Cellular and Molecular Life Sciences

, Volume 68, Issue 12, pp 2101–2114 | Cite as

Wnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow

  • A. Glejzer
  • E. Laudet
  • P. Leprince
  • B. Hennuy
  • C. Poulet
  • O. Shakhova
  • L. Sommer
  • B. Rogister
  • S. Wislet-GendebienEmail author
Research Article


Recent studies have shown that neural crest-derived progenitor cells can be found in diverse mammalian tissues including tissues that were not previously shown to contain neural crest derivatives, such as bone marrow. The identification of those "new" neural crest-derived progenitor cells opens new strategies for developing autologous cell replacement therapies in regenerative medicine. However, their potential use is still a challenge as only few neural crest-derived progenitor cells were found in those new accessible locations. In this study, we developed a protocol, based on wnt1 and BMP2 effects, to enrich neural crest-derived cells from adult bone marrow. Those two factors are known to maintain and stimulate the proliferation of embryonic neural crest stem cells, however, their effects have never been characterized on neural crest cells isolated from adult tissues. Using multiple strategies from microarray to 2D-DIGE proteomic analyses, we characterized those recruited neural crest-derived cells, defining their identity and their differentiating abilities.


Neural crest Bone marrow wnt1 BMP2 Multipotent progenitors 



Bone morphogenic proteins


Bone marrow stromal cells


Embryonic stem cells


Glial fibrillary acidic protein


Mesenchymal stem cells


Neural crest cells


Neural crest stem cells


Neural stem cells



This work was supported by a grant from the Fonds National de la Recherche Scientifique (FNRS) of Belgium, by a grant of the Action de Recherche Concertée de la Communauté Française de Belgique, and by the Belgian League against Multiple Sclerosis associated with Leon Fredericq Foundation. AG is a Marie Curie Host Fellow for Early Stage Research Training, EURON 020589 within the 6th FP of the EU, Marie Curie Actions, Human Resources and Mobility. SWG is a Postdoctoral researcher and PL is a Research Associate of the FNRS. Normalization and data filtering were performed using BRB-ArrayTools software version 3.8.1 developed by Dr. Richard Simons and BRB-ArrayTools Development Team

Supplementary material

18_2010_558_MOESM1_ESM.doc (1.2 mb)
Table S1. Gene expression profile comparison between clone 1 and clone 4. 328 genes have been identified as differentially expressed between clone 1 and 4, with a minimum of 2 fold difference
18_2010_558_MOESM2_ESM.doc (4.1 mb)
Table S2. Gene expression profile comparison between clone 1 and NCSC clone (wnt1-CRE/R26R). 3,578 genes have been identified as differentially expressed between clone 1 and 4, with a minimum of 2 fold difference
18_2010_558_MOESM3_ESM.doc (7.3 mb)
Table S3. Gene expression profile comparison between clone 4 and NCSC clone (wnt1-CRE/R26R). 3966 genes have been identified as differentially expressed between clone 4 and NCSC clone, with a minimum of 2 fold difference
18_2010_558_MOESM4_ESM.doc (375 kb)
Table S4. Gene expression profile of specific cell type genes: comparison between clone 1 and clone 4


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Copyright information

© Springer Basel AG 2010

Authors and Affiliations

  • A. Glejzer
    • 1
  • E. Laudet
    • 1
  • P. Leprince
    • 1
  • B. Hennuy
    • 2
  • C. Poulet
    • 2
  • O. Shakhova
    • 3
  • L. Sommer
    • 3
  • B. Rogister
    • 1
    • 4
    • 5
  • S. Wislet-Gendebien
    • 1
    Email author
  1. 1.GIGA NeurosciencesUniversity of LiegeLiègeBelgium
  2. 2.University of LiegeLiègeBelgium
  3. 3.Institute of Anatomy, University of ZurichZurichSwitzerland
  4. 4.GIGA Development, Stem Cells and Regenerative MedicineUniversity of LiègeLiègeBelgium
  5. 5.Department of NeurologyUniversity of LiègeLiègeBelgium

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