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Cellular and Molecular Life Sciences

, Volume 66, Issue 24, pp 3837–3850 | Cite as

Ecdysteroid hormone action

  • Klaus-Dieter Spindler
  • C. Hönl
  • Ch. Tremmel
  • S. Braun
  • H. Ruff
  • M. Spindler-Barth
Review

Abstract

Several reviews devoted to various aspects of ecdysone research have been published during the last few years. Therefore, this article concentrates mainly on the considerable progress in ecdysone research observed recently, and will cover the results obtained during the last 2 years. The main emphasis is put on the molecular mode of ecdysteroid receptor-mediated hormone action. Two examples of interaction with other hormonal signalling pathways are described, namely crosstalk with juvenile hormone and insulin. Some selected, recently investigated examples of the multitude of hormonal responses are described. Finally, ecological aspects and some practical applications are discussed.

Keywords

Ecdysone Insulin Juvenile hormone Nuclear receptor Ultraspiracle 

References

  1. 1.
    Litwack G (2005) Insect hormones. Vitam Horm 73:1–288Google Scholar
  2. 2.
    Smagghe G (2009) Ecdysones, structures and functions. Springer, BerlinGoogle Scholar
  3. 3.
    Spindler K-D, Przibilla S, Spindler-Barth M (2001) Moulting hormones of arthropods: molecular mechanisms. Zoology 103:189–201Google Scholar
  4. 4.
    Dinan L (2009) Phytoecdysteroids: what use are they? Arch. Insect Biochem Physiol (in press)Google Scholar
  5. 5.
    Dinan L, Harmatha J, Volodin V, Lafont R (2009) Phytoecdysteroids: diversity, biosynthesis and distribution. In: Smagghe G (ed) Ecdysteroids, structures and functions. Springer, Berlin, pp 3–45Google Scholar
  6. 6.
    Gilbert LI (2008) Drosophila is an inclusive model for human diseases, growth and development. Mol Cell Endocrinol 293:25–31PubMedGoogle Scholar
  7. 7.
    Baker KD, Thummel CS (2007) Diabetic larvae and obese flies—emerging studies of metabolism in Drosophila. Cell Metab 6:257–266PubMedGoogle Scholar
  8. 8.
    Palli SR, Hormann RE, Schlattner U, Lezzi M (2005) Ecdysteroid receptors and their applications in agriculture and medicine. Vitam Horm 73:60–100Google Scholar
  9. 9.
    Palli SR, Taava VS (2009) Ecdysone receptor gene switches for applications in medicine and agriculture. In: Smagghe G (ed) Ecdysone: structures and functions. Springer, Berlin, pp 551–538Google Scholar
  10. 10.
    Rangasamy D, Tremethick DJ, Greaves IK (2008) Gene knockdown by ecdysone-based inducible RNAi in stable mammalian cell lines. Nat Protoc 3:79–88PubMedGoogle Scholar
  11. 11.
    Lafont R, Dinan L (2003) Practical uses for ecdsteroids in mammals including humans: an update. J Insect Sci 3.7:1–30Google Scholar
  12. 12.
    Huang X, Warren JT, Gilbert LI (2008) New players in the regulation of ecdysone biosynthesis. J Genet Genomics 35:1–10PubMedGoogle Scholar
  13. 13.
    Rodenburg KW, van der Horst DJ (2005) Lipoprotein-mediated lipid transport in insects: analogy to mammalian lipid carrier system and novel concepts for the functioning of LDL receptor family members. Biochim Biophys Acta 1736:10–29PubMedGoogle Scholar
  14. 14.
    Whitehead L (1989) Ecdysteroid carrier proteins. In: Koolman J (ed) Ecdysone—from chemistry to mode of action. Thieme, Stuttgart, pp 232–244Google Scholar
  15. 15.
    Jänicke E, Föll R, Decker H (1999) Spider hemocyanin binds ecdysone and 20-OH-ecdysone. J Biol Chem 274:34267–34271Google Scholar
  16. 16.
    Riddiford LM, Cherbas P, Truman JW (2000) Ecdysone receptors and their biological actions. Vitam Horm 60:1–73PubMedGoogle Scholar
  17. 17.
    Truman JW (2005) Hormonal control of insect ecdysis; endocrine cascades for coordinating behavior with physiology. Vitam Horm 73:1–30PubMedGoogle Scholar
  18. 18.
    Zitnan D, Kim YJ, Zitnanová I, Roller L, Adams ME (2007) Complex steroid–peptide–receptor cascade controls insect ecdysis. Gen Comp Endocrinol 153:88–96PubMedGoogle Scholar
  19. 19.
    De Loof A (2008) Ecdysteroids, juvenile hormone and insect neuropeptides: recent success and remaining major challenges. Gen Comp Endocrinol 155:3–13PubMedGoogle Scholar
  20. 20.
    Henrich VC (2005) The ecdysteroid receptor In: Iatrou K, Gill SS (eds) Comprehensive insect physiology, biochemistry, and molecular biology series, vol 3. Elsevier, Oxford, pp 243–286Google Scholar
  21. 21.
    Spindler-Barth M, Spindler K-D (2003) Ecdysteroid receptors (EcR/USP). In: Henry HL, Norman AW (eds) Encyclopedia of hormones and related cell regulators. Academic Press, London, pp 466–470Google Scholar
  22. 22.
    Braun S, Azoitei A, Spindler-Barth M (2009) DNA binding properties of Drosophila ecdysone receptor isoforms and its modification by the heterodimerization partner ultraspiracle. Arch Insect Biochem Physiol (in press)Google Scholar
  23. 23.
    Cronauer MV, Braun S, Tremmel Ch, Kröncke KD, Spindler-Barth M (2007) Nuclear localization and DNA binding of ecdysone receptor and ultraspiracle. Arch Insect Biochem Physiol 65:125–133PubMedGoogle Scholar
  24. 24.
    Nieva C, Spindler-Barth M, Spindler K-D (2007) Influence of hormone on intracellular localization of the Drosophila melanogaster ecdysteroid receptor (EcR). Cell Signal 19:2582–2587PubMedGoogle Scholar
  25. 25.
    Beatty J, Fauth T, Callender JL, Spindler-Barth M, Henrich VC (2006) Analysis of transcriptional activity mediated by the Drosophila melanogaster ecdysone receptor isoforms in a heterologous cell culture system. Insect Mol Biol 15:785–795PubMedGoogle Scholar
  26. 26.
    Ruff H, Tremmel Ch, Spindler-Barth M (2009) Transcriptional activity of ecdysone receptor isoforms is regulated by modulation of receptor stability and interaction with AB- and C-domains of the heterodimerization partner Ultraspiracle. Arch Insect Biochem Physiol (in press)Google Scholar
  27. 27.
    Nieva C, Spindler-Barth M, Spindler KD (2008) Impact of heterodimerization on intracellular localization of the ecdysteroid receptor (EcR). Arch Insect Biochem Physiol 68:40–48PubMedGoogle Scholar
  28. 28.
    Costantino BF, Bricker DK, Alexandre K, Shen K, Merriam JR, Antoniewski C, Callender JL, Henrich VC, Presente A, Andres AJ (2008) A novel ecdysone receptor mediates steroid-regulated developmental events during the mid-third instar of Drosophila. PLoS 4:e1000102Google Scholar
  29. 29.
    Grebe M, Rauch P, Spindler-Barth M (2000) Characterization of subclones of the epithelial cell line from Chironomus tentans resistant to the insecticide RH 5992, a non-steroidal moulting hormone agonist. Insect Biochem Mol Biol 30:591–600PubMedGoogle Scholar
  30. 30.
    Ogura T, Minakuchi C, Nakagawa Y, Smagghe G, Miyagawa H (2005) Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata. FEBS J 272:4114–4128PubMedGoogle Scholar
  31. 31.
    Nakagawa Y, Sakai A, Magata F, Ogura T, Miyashita M, Miyagawa H (2007) Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae. FEBS J 274:6191–6203PubMedGoogle Scholar
  32. 32.
    Nowickyj SM, Chithalen JV, Cameron D, Tyshenko MG, Petkovich M, Wyatt GR, Jone G, Walker VK (2008) Locust retinoid X receptors: 9-cis-retinoic acid in embryos from a primitive insect. Proc Natl Acad Sci USA 105:9540–9545PubMedGoogle Scholar
  33. 33.
    Laudet V, Bonneton F (2005) Evolution of nuclear hormone receptors in insects. In: Iatrou K, Gill SS (eds) Comprehensive insect physiology, biochemistry, and molecular biology series, vol 3. Elsevier, Oxford, pp 287–318Google Scholar
  34. 34.
    Billas I, Browning C, Lawrence MC, Graham LD, Moras D, Hill RJ (2009) The structure and function of ecdysone receptors. In: Smagghe G (ed) Ecdysteroids, structures and functions. Springer, Berlin, pp 335–375Google Scholar
  35. 35.
    Lafont R, Koolman J (2009) Diversity of ecdysteroids in animal species. In: Smagghe G (ed) Ecdysteroids, structures and functions. Springer, Berlin, pp 47–71Google Scholar
  36. 36.
    Azoitei A, Spindler-Barth M (2009) DNA affects ligand binding of the ecdysone receptor of Drosophila melanogaster. Mol Cell Endocrinol 303:91–99PubMedGoogle Scholar
  37. 37.
    Elke C, Rauch P, Spindler-Barth M, And Spindler K-D (1999) DNA-binding properties of the ecdysteroid receptor-complex (EcR/USP) of the epithelial cell line from Chironomus tentans. Arch Insect Biochem Physiol 41:124–133PubMedGoogle Scholar
  38. 38.
    Chandra V, Huang P, Hamuro Y, Raghuram S, Wang Y, Burris TP, Rastinejad F (2008) Structure of the intact PPAR–gamma-RXR-nuclear receptor complex on DNA. Nature 456:350–356PubMedGoogle Scholar
  39. 39.
    Jones G, Jones D, Teal P, Sapa A, Wozniak M (2006) The retinoid-X receptor ortholog, ultraspiracle, binds with nanomolar affinity to an endogenous morphogenetic ligand. FEBS J 273:4983–4996PubMedGoogle Scholar
  40. 40.
    Iwema T, Billas IM, Beck Y, Bonneton F, Nierengarten H, Chaumot A, Richards G, Laudet V, Moras D (2007) Structural and functional characterization of a novel type of ligand-independent RXR–USP receptor. EMBO J 26:3770–3782PubMedGoogle Scholar
  41. 41.
    Clayton GM, Peak-Chew SY, Evans RM, Schwabe JWR (2001) The structure of the ultraspiracle ligand-binding domain reveals a nuclear receptor locked in an inactive conformation. Proc Natl Acad Sci USA 98:1549–1554PubMedGoogle Scholar
  42. 42.
    Billas IML, Moulinier L, Rochel N, Moras D (2001) Crystal structure of the ligand binding domain of the ultraspiracle protein USP, the ortholog of RXRs in insects. J Biol Chem 276:7465–7474PubMedGoogle Scholar
  43. 43.
    Henrich VC, Beatty JM, Ruff H, Callender J, Grebe M, Spindler-Barth M (2009) The multidimensional partnership of EcR and USP. In: Smagghe G (ed) Ecdysones, structures and functions. Springer, BerlinGoogle Scholar
  44. 44.
    Varghese J, Cohen SM (2007) MicroRNA miR-14 acts to modulate a positive autoregulatory loop controlling steroid hormone signaling in Drosophila. Genes Dev 21:2277–2282PubMedGoogle Scholar
  45. 45.
    Shirai H, Kamimura M, Fujiwara H (2007) Characterization of core promoter elements for ecdysone receptor isoforms of the silkworm, Bombyx mori. Insect Mol Biol 16:253–264PubMedGoogle Scholar
  46. 46.
    Azoitei A, Ruff H, Tremmel Ch, Braun S, Spindler-Barth M (2009) Functional analysis of ecdysteroid receptor from Drosophila melangoaster “In Vitro”. In: Smagghe G (ed) Ecdysones, structures and functions. Springer, Berlin, pp 377–388Google Scholar
  47. 47.
    Cherbas L, Hu X, Zhimulyaeva J, Cherbas P (2003) EcR isoforms in Drosophila: testing tissue-specific requirements by targeted blockade and rescue. Development 130:271–284PubMedGoogle Scholar
  48. 48.
    Graham LD, Pilling PA, Eaton RE, Gorman JJ, Braybrook C, Hannan GN, Pawlak-Skrzecz A, Noyce L, Lovrecz GO, Lu L, Hill RJ (2007) Purification and characterization of recombinant ligand-binding domains from the ecdysone receptors of four pest insects. Protein Exp Purif 53:309–324Google Scholar
  49. 49.
    Graham LD, Johnson WM, Pawlak-Skrzeca A, Eaton RE, Bliese M, Howell L, Hannan GN, Hill RJ (2007) Ligand binding by recombinant domains from insect ecdysone receptors. Insect Biochem Mol Biol 37:611–626PubMedGoogle Scholar
  50. 50.
    Spindler K-D, Betanska K, Nieva C, Gwozdz T, Dutko-Gwozdz J, Ozyhar A, Spindler-Barth M (2009) Intracellular localization of the ecdysteroid receptor. In: Smagghe G (ed) Ecdysteroids, structures and functions. Springer, Berlin, pp 389–409Google Scholar
  51. 51.
    Dutko-Gwóźdź J, Gwóźdź T, Orłowski M, Greb-Markiewicz B, Duś D, Dobrucki J, Ozyhar A (2008) The variety of complexes formed by EcR and Usp nuclear receptors in the nuclei of living cells. Mol Cell Endocrinol 294:45–51PubMedGoogle Scholar
  52. 52.
    Betanska K, Nieva C, Spindler-Barth M, Spindler K-D (2007) Nucleocytoplasmic shuttling of the ecdysteroid receptor (EcR) and of ultraspiracle (Usp) from Drosophila melanogaster in mammalian cells: energy requirement and interaction with exportin. Arch Insect Biochem Physiol 65:134–142PubMedGoogle Scholar
  53. 53.
    Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F (2003) Structure of the heterodimeric ecdysone receptor DNA-binding complex. EMBO J 22:5827–5840PubMedGoogle Scholar
  54. 54.
    Krusinski T, Laskowska A, Ozyhar A, Dobryszycki P (2008) The application of an immobilized molecular beacon for the analysis of the DNA binding domains from the ecdysteroid receptor proteins Usp and EcR’s interaction with the hsp27 response element. J Biomol Screen 13:899–905PubMedGoogle Scholar
  55. 55.
    Krusiński T, Wietrzych M, Grad I, Ozyhar A, Dobryszycki P (2008) Equilibrium analysis of the DNA binding domain of the ultraspiracle protein interaction with the response element from the hsp27 gene promoter—the application of molecular beacon technology. J Fluoresc 18:1–10PubMedGoogle Scholar
  56. 56.
    Jakób M, Kołodziejczyk R, Orłowski M, Krzywda S, Kowalska A, Dutko-Gwóźdź J, Gwóźdź T, Kochman M, Jaskólski M, Ozyhar A (2007) Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain. Nucleic Acids Res 35:2705–2718PubMedGoogle Scholar
  57. 57.
    Orlowski M, Szyszka M, Kowalska A, Grad I, Zoglowek A, Rymarczyk G, Dobryszycki P, Krowarsch D, Rastinejad F, Kochman M, Ozyhar A (2004) Plasticity of the ecdysone receptor DNA binding domain. Mol Endocrinol 18:2166–2184PubMedGoogle Scholar
  58. 58.
    Niedziela-Majka A, Kochman M, Ozyhar A (2000) Polarity of the ecdysone receptor complex interaction with the palindromic response element from the hsp27 gene promoter. Eur J Biochem 267:507–519PubMedGoogle Scholar
  59. 59.
    Dobryszycki P, Grad I, Krusiński T, Michaluk P, Sawicka D, Kowalska A, Orłowski M, Jakób M, Rymarczyk G, Kochman M, Ozyhar A (2006) The DNA-binding domain of the ultraspiracle drives deformation of the response element whereas the DNA-binding domain of the ecdysone receptor is responsible for a slight additional change of the preformed structure. Biochemistry 45:668–675PubMedGoogle Scholar
  60. 60.
    Schubiger M, Carré C, Antoniewski C, Truman JW (2005) Ligand-dependent de-repression via EcR/USP acts as a gate to coordinate the differentiation of sensory neurons in the Drosophila wing. Development 132:5239–5248PubMedGoogle Scholar
  61. 61.
    Kumar R, Thompson EB (2003) Transactivation of the N-terminal domains of nuclear receptors: protein folding and coactivator interactions. Mol Endocrinol 17:1–10PubMedGoogle Scholar
  62. 62.
    Wurtz J-M, Bourguet W, Renaud J-P, Vivat V, Cambon P, Moras D, Gronemeyer H (1995) A canonical structure for the ligand-binding domain of nuclear receptors. Nat Struct Biol 3:87–94Google Scholar
  63. 63.
    Wurtz J-M, Bourguet W, Renaud J-P, Vivat V, Cambon P, Moras D, Gronemeyer H (1995) Erratum. Nat Struct Biol 3:206Google Scholar
  64. 64.
    Dressel U, Thormeyer D, Altincicek B, Paululat A, Eggert M, Schneider S, Tenbaum SP, Renkawitz R, Baniahmad A (1999) Alien, a highly conserved protein with characteristics of a corepressor for members of the nuclear hormone receptor superfamily. Mol Cell Biol 19:3383–3394PubMedGoogle Scholar
  65. 65.
    Ivaldi MS, Karam CS, Corce VG (2007) Phosphorylation of histone H3 at Ser10 facilitates RNA polymerase II release from promoter-proximal pausing in Drosophila. Genes Dev 21:2818–2831PubMedGoogle Scholar
  66. 66.
    Kimura S, Sawatsubashi S, Ito S, Kouzmenko A, Suzuki E, Zhao Y, Yamagata K, Tanabe M, Ueda T, Fujiyama S, Murata T, Matsukawa H, Takeyama K, Yaegashi N, Kato S (2008) Drosophila arginine methyltransferase 1 (DART1) is an ecdysone receptor co-repressor. Biochem Biophys Res Commun 371:889–893PubMedGoogle Scholar
  67. 67.
    Agawa Y, Sarhan M, Kageyama Y, Akagi K, Takai M, Hashiyama K, Wada T, Handa H, Iwamatsu A, Hirose S, Ueda H (2007) Drosophila Blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway. Mol Cell Biol 27:8739–8747PubMedGoogle Scholar
  68. 68.
    Song LN, Herrell R, Byers S, Shah S, Wilson EM, Gelmann EP (2003) Beta-catenin binds to the activation function 2 region of the androgen receptor and modulates the effects of the N-terminal domain and TIF2 on ligand-dependent transcription. Mol Cell Biol 23:1674–1687PubMedGoogle Scholar
  69. 69.
    Sedkov Y, Cho E, Petruk S, Cherbas L, Smith ST, Jones RS, Cherbas P, Canaani E, Jaynes JB, Mazo A (2003) Methylation at lysine 4 of histone H3 in ecdysone-dependent development of Drosophila. Nature 426:78–83PubMedGoogle Scholar
  70. 70.
    Zhu J, Chen L, Sun G, Raikhel AS (2006) The competence factor beta Ftz-F1 potentiates ecdysone receptor activity via recruiting a p160/SRC coactivator. Mol Cell Biol 26:9402–9412PubMedGoogle Scholar
  71. 71.
    Stabell M, Eskeland R, Bjørkmo M, Larsson J, Aalen RB, Imhof A, Lambertsson A (2006) The Drosophila G9a gene encodes a multi-catalytic histone methyltransferase required for normal development. Nucleic Acids Res 200634:4609–4621Google Scholar
  72. 72.
    Badenhorst P, Xiao H, Cherbas L, Kwon SY, Voas M, Rebay I, Cherbas P, Wu C (2005) The Drosophila nucleosome remodeling factor NURF is required for ecdysteroid signaling and metamorphosis. Genes Dev 19:2540–2545PubMedGoogle Scholar
  73. 73.
    Chen J, Wang H, Wang YF (2008) Overexpression of HmgD causes the failure of pupariation in Drosophila by affecting ecdysone receptor pathway. Arch Insect Biochem Physiol 68:123–133PubMedGoogle Scholar
  74. 74.
    Koolman J, Spindler K-D (1983) Mechanism of action of ecdysteroids. In: Downer RGH, Laufer H (eds) Invertebrate endocrinology—endocrinology of insects. Alan Liss, New York, pp 179–201Google Scholar
  75. 75.
    Tomaschko K-H (1999) Nongenomic effects of ecdysteroids. Arch Insect Biochem Physiol 41:89–98Google Scholar
  76. 76.
    Schlattner U, Vafopoulo X, Steel CGH, Hormann RE, Lezzi M (2006) Non-genomic ecdysone effects and the invertebrate nuclear steroid hormone receptorEcR–new role for an “old” receptor? Mol Cell Endocrinol 247:64–72PubMedGoogle Scholar
  77. 77.
    Elmogy M, Iwami M, Sakurai S (2004) Presence of membrane ecdysone receptor in the anterior silk gland of the silkworm Bombyx mori. Eur J Biochem 271:3171–3179PubMedGoogle Scholar
  78. 78.
    Elmogy M, Terashima J, Iga M, Iwami M, Sakurai S (2006) A rapid increase in cAMP in response to 20-hydroxyecdysone in the anterior silk glands of the silkworm, Bombyx mori. Zool Sci 23:715–719PubMedGoogle Scholar
  79. 79.
    Evans PD, Srivastava DP, Reale V (2009) Rapid, nongenomic tresponses to ecdysteroids and catecholamines mediated by a novel Drosophila G-protein-coupled receptor. In: Smagghe G (ed) Ecdysteroids, structures and functions. Springer, Berlin, pp 425–443Google Scholar
  80. 80.
    Flatt T, Heyland A, Rus F, Porpiglia E, Sherlock C, Yamamoto R, Garbuzov A, Palli SR, Tatar M, Silverman N (2008) Hormonal regulation of the humoral innate immune response in Drosophila melanogaster. J Exp Biol 211:2712–2724PubMedGoogle Scholar
  81. 81.
    Steward R (1998) Relocalization of the dorsal protein from the cytoplasm to the nucleus correlates with its function. Cell 59:1179–1188Google Scholar
  82. 82.
    Mirth CK, Riddiford LM (2007) Size assessment and growth control: how adult size is determined in insect. Bioessays 29:344–355PubMedGoogle Scholar
  83. 83.
    Orme MH, Leevers SJ (2005) Flies on steroids: The interplay between ecdysone and insulin signalling. Cell Metab 277–78. doi:10.11016/j.cmet.2005.10.005
  84. 84.
    Mirth CK, Truman JW, Riddiford LM (2005) The role of the prothoracic gland in determining critical weight for metamorphosis in Drosophila melanogaster. Curr Biol 15:1796–1807PubMedGoogle Scholar
  85. 85.
    McBrayer Z, Ono H, Shimell M-J, Parvy JP, Beckstead RB, Warren JT, Thummel CS, Dauphin-Villemant C, Gilbert LI, O`Connor MB (2007) Prothoracicotropic hormone regulates developmental timing and body size in Drosophila. Dev Cell 13:857–871PubMedGoogle Scholar
  86. 86.
    Truman JW, Hiruma K, Allee JP, MacWhinnie SBG, Champlin D, Riddiford L (2006) Juvenile hormone is required to couple imaginal disc formation with nutrition in insects. Science 310:667–670Google Scholar
  87. 87.
    Kaplan DD, Zimmermann G, Suyama K, Meyer T, Scott MP (2008) A nucleostemin family GTPase, NS3, acts in serotinergic neurons to regulate insulin signalling and con troll body size. Genes Dev. doi: 10.1101/gad.1670508
  88. 88.
    Hatt PJ, Liebon C, Morinière M, Porcheron P (1994) Roles for insulin and ecdysteroids in differentiation on an insect cell line of epidermal origin. In Vitro Cell Dev Biol Anim 30A:717–720PubMedGoogle Scholar
  89. 89.
    Vander Griend DJ, Litvinov IV, Isaacs JT (2007) Stabilizing androgen receptor in mitosis inhibits prostate cancer proliferation. Cell Cycle 15:647–651Google Scholar
  90. 90.
    Maruvada P, Baumann CT, Hager GL, Yen PM (2003) Cell cycle-dependent expression of thyroid hormone receptor-beta is a mechanism for variable hormone sensitivity. Mol Biol Cell 15:1895–1903Google Scholar
  91. 91.
    Narayanan R, Edwards DP, Weigel NL (2005) Human progesterone receptor displays cell cycle-dependent changes in transcriptional activity. Mol Cell Biol 25:2885–2898PubMedGoogle Scholar
  92. 92.
    Zwijsen RM, Buckle RS, Hijmans EM, Loomans CJ, Bernards R (1998) Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev 12:3488–3498PubMedGoogle Scholar
  93. 93.
    Petre-Draviam CE, Cook SL, Burd CJ, Marshall TW, Wetherill YB, Knudsen KE (2003) Specificity of cyclin D1 for androgen receptor regulation. Cancer Res 63:4903–4913PubMedGoogle Scholar
  94. 94.
    Parthasarathy R, Palli SR (2008) Proliferation and differentiation of intestinal stem cells during metamorphosis of the red flour beetle, Tribolium castaneum. Dev Dyn 237:893–908PubMedGoogle Scholar
  95. 95.
    Champlin DT, Truman JW (1998) Ecdysteroid control of cell proliferation during optic lobe neurogenesis in the moth Manduca sexta. Development 125:269–277PubMedGoogle Scholar
  96. 96.
    Spindler-Barth M, Junger E, Spindler K-D (1992) Vesicle formation and ecdysteroid induced cellular differentiation in the epithelial cell line from Chironomus tentans. Tissue Cell 24:919–934Google Scholar
  97. 97.
    Swiderski RE, O’Connor JD (1986) Modulation of novel-length DOPA decarboxylase transcripts by 20-OH-ecdysone in a Drosophila melanogaster Kc cell subline. Mol Cell Biol 6:4433–4439PubMedGoogle Scholar
  98. 98.
    Mottier V, Siaussat D, Bozzolan F, Auzoux-Bordenave S, Porcheron P, Debernard S (2004) The 20-hydroxyecdysone-induced cellular arrest in G2 phase is preceded by an inhibition of cyclin expression. Insect Biochem Mol Biol 34:51–60PubMedGoogle Scholar
  99. 99.
    Gerenday A, Fallon AM (2004) Ecdysone-induced accumulation of mosquito cells in the G1 phase of the cell cycle. J Insect Physiol 50:831–838PubMedGoogle Scholar
  100. 100.
    Siaussat D, Bozzolan F, Queguiner I, Porcheron P, Debernard S (2005) Cell cycle profiles of EcR, USP, HR3 and B cyclin mRNAs associated to 20E-induced G2 arrest of Plodia interpunctella imaginal wing cells. Insect Mol Biol 14:151–161PubMedGoogle Scholar
  101. 101.
    Siaussat D, Bozzolan F, Porcheron P, Debernard S (2008) The 20-hydroxyecdysone-induced signalling pathway in G2/M arrest of Plodia interpunctella imaginal wing cells. Insect Biochem Mol Biol 38:529–539PubMedGoogle Scholar
  102. 102.
    Betanska K, Czogalla S, Spindler-Barth M, Spindler KD (2009) Cell cycle-dependent ecdysteroid localization and concentration in chinese hamster ovary cells. Arch Insect Biochem Physiol (in press)Google Scholar
  103. 103.
    Siaussat D, Porcheron P, Debernard S (2009) In: Smagghe G (ed) Ecdysteroids, structures and functions. Springer, Berlin, pp 185–204Google Scholar
  104. 104.
    Parthasarathy R, Palli SR (2007) Stage- and cell-specific expression of ecdysone receptors and ecdysone-induced transcription factors during midgut remodeling in the yellow fever mosquito, Aedes aegypti. J Insect Physiol 53:216–229PubMedGoogle Scholar
  105. 105.
    Parthasarathy R, Palli SR (2007) Developmental and hormonal regulation of midgut remodeling in a lepidopteran insect, Heliothis virescens. Mech Dev 124:23–34PubMedGoogle Scholar
  106. 106.
    Wu Y, Parthasarathy R, Bai H, Palli SR (2006) Mechanisms of midgut remodeling: juvenile hormone analog methoprene blocks midgut metamorphosis by modulating ecdysone action. Mech Dev 123:530–547PubMedGoogle Scholar
  107. 107.
    Parthasarathy R, Tan A, Bai H, Palli SR (2008) Transcription factor broad suppresses precocious development of adult structures during larval-pupal metamorphosis in the red flour beetle, Tribolium castaneum. Mech Dev 125:299–313PubMedGoogle Scholar
  108. 108.
    Parthasarathy R, Tan A, Palli SR (2008) bHLH-PAS family transcription factor methoprene-tolerant plays a key role in JH action in preventing the premature development of adult structures during larval–pupal metamorphosis. Mech Dev 125:601–616PubMedGoogle Scholar
  109. 109.
    Berger EM, Dubrovsky EB (2005) Juvenile hormone molecular actions and interactions during development of Drosophila melanogaster. Vitam Horm 73:175–215PubMedGoogle Scholar
  110. 110.
    Barchuk AR, Figueiredo VL, Simões ZL (2008) Downregulation of ultraspiracle gene expression delays pupal development in honeybees. J Insect Physiol 54:1035–1040PubMedGoogle Scholar
  111. 111.
    Konopova B, Jindra M (2007) Juvenile hormone resistance gene Methoprene-tolerant controls entry into metamorphosis in the beetle Tribolium castaneum. Proc Natl Acad Sci USA 104:10488–10493PubMedGoogle Scholar
  112. 112.
    Konopova B, Jindra M (2008) Broad-complex acts downstream of Met in juvenile hormone signaling to coordinate primitive holometabolan metamorphosis. Development 135:559–568PubMedGoogle Scholar
  113. 113.
    Li Y, Zhang Z, Robinson GE, Palli SR (2007) Identification and characterization of a juvenile hormone response element and its binding proteins. J Biol Chem 282:37605–37617PubMedGoogle Scholar
  114. 114.
    Maki A, Sawatsubashi S, Ito S, Shirode Y, Suzuki E, Zhao Y, Yamagata K, Kouzmenko A, Takeyama K, Kato S (2004) Juvenile hormones antagonize ecdysone actions through co-repressor recruitment to EcR/USP heterodimers. Biochem Biophys Res Commun 320:262–267PubMedGoogle Scholar
  115. 115.
    Henrich VC, Burns E, Yelverton DP, Christensen E, Weinberger C (2003) Juvenile hormone potentiates ecdysone receptor-dependent transcription in a mammalian cell culture system. Insect Biochem Mol Biol 33:1239–1247PubMedGoogle Scholar
  116. 116.
    Planelló R, Martínez-Guitarte JL, Morcillo G (2008) The endocrine disruptor bisphenol A increases the expression of HSP70 and ecdysone receptor genes in the aquatic larvae of Chironomus riparius. Chemosphere 71:1870–1876PubMedGoogle Scholar
  117. 117.
    Lye CM, Bentley MG, Galloway T (2008) Effects of 4-nonylphenol on the endocrine system of the shore crab, Carcinus maenas. Environ Toxicol 23:309–318PubMedGoogle Scholar
  118. 118.
    Le Goff G, Hilliou F, Siegfried BD, Boundy S, Wajnberg E, Sofer L, Audant P, ffrench-Constant RH, Feyereisen R (2006) Xenobiotic response in Drosophila melanogaster: sex dependence of P450 and GST gene induction. Insect Biochem Mol Biol 36:674–682PubMedGoogle Scholar
  119. 119.
    King-Jones K, Horner MA, Lam G, Thummel CS (2006) The DHR96 nuclear receptor regulates xenobiotic responses in Drosophila. Cell Metab 4:37–48PubMedGoogle Scholar
  120. 120.
    Bathori M, Toth N, Hunyadi A, Marki A, Zador E (2008) Phytoecdysteroids and anabolic-androgenic steroids–structure and effects in humans. Curr Med Chem 15:75–91PubMedGoogle Scholar
  121. 121.
    Lafont R, Dinan L (2009) Innovative and future applications for ecdysteroids. In: Smagghe G (ed) Ecdysteroids, structures and functions. Springer, Berlin, pp 551–578Google Scholar
  122. 122.
    Dhadialla TS, Carlson GR, Lee DP (1998) New insecticides with ecdysteroidal and juvenile hormone activity. Annu Rev Entomol 43:545–569PubMedGoogle Scholar
  123. 123.
    Mommaerts V, Sterk G, Smagghe G (2006) Bumblebees can be used in combination with juvenile hormone analogues and ecdysone agonists. Ecotoxicology 15:513–521PubMedGoogle Scholar
  124. 124.
    Harmatha J, Dinan L (1997) Biological activity of natural and synthetic ecdysteroids in the BII bioassay. Arch Insect Biochem Physiol 35:219–225PubMedGoogle Scholar
  125. 125.
    Li J, Qi H, Qi LW, Yi L, Li P (2007) Simultaneous determination of main phytoecdysones and triterpenoids in radix achyranthis bidentatae by high-performance liquid chromatography with diode array-evaporative light scattering detectors and mass spectrometry. Anal Chim Acta 596:264–272PubMedGoogle Scholar
  126. 126.
    Carmichael JA, Lawrence MC, Graham LD, Pilling PA, Epa VC, Noyce L, Lovrecz G, Windler DA, Pawlak-Skrezecz A, Eaton RE, Hannan GN, Hill RJ (2005) The X-ray structure of a hemipteran ecdysone receptor ligand-binding domain: comparison with a lepidopteran ecdysone receptor ligand-binding domain and implications for insecticide design. J Biol Chem 280:22258–22269PubMedGoogle Scholar
  127. 127.
    Lapenna S, Friz J, Barlow A, Palli SR, Dinan L, Hormann RE (2008) Ecdysteroid ligand–receptor selectivity—exploring trends to design orthogonal gene switches. FEBS J. 275:5785–5809PubMedGoogle Scholar
  128. 128.
    Fujita T, Nakagawa Y (2007) QSAR and mode of action studies of insecticidal ecdysone agonists. SAR QSAR Environ Res 18:77–88PubMedGoogle Scholar
  129. 129.
    Bordas B, Belai I, Lopata A, Szanto Z (2007) Interpretation of scoring functions using 3D molecular fields. Mapping the diacyl-hydrazine-binding pocket of an insect ecdysone receptor. J Chem Inf Model 47:176–185PubMedGoogle Scholar
  130. 130.
    Holmwood G, Schindler M (2009) Protein structure based rational design of ecdysone agonists. Bioorg Med Chem 17:4046–4070Google Scholar
  131. 131.
    Dhadialla TS, Le D, Palli SR, Raikhel A, Carlson GR (2007) A photoaffinity, non-steroidal, ecdysone agonist, RH-131039: characterization of binding and functional activity. Insect Biochem Mol Biol 37:865–875PubMedGoogle Scholar
  132. 132.
    Gu JB, Sun YT, Peng HJ (2007) Construction of a yeast model for screening Aedes albopictus ecdysone agonist pesticides. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 25:376–380PubMedGoogle Scholar
  133. 133.
    Beatty J, Smagghe G, Ogura T, Nakagawa Y, Spindler-Barth M, Henrich VC (2009) Ecdysteroid receptor properties of two species from different insect orders compared in a heterologous cell culture system. FEBS J 276:3087–3098PubMedGoogle Scholar
  134. 134.
    Spindler-Barth M, Spindler K-D (1998) Ecdysteroid resistant subclones of the epithelial cell line from Chironomus tentans (Insecta, Diptera). I. Selection and characterization of resistant clones. In Vitro Cell Dev Biol Anim 34:116–122PubMedGoogle Scholar
  135. 135.
    Kayser H, Winkler T, Spindler-Barth M (1997) 26-hydroxylation of ecdysteroids is catalyzed by a typical cytochrome P-450-dependent oxidase and related to ecdysteroid resistance in an insect cell line. Eur J Biochem 248:707–716PubMedGoogle Scholar
  136. 136.
    Swevers L, Soin T, Mosallanejad H, Iatrou K, Smagghe G (2008) Ecdysteroid signaling in ecdysteroid-resistant cell lines from the polyphagous noctuid pest Spodoptera exigua. Insect Biochem Mol Biol 38:825–833PubMedGoogle Scholar
  137. 137.
    Retnakaran A, Gelbic I, Sundaram M, Tomkins W, Ladd T, Primavera M, Feng Q, Arif B, Palli R, Krell P (2001) Mode of action of the ecdysone agonist tebufenozide (RH-5992), and an exclusion mechanism to explain resistance to it. Pest Manag Sci 57:951–957PubMedGoogle Scholar
  138. 138.
    Retnakaran A, Krell P, Feng Q, Arif B (2003) Ecdysone agonists: mechanism and importance in controlling insect pests of agriculture and forestry. Arch Insect Biochem Physiol 54:187–199PubMedGoogle Scholar
  139. 139.
    Xie J, Nair A, Hermiston TW (2008) A comparative study examining the cytotoxicity of inducible gene expression system ligands in different cell types. Toxicol In Vitro 22:261–266PubMedGoogle Scholar
  140. 140.
    Panguluri SK, Kumar P, Palli SR (2006) Functional characterization of ecdysone receptor gene switches in mammalian cells. FEBS J 273:5550–5563PubMedGoogle Scholar
  141. 141.
    Magnenat L, Schwimmer LJ, Barbas CF III (2008). Drug-inducible and simultaneous regulation of endogenous genes by single-chain nuclear receptor-based zinc-finger transcription factor gene switches. Gene Ther 15:1223–1232 [erratum in: Gene Ther (2008) 15:1246]Google Scholar
  142. 142.
    Lessard J, Aicha SB, Fournier A, Calvo E, Lavergne E, Pelletier M, Labrie C (2007) Characterization of the RSL1-dependent conditional expression system in LNCap prostate cancer cells and development of a single vector format. Prostate 67:808–819PubMedGoogle Scholar
  143. 143.
    Tavva VS, Dinkins RD, Palli SR, Collins GB (2006) Development of a methoxyfenozide-responsive gene switch for applications in plants. Plant J 45:457–469PubMedGoogle Scholar
  144. 144.
    Tavva VS, Dinkins RD, Palli SR, Collins GB (2007) Development of a tightly regulated and highly inducible ecdysone receptor gene switch for plants through the use of retinoid X receptor chimeras. Transgenic Res 16:599–612PubMedGoogle Scholar
  145. 145.
    Tavva VS, Palli SR, Dinkins RD, Collins GB (2008) Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants. FEBS J 275:2161–2176PubMedGoogle Scholar
  146. 146.
    Kimbrell DA, Beutler B (2001) The evolution and genetics of innate immunity. Nat Rev Genet 2:256–267PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2009

Authors and Affiliations

  • Klaus-Dieter Spindler
    • 1
  • C. Hönl
    • 1
  • Ch. Tremmel
    • 1
  • S. Braun
    • 1
  • H. Ruff
    • 1
  • M. Spindler-Barth
    • 1
  1. 1.Institute of General Zoology and EndocrinologyUniversity of UlmUlmGermany

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