Identification of cancer stem cell-like cells from human epithelial ovarian carcinoma cell line
- 573 Downloads
Cancer stem cells (CSCs) play an important role in the development, invasion, and drug resistance of carcinoma, but the exact phenotype and characteristics of ovarian CSCs are still disputable. In this study, we identified cancer stem cell-like cells (CSC-LCs) and investigated their characteristics from the ovarian adenocarcinoma cell line 3AO. Our results showed that CSC-LCs were enriched in sphere-forming test and highly expressed CD44+CD24−. The spheres and CD24− cells possessed strong tumorigenic ability by transplantation into nonobese diabetic/severe combined immunodeficient mice. CD44+CD24− cells expressed stem cell markers and differentiated to CD44+CD24+ cells by immunofluorescence assay and fluorescence-activated cell-sorting analysis. In vitro experiments verified that CD44+CD24− cells were markedly resistant to carboplatin and paclitaxol. In conclusion, our study identifies the CD44+CD24− phenotype, self-renewal, high tumorigenicity, differentiation potential, and drug resistance of ovarian CSC-LCs. Our findings may provide the evidence needed to explore a new strategy in the treatment of ovarian cancer.
KeywordsCancer stem cells Ovarian carcinoma CD44 CD24
This research was supported by the National Natural Science Foundation of China (Grant No. 30672230), the National Program on Key Basic Research Project of China (Grant No. 2009CB521800), and the Foundation of Health Department of Zhejiang Province of China (Grant No. 2007A129). We thank Professor Li ZT (Cancer Hospital of Fudan University), Mrs. Zhou J (Shanghai Cancer Institute, Shanghai Jiao Tong University), Mrs. Xu HL (Shanghai Cancer Institute, Shanghai Jiao Tong University), and Mr. Geng Q (Shanghai Cancer Institute, Shanghai Jiao Tong University), for technical assistance.
- 41.Yoshida J, Horiuchi A, Kikuchi N, Hayashi A, Osada R, Ohira S, Shiozawa T, Konishi I (2009) Changes in the expression of E-cadherin repressors, Snail, Slug, SIP1, and Twist, in the development and progression of ovarian carcinoma: the important role of Snail in ovarian tumorigenesis and progression. Med Mol Morphol 42:82–91CrossRefPubMedGoogle Scholar