Cellular and Molecular Life Sciences

, Volume 64, Issue 24, pp 3282–3294 | Cite as

Role of transcriptional factors Sp1, c-Rel, and c-Jun in LPS-induced C/EBPδ gene expression of mouse macrophages

  • Y.-W. Liu
  • C.-C. Chen
  • J.-M. Wang
  • W.-C. Chang
  • Y.-C. Huang
  • S.-Y. Chung
  • B.-K. Chen
  • J.-J. Hung
Research Article

Abstract.

Transcription factor C/EBPs are involved in the regulation of various cellular responses. Here, it was suggested that C/EBPδ gene was activated by lipopolysaccharide (LPS) through transcription factors Sp1, c-Rel, and c-Jun. Assay of the luciferase reporter vectors containing a 5′-deletion of the C/EBPδ gene promoter indicated that a LPS-responsive element was positioned between –345 and –35 bp of mouse C/EBPδ gene promoter. Transcription factors Sp1, c-Rel, and c-Jun bound to this region were identified using both in vivo chromatin immunoprecipitation and in vitro DNA-protein binding assays. LPS enhanced the proteins and DNA binding capacities of c-Rel and c-Jun, and the downstream Sp1 site was essential for LPS-induced C/EBPδ gene. Treatment of cells with ERK/JNK/p38 inhibitors or NF-κB inhibitor inhibited the LPS-induced C/EBPδ gene expression by inhibiting c-Jun, c-Rel, and p300 binding to DNA. Our findings provide a better understanding of LPS-induced C/EBPδ gene expression.

Keywords.

Monocytes/macrophages transcription factors C/EBPδ Sp1 c-Rel c-Jun NF-κB MAPK LPS 

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Copyright information

© Birkhäuser Verlag, Basel 2007

Authors and Affiliations

  • Y.-W. Liu
    • 1
  • C.-C. Chen
    • 1
  • J.-M. Wang
    • 2
    • 3
  • W.-C. Chang
    • 3
    • 4
  • Y.-C. Huang
    • 1
  • S.-Y. Chung
    • 1
  • B.-K. Chen
    • 2
    • 3
  • J.-J. Hung
    • 2
    • 3
  1. 1.Graduate Institute of Biomedical and Biopharmaceutical Sciences, College of Life SciencesNational Chiayi UniversityChiayiTaiwan
  2. 2.Institute of Biosignal TransductionNational Cheng Kung UniversityTainan 701Taiwan
  3. 3.Center for Gene Regulation and Signal Transduction, College of Bioscience and BiotechnologyNational Cheng Kung UniversityTainan 701Taiwan
  4. 4.Department of Pharmacology, College of MedicineNational Cheng Kung UniversityTainan 701Taiwan

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