Cellular and Molecular Life Sciences

, Volume 65, Issue 7–8, pp 1099–1122

Protein factors in pre-mRNA 3′-end processing


DOI: 10.1007/s00018-007-7474-3

Cite this article as:
Mandel, C.R., Bai, Y. & Tong, L. Cell. Mol. Life Sci. (2008) 65: 1099. doi:10.1007/s00018-007-7474-3


Most eukaryotic mRNA precursors (premRNAs) must undergo extensive processing, including cleavage and polyadenylation at the 3′-end. Processing at the 3′-end is controlled by sequence elements in the pre-mRNA (cis elements) as well as protein factors. Despite the seeming biochemical simplicity of the processing reactions, more than 14 proteins have been identified for the mammalian complex, and more than 20 proteins have been identified for the yeast complex. The 3′-end processing machinery also has important roles in transcription and splicing. The mammalian machinery contains several sub-complexes, including cleavage and polyadenylation specificity factor, cleavage stimulation factor, cleavage factor I, and cleavage factor II. Additional protein factors include poly(A) polymerase, poly(A)-binding protein, symplekin, and the C-terminal domain of RNA polymerase II largest subunit. The yeast machinery includes cleavage factor IA, cleavage factor IB, and cleavage and polyadenylation factor.


Protein complex cleavage and polyadenylation specificity factor cleavage stimulation factor poly(A) polymerase endoribonuclease RNA polymerase II 

Copyright information

© Birkhaueser 2007

Authors and Affiliations

  1. 1.Department of Biological SciencesColumbia UniversityNew YorkUSA

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