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Cellular and Molecular Life Sciences

, Volume 69, Issue 10, pp 1689–1703 | Cite as

Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell tracking and traction force microscopy

  • Sara Nocentini
  • Diego Reginensi
  • Simón Garcia
  • Patricia Carulla
  • María Teresa Moreno-Flores
  • Francisco Wandosell
  • Xavier Trepat
  • Ana Bribian
  • José A. del Río
Research Article

Abstract

Newly generated olfactory receptor axons grow from the peripheral to the central nervous system aided by olfactory ensheathing cells (OECs). Thus, OEC transplantation has emerged as a promising therapy for spinal cord injuries and for other neural diseases. However, these cells do not present a uniform population, but instead a functionally heterogeneous population that exhibits a variety of responses including adhesion, repulsion, and crossover during cell–cell and cell–matrix interactions. Some studies report that the migratory properties of OECs are compromised by inhibitory molecules and potentiated by chemical gradients. Here, we demonstrated that rodent OECs express all the components of the Nogo receptor complex and that their migration is blocked by myelin. Next, we used cell tracking and traction force microscopy to analyze OEC migration and its mechanical properties over myelin. Our data relate the decrease of traction force of OEC with lower migratory capacity over myelin, which correlates with changes in the F-actin cytoskeleton and focal adhesion distribution. Lastly, OEC traction force and migratory capacity is enhanced after cell incubation with the Nogo receptor inhibitor NEP1-40.

Keywords

Ensheathing glia Traction force microscopy Migration Myelin-associated inhibitors 

Notes

Acknowledgments

The authors thank R. Rycroft for linguistic advice and G. Tormen for technical assistance. This work was supported by FP7-PRIORITY, the MICINN (BFU2009-10848) and grants from the Instituto Carlos III (PI11/03028) and SGR2009-366 (Generalitat of Catalunya) to JADR. SN and PC were supported by the MICINN. DR is supported by a fellowship from the National Commission for Science and Technology (CONICYT, Chile). AB is a Sara Borrell postdoctoral research of the Instituto Carlos III. XT acknowledges support of the Spanish Ministry for Science and Innovation (BFU2009-07595) and the European Research Council (Grant Agreement 242993). F. Wandosell was supported by MICINN (SAF2009-12249-C02-01).

Supplementary material

18_2011_893_MOESM1_ESM.tif (2 mb)
Fig. S1. Myelin treatment activates RhoA and induces ERK1-2 phosphorylation in TEG3 cells. Time course of RhoA activation (a) and ERK1-2 phosphorylation (b) in TEG3 cells after myelin treatment. See the sections Materials and methods and Results for details (TIFF 2036 kb)

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Copyright information

© Springer Basel AG 2011

Authors and Affiliations

  • Sara Nocentini
    • 1
    • 2
    • 3
  • Diego Reginensi
    • 1
    • 2
    • 3
  • Simón Garcia
    • 4
  • Patricia Carulla
    • 1
    • 2
    • 3
  • María Teresa Moreno-Flores
    • 8
  • Francisco Wandosell
    • 8
    • 9
  • Xavier Trepat
    • 4
    • 5
    • 6
    • 7
  • Ana Bribian
    • 1
    • 2
    • 3
  • José A. del Río
    • 1
    • 2
    • 3
  1. 1.Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), Barcelona Science ParkUniversity of BarcelonaBarcelonaSpain
  2. 2.Department of Cell Biology, Faculty of BiologyUniversity of BarcelonaBarcelonaSpain
  3. 3.Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED)BarcelonaSpain
  4. 4.Integrative Cell and Tissue Dynamics, Institute for Bioengineering of Catalonia (IBEC), Barcelona Science ParkUniversity of BarcelonaBarcelonaSpain
  5. 5.Institució Catalana de Recerca i Estudis Avançats (ICREA)BarcelonaSpain
  6. 6.Facultat de MedicinaUniversitat de BarcelonaBarcelonaSpain
  7. 7.Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES)BarcelonaSpain
  8. 8.Centro de Biología Molecular “Severo Ochoa”, Nicolás Cabrera, 1Universidad Autónoma de Madrid (CBM-UAM)MadridSpain
  9. 9.Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), CBM-UAMMadridSpain

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