Cellular and Molecular Life Sciences

, Volume 67, Issue 23, pp 4049–4064 | Cite as

Reconstruction of gene association network reveals a transmembrane protein required for adipogenesis and targeted by PPARγ

  • Juliane G. Bogner-Strauss
  • Andreas Prokesch
  • Fatima Sanchez-Cabo
  • Dietmar Rieder
  • Hubert Hackl
  • Kalina Duszka
  • Anne Krogsdam
  • Barbara Di Camillo
  • Evelyn Walenta
  • Ariane Klatzer
  • Achim Lass
  • Montserrat Pinent
  • Wing-Cheong Wong
  • Frank Eisenhaber
  • Zlatko Trajanoski
Research Article

Abstract

We have developed a method for reconstructing gene association networks and have applied this method to gene profiles from 3T3-L1 cells. Priorization of the candidate genes pinpointed a transcript annotated as APMAP (adipocyte plasma membrane-associated protein). Functional studies showed that APMAP is upregulated in murine and human adipogenic cell models as well as in a genetic mouse model of obesity. Silencing APMAP in 3T3-L1 cells strongly impaired the differentiation into adipocytes. Moreover, APMAP expression was strongly induced by the PPARγ ligand rosiglitazone in adipocytes in vitro and in vivo in adipose tissue. Using ChIP-qPCR and luciferase reporter assays, we show a functional PPARγ binding site. In addition, we provide evidence that the extracellular C-terminal domain of APMAP is required for the function of APMAP in adipocyte differentiation. Finally, we demonstrate that APMAP translocates from the endoplasmatic reticulum to the plasma membrane during adipocyte differentiation.

Keywords

Adipogenesis APMAP PPARγ Transcriptional regulation Gene expression Gene networks 

Supplementary material

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Copyright information

© Springer Basel AG 2010

Authors and Affiliations

  • Juliane G. Bogner-Strauss
    • 1
  • Andreas Prokesch
    • 1
  • Fatima Sanchez-Cabo
    • 2
  • Dietmar Rieder
    • 1
  • Hubert Hackl
    • 1
  • Kalina Duszka
    • 1
  • Anne Krogsdam
    • 1
  • Barbara Di Camillo
    • 3
  • Evelyn Walenta
    • 1
  • Ariane Klatzer
    • 1
  • Achim Lass
    • 4
  • Montserrat Pinent
    • 1
  • Wing-Cheong Wong
    • 5
  • Frank Eisenhaber
    • 5
    • 6
    • 7
  • Zlatko Trajanoski
    • 8
  1. 1.Institute for Genomics and BioinformaticsGraz University of TechnologyGrazAustria
  2. 2.Genomics UnitCentro Nacional de Investigaciones Cardiovasculares (CNIC)MadridSpain
  3. 3.Information Engineering DepartmentUniversity of PadovaPadovaItaly
  4. 4.Institute for Molecular BiosciencesKarl-Franzens University GrazGrazAustria
  5. 5.Bioinformatics Institute (BII)Agency for Science, Technology and Research (A*STAR)SingaporeSingapore
  6. 6.Department of Biological Sciences (DBS)National University of Singapore (NUS)SingaporeSingapore
  7. 7.School of Computer Engineering (SCE)Nanyang Technological University (NTU)SingaporeSingapore
  8. 8.Biocenter, Section for BioinformaticsInnsbruck Medical UniversityInnsbruckAustria

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