Cellular and Molecular Life Sciences

, Volume 67, Issue 3, pp 455–466

Lucifensin, the long-sought antimicrobial factor of medicinal maggots of the blowfly Lucilia sericata

  • Václav Čeřovský
  • Jan Žďárek
  • Vladimír Fučík
  • Lenka Monincová
  • Zdeněk Voburka
  • Robert Bém
Research Article

DOI: 10.1007/s00018-009-0194-0

Cite this article as:
Čeřovský, V., Žďárek, J., Fučík, V. et al. Cell. Mol. Life Sci. (2010) 67: 455. doi:10.1007/s00018-009-0194-0

Abstract

A novel homologue of insect defensin designated lucifensin (Lucilia defensin) was purified from the extracts of various tissues (gut, salivary glands, fat body, haemolymph) of green bottle fly (Luciliasericata) larvae and from their excretions/secretions. The primary sequence of this peptide of 40 residues and three intramolecular disulfide bridges was determined by ESI-QTOF mass spectrometry and Edman degradation and is very similar to that of sapecin and other dipteran defensins. We assume that lucifensin is the key antimicrobial component that protects the maggots when they are exposed to the highly infectious environment of a wound during the medicinal process known as maggot therapy. We also believe that lucifensin is that long-sought larger molecular weight antimicrobial factor of the Lucilia sericata excretions/secretions believed to be effective against pathogenic elements of the wound microbial flora.

Keywords

Antimicrobial peptide Insect defensin Maggot therapy Lucilia sericata 

Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2009

Authors and Affiliations

  • Václav Čeřovský
    • 1
  • Jan Žďárek
    • 1
  • Vladimír Fučík
    • 1
  • Lenka Monincová
    • 1
  • Zdeněk Voburka
    • 1
  • Robert Bém
    • 2
  1. 1.Institute of Organic Chemistry and BiochemistryAcademy of Sciences of the Czech RepublicPrague 6Czech Republic
  2. 2.Diabetes CentreInstitute for Clinical and Experimental MedicinePrague 4Czech Republic

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