Wnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow
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Recent studies have shown that neural crest-derived progenitor cells can be found in diverse mammalian tissues including tissues that were not previously shown to contain neural crest derivatives, such as bone marrow. The identification of those "new" neural crest-derived progenitor cells opens new strategies for developing autologous cell replacement therapies in regenerative medicine. However, their potential use is still a challenge as only few neural crest-derived progenitor cells were found in those new accessible locations. In this study, we developed a protocol, based on wnt1 and BMP2 effects, to enrich neural crest-derived cells from adult bone marrow. Those two factors are known to maintain and stimulate the proliferation of embryonic neural crest stem cells, however, their effects have never been characterized on neural crest cells isolated from adult tissues. Using multiple strategies from microarray to 2D-DIGE proteomic analyses, we characterized those recruited neural crest-derived cells, defining their identity and their differentiating abilities.
KeywordsNeural crest Bone marrow wnt1 BMP2 Multipotent progenitors
Bone morphogenic proteins
Bone marrow stromal cells
Embryonic stem cells
Glial fibrillary acidic protein
Mesenchymal stem cells
Neural crest cells
Neural crest stem cells
Neural stem cells
This work was supported by a grant from the Fonds National de la Recherche Scientifique (FNRS) of Belgium, by a grant of the Action de Recherche Concertée de la Communauté Française de Belgique, and by the Belgian League against Multiple Sclerosis associated with Leon Fredericq Foundation. AG is a Marie Curie Host Fellow for Early Stage Research Training, EURON 020589 within the 6th FP of the EU, Marie Curie Actions, Human Resources and Mobility. SWG is a Postdoctoral researcher and PL is a Research Associate of the FNRS. Normalization and data filtering were performed using BRB-ArrayTools software version 3.8.1 developed by Dr. Richard Simons and BRB-ArrayTools Development Team http://linus.nci.nih.gov./BRB-ArrayTools.html.
- 11.Wong CE, Paratore C, Dours-Zimmermann MT, Rochat A, Pietri T, Suter U, Zimmermann DR, Dufour S, Thiery JP, Meijer D, Beermann F, Barrandon Y, Sommer L (2006) Neural crest-derived cells with stem cell features can be traced back to multiple lineages in the adult skin. J Cell Biol 175:1005–1015PubMedCrossRefGoogle Scholar
- 12.Nagoshi N, Shibata S, Kubota Y, Nakamura M, Nagai Y, Satoh E, Morikawa S, Okada Y, Mabuchi Y, Katoh H, Okada S, Fukuda K, Suda T, Matsuzaki Y, Toyama Y, Okano H (2008) Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad. Cell Stem Cell 2:392–403PubMedCrossRefGoogle Scholar