid)

TG

Tissue transglutaminase

ADSCs

Adipose-derived stromal cells

ASCs

Adult human adipose-derived stem cells

ALP

Alkaline phosphatase

BMPs

Bone morphogenetic proteins

FGF

Fibroblast growth factor

HGF

Hepatocyte growth factor

JNK

c-Jun NH2-terminal kinase

MMP

Metalloproteinase

OA

Osteoactivin

OC

Osteocalcin

PEMF

Pulsed electromagnetic fields

PDGF-AB

Platelet-derived growth factor-AB

PKA

cAMP-dependent protein kinase

PLGA

Poly(lactic-co-glycolic acid)

PTH

Parathyroid hormone

ROK

Rho-associated protein kinase

TGF-beta1

Transforming growth factor-beta1

VEGF

Vascular endothelial growth factor

BSP

Bone sialoprotein

ERRalpha

Estrogen receptor-related receptor alpha

GM-CSF

Granulocyte macrophage colony-stimulating factor

OPN

Osteopontin

BMSC

Bone mesenchymal stem cell

AP1

Activator protein 1

CRE

cAMP response element

FGF2

Fibroblast growth factor 2

FRE

Fibroblast growth factor 2 response element

FSK

Forskolin

HOX

Homeodomain protein-binding site

LPS

Lipopolysaccharide

PDGF

Platelet-derived growth factor

PCL/pHEMA

Polycaprolactone/poly(2-hydroxyethyl methacrylate

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Copyright information

© Springer Basel 2013

Authors and Affiliations

  • P. Chatakun
    • 1
    • 2
  • R. Núñez-Toldrà
    • 1
    • 3
  • E. J. Díaz López
    • 1
  • C. Gil-Recio
    • 1
    • 3
  • E. Martínez-Sarrà
    • 1
    • 3
  • F. Hernández-Alfaro
    • 4
  • E. Ferrés-Padró
    • 4
    • 5
  • L. Giner-Tarrida
    • 1
    • 3
  • M. Atari
    • 1
    • 3
    • 4
  1. 1.Laboratory for Regenerative Medicine, College of DentistryUniversitat Internacional de CatalunyaBarcelonaSpain
  2. 2.Police General HospitalBangkokThailand
  3. 3.Chair of Regenerative Implantology MIS-UICUniversitat Internacional de CatalunyaBarcelonaSpain
  4. 4.Surgery and Oral Implantology Department, College of DentistryUniversitat Internacional de CatalunyaBarcelonaSpain
  5. 5.Oral and Maxillofacial Surgery DepartmentFundacio Hospital de Nens de BarcelonaBarcelonaSpain

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