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Cellular and Molecular Life Sciences

, Volume 67, Issue 15, pp 2643–2651 | Cite as

Expression of defensins in non-infected araneomorph spiders

  • Tommy Baumann
  • Lucia Kuhn-NentwigEmail author
  • Carlo R. Largiadèr
  • Wolfgang Nentwig
Research Article

Abstract

Defensins are a major family of antimicrobial peptides found throughout the phylogenetic tree. From the spider species: Cupiennius salei, Phoneutria reidyi, Polybetes pythagoricus, Tegenaria atrica, and Meta menardi, defensins belonging to the ‘ancestral’ class of invertebrate defensins were cloned and sequenced. The deduced amino acid sequences contain the characteristic six cysteines of this class of defensins and reveal precursors of 60 or 61 amino acid residues. The mature peptides consist of 37 amino acid residues, showing up to 70% identities with tick and scorpion defensins. In C. salei, defensin mRNA was found to be constitutively expressed in hemocytes, ovaries, subesophageal nerve mass, hepatopancreas, and muscle tissue. This is the first report presenting and comparing antimicrobial peptides belonging to the family of defensins from spiders.

Keywords

Spider defensins Cupiennius salei Phoneutria reidyi Polybetes pythagoricus Tegenaria atrica Meta menardi Tissue expression 

Notes

Acknowledgments

We thank Dr. C. Kropf for kindly providing M. menardi, Prof. Dr. J. Schaller for MS measurements, Dr. D. Destoumieux-Garzón and Dr. H. Murray for critical comments on the manuscript, and the Swiss National Science Foundation for funding.

Supplementary material

18_2010_354_MOESM1_ESM.tif (4.6 mb)
Fig. S1 Partial C. salei defensin gene structure. PCR products of hemocyte cDNA and genomic DNA with defensin-specific primers. The 50-bp molecular weight marker is marked with MW; band sizes are in bp. (TIFF 4753 kb)
18_2010_354_MOESM2_ESM.doc (50 kb)
Supplementary material (DOC 50 kb)

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Copyright information

© Springer Basel AG 2010

Authors and Affiliations

  • Tommy Baumann
    • 1
  • Lucia Kuhn-Nentwig
    • 1
    Email author
  • Carlo R. Largiadèr
    • 2
  • Wolfgang Nentwig
    • 1
  1. 1.Institute of Ecology and EvolutionUniversity of BernBernSwitzerland
  2. 2.Institute of Clinical Chemistry, Bern University HospitalUniversity of BernBernSwitzerland

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