Cellular and Molecular Life Sciences

, Volume 66, Issue 7, pp 1309–1319 | Cite as

Activation of hypoxia-inducible factor-1 regulates human histidine decarboxylase expression

  • H. J. Jeong
  • P. D. Moon
  • S. J. Kim
  • J. U. Seo
  • T. H. Kang
  • J. J. Kim
  • I. C. Kang
  • J. Y. Um
  • H. M. Kim
  • S. H. Hong
Research Article

Abstract.

Histidine decarboxylase (HDC) catalyzes the formation of histamine from histidine. Histamine has various effects in physiological and pathological reactions, such as inflammation, cell growth, and neuro-transmission. We investigated the role of hypoxia-inducible factor (HIF)-1 on hypoxia-induced HDC expression in human mast cell line, HMC-1 cells and mouse bone marrow-derived mast cells (BMMCs). Hypoxia significantly increased histamine production. HDC expression and activity were induced by hypoxia. Additionally, when cells were transfected with a native form of HIF-1α, hypoxia could induce higher HDC expression than in the nontransfected cell. HIF-1 binding activity for HDC 5’ flanking region (HFR) was similar to that for the hypoxia-responsive element. Using HDC promoter deletion analysis, we also demonstrated that HFR was regulated by HIF-1 activation. In addition, depletion of HIF-1α prevents hypoxic induction of HDC in BMMCs. In conclusion, these results demonstrate that hypoxia induces HDC expression by transcriptional mechanisms dependent upon HIF-1.

Keywords.

Histidine decarboxylase hypoxia mast cell hypoxia inducible factor-1 histamine 

Copyright information

© Birkhäuser Verlag, Basel 2009

Authors and Affiliations

  • H. J. Jeong
    • 1
  • P. D. Moon
    • 2
  • S. J. Kim
    • 2
    • 3
  • J. U. Seo
    • 4
  • T. H. Kang
    • 4
  • J. J. Kim
    • 4
  • I. C. Kang
    • 1
  • J. Y. Um
    • 2
  • H. M. Kim
    • 2
  • S. H. Hong
    • 3
  1. 1.Biochip Research CenterHoseo UniversityChungnamRepublic of Korea
  2. 2.Department of Pharmacology, Institute of Oriental Medicine, College of Oriental MedicineKyung Hee UniversitySeoulRepublic of Korea
  3. 3.College of PharmacyVestibuloCochlear Research Center of Wonkwang UniversityIksan, JeonbukRepublic of Korea
  4. 4.Cancer Preventive Material Development Research Center, Department of Pharmacology, Institute of Oriental Medicine, College of Oriental MedicineKyung Hee UniversitySeoulRepublic of Korea

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