Cellular and Molecular Life Sciences

, Volume 68, Issue 12, pp 2101–2114 | Cite as

Wnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow

  • A. Glejzer
  • E. Laudet
  • P. Leprince
  • B. Hennuy
  • C. Poulet
  • O. Shakhova
  • L. Sommer
  • B. Rogister
  • S. Wislet-Gendebien
Research Article

Abstract

Recent studies have shown that neural crest-derived progenitor cells can be found in diverse mammalian tissues including tissues that were not previously shown to contain neural crest derivatives, such as bone marrow. The identification of those "new" neural crest-derived progenitor cells opens new strategies for developing autologous cell replacement therapies in regenerative medicine. However, their potential use is still a challenge as only few neural crest-derived progenitor cells were found in those new accessible locations. In this study, we developed a protocol, based on wnt1 and BMP2 effects, to enrich neural crest-derived cells from adult bone marrow. Those two factors are known to maintain and stimulate the proliferation of embryonic neural crest stem cells, however, their effects have never been characterized on neural crest cells isolated from adult tissues. Using multiple strategies from microarray to 2D-DIGE proteomic analyses, we characterized those recruited neural crest-derived cells, defining their identity and their differentiating abilities.

Keywords

Neural crest Bone marrow wnt1 BMP2 Multipotent progenitors 

Abbreviations

BMP

Bone morphogenic proteins

BMSC

Bone marrow stromal cells

ES

Embryonic stem cells

GFAP

Glial fibrillary acidic protein

MSC

Mesenchymal stem cells

NCC

Neural crest cells

NCSC

Neural crest stem cells

NSC

Neural stem cells

Supplementary material

18_2010_558_MOESM1_ESM.doc (1.2 mb)
Table S1. Gene expression profile comparison between clone 1 and clone 4. 328 genes have been identified as differentially expressed between clone 1 and 4, with a minimum of 2 fold difference
18_2010_558_MOESM2_ESM.doc (4.1 mb)
Table S2. Gene expression profile comparison between clone 1 and NCSC clone (wnt1-CRE/R26R). 3,578 genes have been identified as differentially expressed between clone 1 and 4, with a minimum of 2 fold difference
18_2010_558_MOESM3_ESM.doc (7.3 mb)
Table S3. Gene expression profile comparison between clone 4 and NCSC clone (wnt1-CRE/R26R). 3966 genes have been identified as differentially expressed between clone 4 and NCSC clone, with a minimum of 2 fold difference
18_2010_558_MOESM4_ESM.doc (375 kb)
Table S4. Gene expression profile of specific cell type genes: comparison between clone 1 and clone 4

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Copyright information

© Springer Basel AG 2010

Authors and Affiliations

  • A. Glejzer
    • 1
  • E. Laudet
    • 1
  • P. Leprince
    • 1
  • B. Hennuy
    • 2
  • C. Poulet
    • 2
  • O. Shakhova
    • 3
  • L. Sommer
    • 3
  • B. Rogister
    • 1
    • 4
    • 5
  • S. Wislet-Gendebien
    • 1
  1. 1.GIGA NeurosciencesUniversity of LiegeLiègeBelgium
  2. 2.University of LiegeLiègeBelgium
  3. 3.Institute of Anatomy, University of ZurichZurichSwitzerland
  4. 4.GIGA Development, Stem Cells and Regenerative MedicineUniversity of LiègeLiègeBelgium
  5. 5.Department of NeurologyUniversity of LiègeLiègeBelgium

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