Cellular and Molecular Life Sciences

, Volume 65, Issue 19, pp 3081–3092 | Cite as

Recombinant scorpine: a multifunctional antimicrobial peptide with activity against different pathogens

  • R. Carballar-Lejarazú
  • M. H. Rodríguez
  • F. de la Cruz Hernández-Hernández
  • J. Ramos-Castañeda
  • L. D. Possani
  • M. Zurita-Ortega
  • E. Reynaud-Garza
  • R. Hernández-Rivas
  • T. Loukeris
  • G. Lycett
  • H. Lanz-Mendoza
Research Article

Abstract.

Scorpine is an antimicrobial peptide whose structure resembles a hybrid between a defensin and a cecropin. It exhibits antibacterial activity and inhibits the sporogonic development of parasites responsible for murine malaria. In this communication we report the production of scorpine in a heterelogous system, using a specific vector containing its cloned gene. The recombinantly expressed scorpine (RScp) in Anopheles gambie cells showed antibacterial activity against Bacillus subtilis and Klebsiella pneumoniae, at 5 and 10 μM, respectively. It also produced 98% mortality in sexual stages of Plasmodium berghei at 15 μM and 100% reduction in Plasmodium falciparum parasitemia at 5 μM. RScp also inhibited virus dengue-2 replication in C6/36 mosquito cells. In addition, we generated viable and fertile transgenic Drosophila that overexpresses and correctly secretes RScp into the insect hemolymph, suggesting that the generation of transgenic mosquitoes resistant to different pathogens may be viable.

Keywords.

Antimicrobial peptide dengue malaria Plasmodium recombinant scorpine virus 

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Copyright information

© Birkhaueser 2008

Authors and Affiliations

  • R. Carballar-Lejarazú
    • 1
  • M. H. Rodríguez
    • 1
  • F. de la Cruz Hernández-Hernández
    • 2
  • J. Ramos-Castañeda
    • 1
  • L. D. Possani
    • 3
  • M. Zurita-Ortega
    • 3
  • E. Reynaud-Garza
    • 3
  • R. Hernández-Rivas
    • 4
  • T. Loukeris
    • 5
  • G. Lycett
    • 5
  • H. Lanz-Mendoza
    • 1
  1. 1.Centro de Investigaciones sobre Enfermedades InfecciosasInstituto Nacional de Salud PúblicaCuernavacaMéxico
  2. 2.Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalDepartamento de Patología ExperimentalMéxicoMéxico
  3. 3.Instituto de BiotecnologíaUniversidad Nacional Autónoma de MéxicoSan AntonioMéxico
  4. 4.Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalDepartamento de Biomedicina MolecularMéxicoMéxico
  5. 5.Institute of Molecular Biology and BiotechnologyFoundation for Research and TechnologyHeraklion CreteGreece

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