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Cellular and Molecular Life Sciences

, Volume 69, Issue 23, pp 3989–3997 | Cite as

A matricellular protein and EGF-like repeat signalling in the social amoebozoan Dictyostelium discoideum

  • Robert J. HuberEmail author
  • Danton H. O’Day
Review

Abstract

Matricellular proteins interact with the extracellular matrix (ECM) and modulate cellular processes by binding to cell surface receptors and initiating intracellular signal transduction. Their association with the ECM and the ability of some members of this protein family to regulate cell motility have opened up new avenues of research to investigate their functions in normal and diseased cells. In this review, we summarize the research on CyrA, an ECM calmodulin-binding protein in Dictyostelium. CyrA is proteolytically cleaved into smaller EGF-like (EGFL) repeat containing cleavage products during development. The first EGFL repeat of CyrA binds to the cell surface and activates a novel signalling pathway that modulates cell motility in this model organism. The similarity of CyrA to the most well-characterized matricellular proteins in mammals allows it to be designated as the first matricellular protein identified in Dictyostelium.

Keywords

Matricellular EGF-like repeat Extracellular matrix Signal transduction Cell motility Dictyostelium CyrA 

Abbreviations

AcbA

Acyl-CoA binding protein A

ADAMTS

A disintegrin and mettaloproteinase with thrombospondin motifs

carA

cAMP receptor A

carC

cAMP receptor C

CaM

Calmodulin

CaMBP

CaM-binding protein

CyrA

Cysteine-rich protein A

CyrA-C40

40 kDa CyrA cleavage product

CyrA-C45

45 kDa CyrA cleavage product

ECM

Extracellular matrix

EGFL

Epidermal growth factor-like

EGFL1

CyrA EGFL repeat 1

EGFR

EGF receptor

ER

Endoplasmic reticulum

MHC

Myosin II heavy chain

MMP2

Matrix metalloproteinase 2

PaxB

Paxillin B

PI3K

Phosphatidylinositol-3-kinase

PKA

Protein kinase A

PLA2

Phospholipase A2

PLC

Phospholipase C

SDF-2

Spore differentiation factor 2

SPARC

Secreted protein acidic and rich in cysteine

TalB

Talin B

Ten14

14th EGFL repeat of tenascin C

Tenascin C

Tenascin cytotactin

TSP

Thrombospondin

VinB

Vinculin B

Notes

Acknowledgments

This review was supported by a Discovery Grant (D.H.O’D.; A6807) and a Canada Graduate Scholarship (R.J.H.) from the Natural Sciences and Engineering Research Council of Canada.

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© Springer Basel AG 2012

Authors and Affiliations

  1. 1.Department of Cell and Systems BiologyUniversity of TorontoTorontoCanada
  2. 2.Department of BiologyUniversity of Toronto MississaugaMississaugaCanada

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